공침법으로 합성된 biphasic calcium phosphate 분말의 생체활성 거동
Bioactivity behavior of biphasic calcium phosphate powders prepared by co-precipitation method
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초록
BCP(biphasic calcium phosphate) 분말을 제조하기 위하여 Ca(NO3)2 · 4H2O, (NH4)2HPO4를 출발 물질로 공침법(co-precipitation process)을 이용하여 합성하였다. 합성된 분말의 열처리 전 후의 HAp(Hydroxyapatite)/β-TCP(β-Tricalcium phosphate) 결정상 비율 및 분광학적 특성을 XRD 및 FT-IR를 이용하여 분석하였다. BCP 분말의 생체활성 거동을 평가하기 위하여 Hanks’ Balanced Salt Solution(HBSS)에 침적시켜 시간에 따라 형상, 이온농도의 변화 및 결정상을 분석한 결과낮은 결정화도의 HAp가 생성됨을 확인하였다. BCP분말의 세포독성 평가에서도 대조군에 비하여 세포성장률이 우수함을관찰하였다.
Abstract
The co-precipitation technique has been applied to synthesize biphasic calcium phosphate (BCP). Ca(NO3)2 · 4H2O and (NH4)2HPO4 as the starting materials was used. X-ray diffraction (XRD) and Fourier transformed infrared (FT-IR)spectroscopy were used to characterize the structure of as-synthesized and calcined BCP powders. After immersion in Hanks’ Balanced Salt Solution (HBSS), for 1 week a precipitation started to be formed with individual small granules on the specimen surface. An MTT assay indicated that BCP powders have no cytotoxic effects on MG-63 cells, and that they have good biocompatibility.
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