The pepsinolytic hydrolysate from Johnius belengerii frame inhibited LPS-stimulated production of pro-inflammatory mediators via the inactivating of JNK and NF-κB pathways in RAW 264.7 macrophages
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The objective of this study was to investigate the anti-inflammatory effects of the pepsinolytic hydrolysate from the fish frame, Johnius belengerii, on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The J. belengerii frame hydrolysate (JFH) significantly suppressed nitric oxide (NO) secretion on LPS-stimulated RAW264.7 macrophages. Moreover, the JFH markedly inhibited the levels of protein and mRNA expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Furthermore, the LPS-stimulated mRNA expression of pro-inflammatory cytokines, including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 was downregulated when cells were cultured with the JFH. The JFH significantly reduced the phosphorylation of c-Jun N-terminal kinase (JNK) and the translocation of nuclear factor-kappa B (NF-κB) in macrophages. As the result, the JFH has the potential antiinflammatory activity via blocking the JNK and NF-κB signal pathways.
Abstract
The objective of this study was to investigate the anti-inflammatory effects of the pepsinolytic hydrolysate from the fish frame, Johnius belengerii, on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The J. belengerii frame hydrolysate (JFH) significantly suppressed nitric oxide (NO) secretion on LPS-stimulated RAW264.7 macrophages. Moreover, the JFH markedly inhibited the levels of protein and mRNA expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Furthermore, the LPS-stimulated mRNA expression of pro-inflammatory cytokines, including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 was downregulated when cells were cultured with the JFH. The JFH significantly reduced the phosphorylation of c-Jun N-terminal kinase (JNK) and the translocation of nuclear factor-kappa B (NF-κB) in macrophages. As the result, the JFH has the potential antiinflammatory activity via blocking the JNK and NF-κB signal pathways.
참고문헌 (39)
- Ahn CB, "Purification and anti-inflammatory action of tripeptide from salmon pectoral fin byproduct protein hydrolysate", Food Chem, 168, pp.151~156 (2015)[DOI]
- Ahn CB, "Antioxidant and anti-inflammatory peptide fraction from salmon byproduct protein hydrolysates by peptic hydrolysis", Food Res Int, 49, pp.92~98 (2012)[DOI]
- Bonnefous C, "Discovery of inducible nitric oxide synthase (iNOS) inhibitor development candidate KD7332, part 1: identification of a novel, potent, and selective series of quinolinone iNOS dimerization inhibitors that rae orally active in roden pain models", J Med Chem, 52, pp.3047~3062 (2009)[DOI]
- Cattaneo F, "Anti-inflammatory and antioxidant activities, functional properties and mutagenicity studies of protein and protein hydrolysate obtained from Prosopis alba seed flour", Food Chem, 161, pp.391~399 (2014)[DOI]
- Chalamaiah M, "Fish protein hydrolysates: proximate composition, amino acid composition, antioxidant activities and applications: a review", Food Chem, 135, pp.3020~3038 (2012)[DOI]
- Choi YY, "The antiinflammatory potential of Cortex Phellodendron in vivo and in vitro: downregulation of NO and iNOS through suppression of <TEX>$NF-{\kappa}B$<", TEX> and MAPK activation, 2014, pp.19, 214~220
- del Carmen M-LM, "Lupine protein hydrolysates inhibit enzymes involved in the inflammatory pathway", Food Chem, 151, pp.141~147 (2014)[DOI]
- Dennler S, "c-Jun inhibits transforming growth factor <TEX>${\beta}$<", TEX>-mediated transcription by repressing Smad3 transcriptional activity, 2000, pp.275, 28858~28865
- Halldorsdottir SM, "Oxidative processes during enzymatic hydrolysis of cod protein and their influence on antioxidant and immunomodulating ability", Food Chem, 142, pp.201~209 (2014)[DOI]
- Holen E, "Hydrolyzed fish proteins modulates both inflammatory and antioxidant gene expression as well as protein expression in a co culture model of liver and head kidney cells isolated from Atlantic salmon (Salmo salar)", Fish Shellfish Immun, 54, pp.22~29 (2016)[DOI]
- Hwang JW, "Purification and characterization of a novel peptide with inhibitory effects on colitis induced mice by dextran sulfate sodium from enzymatic hydrolysates of Crassostrea gigas", Fish Shellfish Immun, 33, pp.993~999 (2012)[DOI]
- Ishihara K, "IL-6 in autoimmune disease and chronic inflammatory proliferative disease", Cytokine Growth Factor Rev, 13, pp.357~368 (2002)[DOI]
- Jeong DH, "Skipjack tuna (Katsuwonus pelamis) eyeball oil exerts an anti-inflammatory effect by inhibiting <TEX>$NF-{\kappa}B$<", TEX> and MAPK activation in LPS-induced RAW 264.7 cells and croton oil-treated mice, 2016, pp.40, 50~56
