본문 바로가기
KERD
논문KCI 등재

Immune-enhancing effects of Protaetia brevitarsis seulensis larvae extracts on RAW264.7 macrophages

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

초록

Protaetia brevitarsis seulensis larva is well-known as an edible insect. The present study aimed to explore the immune-enhancing effect of 30% ethanol extract of Protaetia brevitarsis seulensis larvae (PBE) in RAW264.7 macrophage cells. PBE were not cytotoxic to RAW264.7 cells and nitric oxide production increased on PBE treatment in a dose-dependent manner. Furthermore, PBE significantly promoted the expression of immune-related mediators (iNOS and COX-2) and cytokines (TNF-α, IL-6, and IL-1β) and the phosphorylation of MAPKs (ERK, p38, and JNK). Taken together, the immune-enhancing effects of 30% ethanol extract of PBE in vitro were identified. These findings can be used as data for the development of edible insect-based functional foods that improve immune function.

Abstract

Protaetia brevitarsis seulensis larva is well-known as an edible insect. The present study aimed to explore the immune-enhancing effect of 30% ethanol extract of Protaetia brevitarsis seulensis larvae (PBE) in RAW264.7 macrophage cells. PBE were not cytotoxic to RAW264.7 cells and nitric oxide production increased on PBE treatment in a dose-dependent manner. Furthermore, PBE significantly promoted the expression of immune-related mediators (iNOS and COX-2) and cytokines (TNF-α, IL-6, and IL-1β) and the phosphorylation of MAPKs (ERK, p38, and JNK). Taken together, the immune-enhancing effects of 30% ethanol extract of PBE in vitro were identified. These findings can be used as data for the development of edible insect-based functional foods that improve immune function.

참고문헌 (27)

  1. 안은미, "The ameliorative effect of Protaetia brevitarsis Larvae in HFD-induced obese mice", Food Science and Biotechnology, 28(4), pp.1177~1186 (2019), 한국식품과학회[DOI]
  2. Dong C, "MAP kinases in the immune response", Annu Rev Immunol, 20, pp.55~72 (2002)
  3. Chen J, "The application of fungal β-glucans for the treatment of colon cancer", Anti-Cancer Agents Med Chem, 13, pp.725~730 (2013)
  4. 추승빈, "Bacillus amyloliquefaciens (SRCM 100730)로 발효된 청국장 추출물의 RAW 264.7 대식세포 면역증강 활성", 한국식품영양과학회지, 46(11), pp.1300~1307 (2017), 한국식품영양과학회
  5. Mi Yeon Chung, "흰점박이꽃무지(Protaetia brevitarsis)의 일반성분 및 유해물질 분석", 생명과학회지, 23(5), pp.664~668 (2013), 한국생명과학회[DOI]
  6. Di Mattia C, "Antioxidant activities in vitro of water and loposoluble extracts obtained by different species of edible insects and invertebrates", Front Nutr, 6, pp.106~ (2019)
  7. Hong HD, "Immunoregulatory actions of polysaccahrides from natural plant resources", Bulletin of Food Technology, 24, pp.390~409 (2011)
  8. Huis AV, "Edible insects: future prospects for food and feed security" (2013), FAO
  9. 정근우, "흰점박이꽃무지 유충 추출물이 Co-60 감마선에 조사된 수컷 흰쥐의 혈구 및 전립선에 미치는 방사선 방호효과", 방사선기술과학, 44(2), pp.117~122 (2021), 대한방사선과학회
  10. Kim IS, "Essential Immunology" (2019), Daihaks
  11. Kim TK, "Development of new technology for functional materials for edible insects as alternative food", Livestock Food Science and Industry, 10, pp.1~13 (2021)
  12. 김혜수, "먹이원에 따른 흰점박이꽃무지(Protaetia brevitarsis) 유충의 항산화활성", 한국버섯학회지, 17(4), pp.261~267 (2019), 한국버섯학회[DOI]
  13. 김형숙, "Stimulatory Effect of β-glucans on Immune Cells", Immune Network, 11(4), pp.191~195 (2011), 대한면역학회
  14. 권은영, "흰점박이꽃무지(Protaetia brevitarsis)의 식품원료화를 위한 전처리 조건 확립", 한국식품영양과학회지, 42(3), pp.397~402 (2013), 한국식품영양과학회
  15. 이재훈, "In Vitro Immune-Enhancing Activity of Ovotransferrin from Egg White via MAPK Signaling Pathways in RAW 264.7 Macrophages", 한국축산식품학회지, 38(6), pp.1226~1236 (2018), 한국축산식품학회
  16. Lee JI, "Inhibition of platelet aggregation and thrombosis by indole alkaloids isolated from the edible insect Protaetia brevitarsis seulensis(Kolbe)", J Cell Med, 21, pp.1217~1227 (2017)
  17. Lee JK, "Acemannan purified from Aloe vera induces phenotypic and functional maturation of immature dendritic cells", Int Immunopharmacol, 1, pp.1275~1284 (2001)
  18. Lee S, "Neuroprotective effects of Protaetia brevitarsis seulensis’ water extracts on trimethyltin-induced seizures and hippocampal neurogeneration", Int J Mol Sci, 22, pp.679~ (2021)
  19. "Effects of the intake of mealworm shake in anti-cancer patients" (2022)[URL]
  20. Ministry of Food and Drug, "Information of health functional foods, Food Safety Korea portal", ~ (2022)
  21. Park SK, "Acidic polysaccharides isolated from Phellinus linteus induce phenotypic and functional maturation of murine dendritic cells", Biochem Biophys Res Commun, 312, pp.449~458 (2003)
  22. Pugh N, "Characterization of Aloeride, a new high-molecular-weight polysaccharide from Aloe vera with potent immunostimulatory activity", J Agric Food Chem, 49, pp.1030~1034 (2001)
  23. Statistics Korea, "Health functional foods" (2022), Korean Statistical Information service
  24. Terés S, "Oleic acid content is responsible for the reduction in blood pressure induced by olive oil", Proc Natl Acad Sci USA, 105, pp.13811~13816 (2008)
  25. Turini ME, "Cyclooxygenase-2 : a therapeutic target", Annu Rev Med, 53, pp.35~57 (2002)
  26. 유보경, "흰점박이꽃무지 유충(Protaetia brevitarsis seulensis Larvae) 열수 추출물의 MAPKs 및 NF-κB 신호전달을 통한 대식세포 내 면역 활성 효과", 한국식품영양과학회지, 51(2), pp.100~106 (2022), 한국식품영양과학회
  27. Zielinski MR, "Fatigue, sleep, and autoimmune and related disorders", Front Immunol, 10, pp.1827~ (2019)

