Functional analysis of the antioxidant activity of immune-challenged Bombyx mori hemolymph extracts in the human epithelial Caco-2 cell line
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Humans use insects as food and traditional medicine for many years. Hemolymph is the circulating fluid of insects and is a key component of their immune system. However, limited information is available regarding hemolymph identification, development, and differentiation, as well as the related cellular immune responses. In a previous study, hemolymph extracts prepared from Bombyx mori larvae were found to exert anti-inflammatory effects. In this study, we aimed to identify and compare the antioxidant activity of immune-challenged and unchallenged B. mori hemolymph extracts in vitro. For this purpose, human epithelial Caco-2 cells were first exposed to oxidative stress and then treated with various concentrations and incubation times of either immune-challenged or unchallenged B. mori hemolymph extracts. Next, we determined the effect of treatment on the relative expression of GPX-1, SOD-1, and SOD-2 antioxidant marker genes. We found that the expression rates of the three marker genes were markedly higher at a immune-challenged hemolymph extract concentration of 80 ppm compared to those at other concentrations, and the antioxidant effects were enhanced after treatment for 48 hr. Thus, B. mori hemolymph extracts showed antioxidant activity within the limited time and dose. Especially, the immune-challenged B. mori hemolymph extracts showed higher the antioxidant activities than unchallenged one. The activity of silkworm hemolymph extracts could facilitate the development of new types of functional foods, feed additives, and biomaterials with antioxidant properties.
Abstract
Humans use insects as food and traditional medicine for many years. Hemolymph is the circulating fluid of insects and is a key component of their immune system. However, limited information is available regarding hemolymph identification, development, and differentiation, as well as the related cellular immune responses. In a previous study, hemolymph extracts prepared from Bombyx mori larvae were found to exert anti-inflammatory effects. In this study, we aimed to identify and compare the antioxidant activity of immune-challenged and unchallenged B. mori hemolymph extracts in vitro. For this purpose, human epithelial Caco-2 cells were first exposed to oxidative stress and then treated with various concentrations and incubation times of either immune-challenged or unchallenged B. mori hemolymph extracts. Next, we determined the effect of treatment on the relative expression of GPX-1, SOD-1, and SOD-2 antioxidant marker genes. We found that the expression rates of the three marker genes were markedly higher at a immune-challenged hemolymph extract concentration of 80 ppm compared to those at other concentrations, and the antioxidant effects were enhanced after treatment for 48 hr. Thus, B. mori hemolymph extracts showed antioxidant activity within the limited time and dose. Especially, the immune-challenged B. mori hemolymph extracts showed higher the antioxidant activities than unchallenged one. The activity of silkworm hemolymph extracts could facilitate the development of new types of functional foods, feed additives, and biomaterials with antioxidant properties.
참고문헌 (32)
- Aono A, "Potent ectopic bone-inducing activity of bone morphogenetic protein-4", 7 heterodimer, 1995, pp.210, 670~677
- Balakrishnan D, "A review on antioxidant activity of marine organisms", Int J Chem Tech Res, 6(7), pp.3431~3436 (2014)
- Barbehenn RV, "Gut-based antioxidant enzymes in a polyphagous and a graminivorous grasshopper", J Chem Ecol, 28, pp.1329~1347 (2002)
- Baynes JW, "Role of oxidative stress in development of complications in diabetes", Diabetes, 40(4), pp.405~412 (1991)
- Chen Y, "A genetic screen for modifiers of Drosophila decapentaplegic signaling identifies mutations in punt, Mothers against dpp and the BMP-7 homologue, 60A", Development, 125, pp.1759~1768 (1998)
