Si 및 Mg 이온이 교환된 biphasic calcium phosphate 분말의 생체활성 거동
Bioactivity behavior of Si and Mg ion-substituted biphasic calcium phosphate powders
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이온이 첨가된 BCP(biphasic calcium phosphate) 분말을 제조하기 위하여 Ca(NO3)2 · 4H2O, (NH4)2HPO4를 출발물질로 공침법(co-precipitation process)을 이용하여 합성하였다. Si-BCP 합성을 위하여 TEOS를 Mg-BCP 합성을 위해서는Mg(NO3)2 · 6H2O를 사용하였다. 합성된 분말의 열처리 후 결정상을 분석한 결과 HAp와 β-TCP가 혼재된 상을 관찰할 수있었다. 분광학적 분석에서도 인산칼슘의 PO43−와 OH− 그룹에 해당하는 밴드도 관찰되었다. 이온이 첨가된 BCP 분말의 생체활성 거동을 평가하기 위하여 Hanks' Balanced Salt Solution(HBSS)에 침적시켜 시간에 따라 형상의 변화 및 결정상을 분석한 결과, 두 샘플 모두에서 빠른 표면 활성을 나타내었다. 세포 성장률 평가에서도 대조군에 비하여 세포성장률이 우수함을 관찰하였다.
Abstract
The co-precipitation technique has been applied to synthesize biphasic calcium phosphate (BCP). Ca(NO3)2 · 4H2O,(NH4)2HPO4, TEOS and Mg(NO3)2 · 6H2O as the starting materials was used. After the heat treatment of powder crystalline phases HAp and β-TCP analysis showed a mixed phase. The overall spectra appear to have mainly two modes corresponding to characteristic PO43− and OH− groups. After immersion in Hanks’ Balanced Salt Solution (HBSS) for 1week a precipitation started to be formed with individual small granules on the specimen surface. An MTT assay indicated that ionic substituted BCP powders had no cytotoxic effects on MG-63 cells, and that they have good biocompatibility.
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