관련 논문AI추천

갈색거저리(Tenebrio Molitor)의 항산화능과 Raw 264.7 대식세포의 항염증 효과권오준, 김동인, 김현정, 안봉전, 유재묘, 장재윤, 조영제, 조용훈한국식품저장유통학회 · Food Science and Preservation2016 · 제23권 제6호Immunostimulatory activity of hydrolyzed and fermented Platycodon grandiflorum extract occurs via the MAPK and NF-κB signaling pathway in RAW 264.7 cellsJung Jae In, Kim Eun Ji, Kim So Mi, Kim Soyeon, Lee Hyun Sook, Lim Jihoon, Woo Moonjea한국영양학회 · Nutrition Research and Practice2022 · 제16권 제6호Immune-Enhancing Effects of Green Lettuce (Lactuca sativa L.) Extracts through the TLR4-MAPK/NF-κB Signaling Pathways in RAW264.7 Macrophage Cells서현주, 정진부한국자원식물학회 · 한국자원식물학회지2020 · 제33권 제3호Immunostimulatory Effects of Cordyceps militaris on Macrophages by the Enhanced Production of Cytokines through the Activation of NF-κB공현석, 권정학, 김경제, 신슬미, 이성원, 이성정, 조경해대한면역학회 · Immune Network2010 · 제10권 제2호Anti-inflammatory Effects of Various Mushrooms in LPS-stimulated RAW264.7 Cells김진효, 노형준, 박정용, 서경혜, 이은영, 이지연, 정미선, 한재구한국자원식물학회 · 한국자원식물학회지2018 · 제31권 제5호Immune Enhancement Effects of Neutral Lipids, Glycolipids, Phospholipids from Halocynthia aurantium Tunic on RAW264.7 MacrophagesJang A-yeong, Park Woo Jung, Rod-in Weerawan, Shin Il-shik한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2024 · 제34권 제2호

이 간행물의 다른 논문 (10건)