- Chew BP, "Carotenoid action on the immune response", J Nutr, 134(1), pp.257~ (2004)
- Dhinaut J, "A dietary carotenoid reduces immunopathology and enhances longevity through an immune depressive effect in an insect model", Sci Rep, 7, pp.12429~ (2017)
- Felton GW, "Antioxidant systems in insects", Arch Insect Biochem Physiol, 29(2), pp.187~197 (1995)
- Fridovich I, "The biology of oxygen radicals", Science, 201(4359), pp.875~880 (1978)
- 김성렬, "Antibacterial effects of two cecropin type peptides isolated from the silkworm against Salmonella species", International Journal of Industrial Entomology and Biomaterials, 37(2), pp.95~99 (2018), 한국잠사학회
- 김성률, "Antibacterial and anti-inflammatory activities of the immunechallenged silkworm (Bombyx mori) hemolymph with Lactobacillus cell wall extracts", Entomological Research, 49(8), pp.354~362 (2019), 한국곤충학회
- Kim YI, "Bombyx mori hemocyte extract has anti-inflammatory effects on human phorbol myristate acetate-differentiated THP-1 cells via TLR4-mediated suppression of the NF-κB signaling pathway", Mol Med Rep, 16, pp.4001~4007 (2017)
- Künnapuu J, Shimmi O, "Evolutional imprints on the sequences of BMP2", 4, Fly (Austin), pp.2010, 4[DOI]
- Landis GN, "Superoxide dismutase evolution and life span regulation", Mech Ageing Dev, 126(3), pp.365~379 (2005)
- Lettieri-Barbato D, "Effect of plant foods and beverages on plasma non-enzymatic antioxidant capacity in human subjects : a meta-analysis", Br J Nutr, 109, pp.1544~1556 (2013)
- Magrone T, "Functional foods and nutraceuticals as therapeutic tools for the treatment of diet-related diseases", Can J Physiol Pharmacol, 91, pp.387~396 (2013)
- Mason JB, "Fostering strategies to expand the consumption of edible insects : the value of a tripartite coalition between academia, industry, and government", Curr Dev Nutr, 2, pp.1~5 (2018)
- Mattia CD, "Antioxidant activities in vitro of water and liposoluble extracts obtained by different species of edible insects and invertebrates", Front Nutr, 6, pp.106~ (2019)
- Mittapalli O, "Antioxidant defense response in a galling insect", Proc Natl Acad Sci U S A, 104(6), pp.1889~1894 (2007)
- Mookherjee N, "Modulation of the TLR-mediated inflammatory response by the endogenous human host defense peptide LL-37", J Immunol, 176, pp.2455~2464 (2006)
- Morath S, "Structure-function relationship of cytokine induction by lipoteichoic acid from Staphylococcus aureus", J Exp Med, 193, pp.393~397 (2001)
- Munford RS, "Normal responses to injury prevent systemic inflammation and can be immunosuppressive", Am J Respir Criti Care Med, 163, pp.316~321 (2001)
- Oghenesuvwe EE, "Edible insects bio-actives as antioxidants : Current status and perspectives", J Complement Med Res, 10(2), pp.89~102 (2019)
- Pardini RS, "Toxicity of oxygen from naturally occurring redoxactive pro-oxidants", Arch Insect Biochem Physiol, 29(2), pp.101~118 (1995)
- Pimentel D, "Sustainability of meat-based and plantbased diets and the environment", Am J Clin Nutr, 78(Suppl) (2003)
- Serafini M, "Plasma antioxidants from chocolate", Nature, 424, pp.1013~ (2003)
- Serafini M, "Modulation of plasma non-enzimatic antioxidant capacity(NEAC)by plant foods : the role of polyphenols", Curr Top Med Chem, 11, pp.1821~1846 (2011)
- Sosa V, "Oxidative stress and cancer : an overview", Ageing Res Rev, 12(1), pp.376~390 (2013)
- Van Huis A, "Edible Insects", Future Prospects for Food and Feed Security, 171, pp.60~64 (2013), Food and Agriculture Organization of the United Nations
- Yoon KY, "Brazilin suppresses inflammation via the down-regulation of IRAK4 in LPSstimulated Raw264.7 macrophage", J Food Nutr Res, 3(9), pp.575~580 (2015)
- Zielińska E, "Antioxidant activity of predigested protein obtained from a range of farmed edible insects", Int J Food Sci Tech, 52(2), pp.306~312 (2017)
- Zhang Y, "Antioxidant enzyme activities and lipid oxidation in rape (Brassica campestris L.) bee pollen added to salami during processing", Molecules, 21(11) (2016)