- Jovanovic DV, "IL-17 stimulates the production and expression of proinflammatory cytokines, <TEX>$IL-{\beta}$<", TEX> and <TEX>$TNF-{\alpha}$<, J Immunol, pp.1998, 160[DOI]
- Jung WK, "Calcium-binding peptide derived from pepsinolytic hydrolysates of hoki (Johnius belengerii) frame", Eur Food Res Technol, 224, pp.763~767 (2007)[DOI]
- Kim AR, "6,6′-bieckol suppresses inflammatory responses by down-regulating nuclear <TEX>$factor-{\kappa}B$<", TEX> activation via Akt, JNK, and p38 MAPK in LPS-stimulated microglial cells, 2016, pp.38, 244~252
- Kim EK, "Purification of a novel nitric oxide inhibitory peptide derived from enzymatic hydrolysates of Mytilus coruscus", Fish Shellfish Immunol, 34, pp.1416~1420 (2013)[DOI]
- Kim SY, "Purification and characterization of antioxidant peptide from hoki (Johnius belengerii) frame protein by gastrointestinal digestion", J Nutr Biochem, 18, pp.31~38 (2007)[DOI]
- 김연계, "Ethanolic Extract of Chondria crassicaulis Inhibits the Expression of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 in LPS-Stimulated RAW 264.7 Macrophages", Fisheries and Aquatic Sciences, 14(4), pp.275~282 (2011), 한국수산과학회
- Kim YS, "Anti-inflammatory action of high molecular weight Mytilus edulis hydrolysates fraction in LPS-induced RAW264. 7 macrophage via <TEX>$NF-{\kappa}B$<", TEX> and MAPK pathways, 2016, pp.202, 9~14
- 고석천, "Anti-inflammatory effect of enzymatic hydrolysates from Styela clava flesh tissue in lipopolysaccharide-stimulated RAW 264.7 macrophages and in vivo zebrafish model", Nutrition Research and Practice, 9(3), pp.219~226 (2015), 한국영양학회[DOI]
- Kobayashi EH, "Nrf2 suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription", Nat Commun, 7, pp.11624~ (2016)[DOI]
- Lee SJ, "Purification and characterization of a nitric oxide inhibitory peptide from Ruditapes philippinarum", Food Che Toxicol, 50, pp.1660~1666 (2012)[DOI]
- Ligumsky M, "Role of interleukin 1 in inflammatory bowel disease-enhanced production during active disease", Gut, 31, pp.686~689 (1990)[DOI]
- Morales-Medina R, "Functional and antioxidant properties of hydrolysates of sardine (S. pilchardus) and horse mackerel (T. mediterraneus) for the microencapsulation of fish oil by spraydrying", Food Chem, 194, pp.1208~1216 (2016)[DOI]
- Ngo DH, "Angiotensin-I converting enzyme inhibitory peptides from antihypertensive skate (Okamejei kenojei) skin gelatin hydrolysate in spontaneously hypertensive rats", Food Chem, 174, pp.37~43 (2015)[DOI]
- Nguyen MHT, "Beneficial effect of abalone intestine gastrointestinal digests on osteoblastic MG-63 cell differentiation", J Aquat Food Prod Technol, 23, pp.436~446 (2014)[DOI]
- Van-Tinh Nguyen, "Abalone Haliotis discus hannai Intestine Digests with Different Molecule Weights Inhibit MMP-2 and MMP-9 Expression in Human Fibrosarcoma Cells", Fisheries and Aquatic Sciences, 15(2), pp.137~143 (2012), 한국수산과학회[DOI]
- 오건우, "Biological activities and biomedical potential of sea cucumber (Stichopus japonicus): a review", Fisheries and Aquatic Sciences, 20(4), pp.1~17 (2017), 한국수산과학회
- 천충길, "The Antioxidant and Anti-inflammatory Effects of Abalone Intestine Digest, Haliotis discus hannai in RAW 264.7 Macrophages", Biotechnology and Bioprocess Engineering, 17(3), pp.475~484 (2012), 한국생물공학회[DOI]
- Reuter S, "Oxidative stress, inflammation, and cancer: how are they linked?", Free Radical Bio Med, 49, pp.1603~1616 (2010)[DOI]
- BoMi Ryu, "Anti-inflammatory effect of polyphenol-rich extract from the red alga Callophyllis japonica in lipopolysaccharide-induced RAW 264.7 macrophages", ALGAE, 29(4), pp.343~353 (2014), 한국조류학회I[DOI]
- Segovia J, "Kruppel-like factor 6 and inducible nitric oxide synthase regulates apoptosis during respiratory syncytial virus infection (P6198)", J Immunol, 190, pp.189~ (2013)
- Sung NY, "Antiinflammatory effect of sweetfish-derived protein and its enzymatic hydrolysate on LPS-induced RAW 264. 7 cells via inhibition of <TEX>$NF-{\kappa}B$<", TEX> transcription, 2012, pp.78, 381~390
- Tak PP, "<TEX>$NF-{\kappa}B$<", TEX>: a key role in inflammatory diseases, 2001, pp.107, 7~11
- Wehbi VL, "Molecular mechanisms for cAMP mediated immunoregulation in T cells-role of anchored protein kinase A signaling units", Front in Immunol, 7, pp.222~ (2016)
- Yeom M, "Xanthii fructus inhibits inflammatory responses in LPS-stimulated RAW 264.7 macrophages through suppressing <TEX>$NF-{\kappa}B$<", TEX> and JNK, J Ethnopharmacol, pp.2015, 176[DOI]
- 윤호동, "Anti-inflammatory Effects of Ethanolic Extracts from Codium fragile on LPS-Stimulated RAW 264.7 Macrophages via Nuclear Factor kappaB Inactivation", Fisheries and Aquatic Sciences, 14(4), pp.267~274 (2011), 한국수산과학회
- Yuan Z, "Koumine attenuates lipopolysaccaridestimulated inflammation in RAW264. 7 macrophages, coincidentally associated with inhibition of <TEX>$NF-{\kappa}B$<", TEX>, ERK and p38 pathways, 2016, pp.17, 430~
