공침법을 이용한 마그네슘이 첨가된 biphasic calcium phosphate의 적합성 평가
Suitability evaluation of magnesium substituted biphasic calcium phosphates prepared by coprecipitation method
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초록
Mg-BCP(Mg Substituted BCP)를 제조하기 위하여 Ca(NO3)2·4H2O(Katayama chemical, Japan)과 (NH4)2·HPO4(Junsei chemical, Japan), Mg(NO3)2·6H2O(Junsei chemical, Japan)를 출발물질로 공침법(co-precipitation process)을 이용하여합성하였다. 제조된 분말의 마그네슘의 첨가에 따른 분말의 분광학적 특성은 FT-IR(MAGNA-IR 560, Nicolet)을 통하여 분석하였으며, SEM(S-4200, Hitachi)을 통하여 미세구조를 분석하였다. XRD 회절피크의 면적적분강도를 Integral Analysis (Rigaku, Japan)를 이용하여 HAp와 β-TCP의 비율을 확인하였다. 1.0 wt% 마그네슘이 첨가된 BCP 샘플의 경우 Hank’s solution에서의 2주 침적 후 표면에 침상의 HAp로 추정되는 결정이 성장하였다. 이러한 미세구조의 변화는 생활성을 가지는 마그네슘의 첨가가 BCP 조직 내에서 표면의 활성을 증가시켜 결정의 성장을 촉진시킨 것으로 판단된다.
Abstract
Magnesium-substituted BCP (biphasic calcium phosphate) powders were prepared by incorporating small amounts of magnesium into the structure of different hydroxyapatite (HAp)/β-tricalcium phosphate (β-TCP) ratios through coprecipitation method. A series of magnesium substitutions ranging from 0, 0.5, and 1.0 wt%, which are comparable to the measured magnesium contents, were performed. The obtained powders were characterized by the following analytical techniques: X-ray diffraction analysis (XRD), Thermo Gravimetric Analyzer (TGA) and Fourier transform infrared spectroscopy (FT-IR). The results have shown that substitution of magnesium in the calcium-deficient apatites resulted in the formation of biphasic mixtures of different HAP/β-TCP ratios after heating above 1000oC. The 1.0 wt% magnesiumsubstituted-BCP were soaked in Hank's solutions after 2 weeks to observe the morphology of the biocement, especially needle-like hydroxyapatite crystals and to estimate the length and diameter of nanoneedle crystals.
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