갈색거저리(Tenebrio Molitor)의 항산화능과 Raw 264.7 대식세포의 항염증 효과
Antioxidant capacity and Raw 264.7 macrophage anti-inflammatory effect of the Tenebrio Molitor
0
조회
0
다운로드
22
인용
0
참고문헌
키워드
초록
The purpose of this paper is to investigate potential anti-inflammatory and anti-oxidant effects of Tenebrio molitor. Macrophage cell response by outside stimulation leads expression of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and trigger expression of genes which are affected by inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), resulting in formation of inflammatory factors like nitric oxide (NO) and Prostaglandin E2 (PGE2). Cell viability was determined by MTT assay. In order to investigate anti-inflammatory agents, the inhibitory effects on the production of lipopolysaccharide (LPS)-induced NO in RAW 264.7 cells were examined. T. Molitor significantly decreased the production of NO in a dose-dependent manner, and also reduced the expression of iNOS, a COX-2 protein. As a result, the levels of protein such as PGE2, iNOS, COX-2 and MARKs were significantly reduced compared to non-treated group in T. Molitor water extract (TDW) treated group. Also, antioxidant effect of T. Molitor were investigated using DPPH, ABTS+ and superoxide anion radical scavenging activity tests in cell-free system. Antioxidant activity of T. molitor was found low in the DPPH radical scavenging test while high in the ABTS+ and superoxide anion radical scavenging activity tests. These results show that TDW could be an effective anti-pro-inflammatory and anti-oxidant agent.
Abstract
The purpose of this paper is to investigate potential anti-inflammatory and anti-oxidant effects of Tenebrio molitor. Macrophage cell response by outside stimulation leads expression of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and trigger expression of genes which are affected by inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), resulting in formation of inflammatory factors like nitric oxide (NO) and Prostaglandin E2 (PGE2). Cell viability was determined by MTT assay. In order to investigate anti-inflammatory agents, the inhibitory effects on the production of lipopolysaccharide (LPS)-induced NO in RAW 264.7 cells were examined. T. Molitor significantly decreased the production of NO in a dose-dependent manner, and also reduced the expression of iNOS, a COX-2 protein. As a result, the levels of protein such as PGE2, iNOS, COX-2 and MARKs were significantly reduced compared to non-treated group in T. Molitor water extract (TDW) treated group. Also, antioxidant effect of T. Molitor were investigated using DPPH, ABTS+ and superoxide anion radical scavenging activity tests in cell-free system. Antioxidant activity of T. molitor was found low in the DPPH radical scavenging test while high in the ABTS+ and superoxide anion radical scavenging activity tests. These results show that TDW could be an effective anti-pro-inflammatory and anti-oxidant agent.
참고문헌 (23)
- Wattenberg LW, "Chemoprevention of cancer", Cancer Res, 45, pp.1~8 (1985)
- Brieskorn CH, "The structure of carnosol", J Org Chem, 29, pp.2293~2298 (1964)
- Ding C, "Targeting IL-6 in the treatment of inflammatory and autoimmune diseases", Expert Opin Investig Drugs, 18, pp.1457~1466 (2009)
- 정진부, "Anti-Inflammatory Effects of Ethyl Acetate Fraction from Cnidium officinale Makino on LPS-Stimulated RAW 264.7 and THP-1 Cells", 한국자원식물학회지, 25(3), pp.299~307 (2012), 한국자원식물학회[DOI]
- Lee HJ, "In vitro anti-inflammatory and anti-oxidative effects of cinnamomum camphora extracts", J Ethnopharmacol, 103, pp.208~216 (2006)
- Kim EY, "Regulation of autoimmune inflammation by pro-inflammatory cytokines", Immunol Lett, 120, pp.1~5 (2008)
- Sims JE, "Interleukin-1 signaling occurs exclusively via the type I receptor", Proc Natl Acad Sci USA, 90, pp.6155~6159 (1993)
- Grossman RM, "Interleukin-6 is expressed in high levels in psoriatic skin and stimulates proliferation of cultured human keratinocytes", Proc Natl Acad Sci USA, 86, pp.6367~6371 (1989)
- Wilmer JL, "Chemical induction of interleukin-8, a proinflammatory chemokine, in human epidermal keratinocyte cultures and its relation to cytogenetic toxicity", Cell Biol Toxicol, 11, pp.37~50 (1995)
- 박두상, "곤충자원으로부터의 항동맥경화 활성물질 탐색", 생약학회지, 35(3), pp.233~238 (2004), 한국생약학회
- 이상한, "약용곤충추출물 라이브러리를 이용한 항산화 활성의 초고속 검색", 한국식품저장유통학회지, 12(5), pp.482~488 (2005), 한국식품저장유통학회
- 유정미, "국산 및 중국산 갈색거저리(Tenebrio molitor)의 영양성분 및 유해물질 비교분석", 한국식품영양과학회지, 42(2), pp.249~254 (2013), 한국식품영양과학회
- Jung SJ, "Antifungal Effect and activity spectrum of crude antifungal proteins from hemolymph of larvae of T. Molitor in Korea", J Korean Soc Food Sci Nutr, 23, pp.232~237 (1995)
- Kim HJ, "Drying method by antimicrobial activity and antioxidant effect of T. Molitor", KJAE symposium, pp.284~ (2013)
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radical Biology and Medicine, 26, pp.1231~1237 (1999)
- Stirpe F, "The regulation of rat liver xanthine oxidase. conversion in vitro of the enzyme activity from dehydrogenase(type D)to oxidase(type O)", J Biol Chem, 244, pp.3855~3863 (1969)
- Carmichael J, "Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing", Cancer Res, 47, pp.936~942 (1987)
- Bartholomew B., "A rapid method for the assay of nitrate in urine using the nitrate reductase enzyme of Escherichia coli", Food Chem Toxicol, 22, pp.541~543 (1984)
- Samak G, "Superoxide and hydroxyl radical scavenging actions of botanical extracts of wagatea spicata", Food Chem, 115, pp.631~634 (2009)
- Gülçin İ, "Antiradical and antioxidant activity of total anthocyanins from perilla pankinensis decne", J Ethnopharmacol, 101, pp.287~293 (2005)
- Gülçin İ, "Antioxidant activity of caffeic acid(3, 4-dihydroxycinnamic acid)", Toxicology, 217, pp.213~220 (2006)
- 정용면, "부위별 식용백합 추출물의 항산화 및 항균효과", 한국식품과학회지, 39(4), pp.452~457 (2007), 한국식품과학회