본문 바로가기
KERD
논문KCI 등재

상백피 추출물의 피부상재균에 대한 항균활성과 항산화 효과

Antioxidative and Antimicrobial Activities of Morus alba L. Extracts against Human Skin Pathogens

2

조회

0

다운로드

12

인용

0

참고문헌

키워드

주제분류미용

초록

This study was performed to investigate the antioxidative activities and antimicrobial effect of Morus alba L. extracts against human skin pathogens. The antioxidant activities of extracts was used the method of DPPH radical and nitrite scavenging assay, total polyphenolics and flavonoid contents. The extracts were prepared by ethanol extraction method. Their antimicrobial activity against Propionibacterium acnes, Staphylococcus epidermidis, S. aureus, Pityrosporum ovale, Malassezia furfur, Candida albicans examined through the paper disc agar diffusion assay and minimum inhibitiory concentration(MIC). Among the 6 pathogens tested, the inhibition zones were more than 6 ㎜ in diameter against S. aureus, P. ovale, M. furfur and >5 ㎜ for S. epidermidis, P. acnes, C. albicans. MICs of human skin pathogens obtained by broth MIC dilution method were 12.5 ㎍/㎖ for S. epidermidis and 6.25 ㎍/㎖ for S. aureus, P. acnes, M. furfur and 3.1 ㎍/㎖ for P. ovale, C. albicans. The antioxidant activities of ethanol extracts of M. alba root bark were 71.85% in the DPPH assay at 400 ㎍/㎖. The contents of total phenolics(119.5 ㎍/㎖) and flavonoids(68.99 ㎍/㎖). Nitric oxide radical inhibition of showed 60.9% at 400 ㎍/㎖. These results confirmed that M. alba root bark has great potential the be a cosmeceutical ingredient with a natural antioxidant and antibiotics against human skin pathogens.

Abstract

This study was performed to investigate the antioxidative activities and antimicrobial effect of Morus alba L. extracts against human skin pathogens. The antioxidant activities of extracts was used the method of DPPH radical and nitrite scavenging assay, total polyphenolics and flavonoid contents. The extracts were prepared by ethanol extraction method. Their antimicrobial activity against Propionibacterium acnes, Staphylococcus epidermidis, S. aureus, Pityrosporum ovale, Malassezia furfur, Candida albicans examined through the paper disc agar diffusion assay and minimum inhibitiory concentration(MIC). Among the 6 pathogens tested, the inhibition zones were more than 6 ㎜ in diameter against S. aureus, P. ovale, M. furfur and >5 ㎜ for S. epidermidis, P. acnes, C. albicans. MICs of human skin pathogens obtained by broth MIC dilution method were 12.5 ㎍/㎖ for S. epidermidis and 6.25 ㎍/㎖ for S. aureus, P. acnes, M. furfur and 3.1 ㎍/㎖ for P. ovale, C. albicans. The antioxidant activities of ethanol extracts of M. alba root bark were 71.85% in the DPPH assay at 400 ㎍/㎖. The contents of total phenolics(119.5 ㎍/㎖) and flavonoids(68.99 ㎍/㎖). Nitric oxide radical inhibition of showed 60.9% at 400 ㎍/㎖. These results confirmed that M. alba root bark has great potential the be a cosmeceutical ingredient with a natural antioxidant and antibiotics against human skin pathogens.

참고문헌 (39)

  1. 정현아, "호장근 추출물이 피부질환 및 피부미용에 미치는 영향", 박사, pp.2008
  2. Trejo-Skalli A, "Association of a transglutaminase-related antigen with intermediate filaments", Proc. Natl. Acad Sci, 92, pp.8940~8944 (1995)
  3. 유윤정, "구강미생물학", pp.155~166 (2001), 군자출판사
  4. 박수연, "수종의 한약추출물이 피부질환과 관련된 균주6종에 미치는 항균력 및 염증기전에 미치는 영향", 박사, pp.2003
  5. 대한 병리학회, "병리학", pp.71~104 (1995), 고문사
  6. 대한 피부과학회 교과서 편찬위원회, "피부과학", pp.1~25 (2001), 여문각
  7. Kearney JN, "The follicular distribution and abundance of resident bacteria on human Skin", J. Gen. Microbiol-801., 130(4), pp.797~801 (1984)
  8. 김나래, "천연물유래 여드름 치료제제의 항균활성 측정", 한국미생물·생명공학회지, 37(1), pp.80~84 (2009), 한국미생물·생명공학회
  9. 김영주, "Dryobalanops aromatica 유래 용뇌의 Malassezia furfur에 대한 항균 활성", 한국미생물·생명공학회지, 33(3), pp.236~239 (2005), 한국미생물·생명공학회
  10. 손호용, "피부 여드름 치료제 개발을 위한 천연물의 항균활성 검색", 한국미생물·생명공학회지, 34(3), pp.265~272 (2006), 한국미생물·생명공학회
  11. 박욱연, "상백피 추출물로부터 항균성 물질의 분리 정제", J.Fd Hyg .Safety, 10(4), pp.225~230 (1995)
  12. Ikeda T, "Function and skin depigmental activity of crude drugs", Fragrance J., 6, pp.56~ (1990)
  13. 정승원, "Tyrosinase 활성을 저해하는 식물체의 탐색", Korean J. Food Sci, Echnol, 27, pp.891~896 (1995)
  14. Ishitsu H, "Anti-picornavirus Flavone Ro 09-0179. Antimicrob", Agents Chenother, 22(4), pp.611~616 (1982)
  15. Park UY, "Investigation of Optimum Extracting Condition and Antimicrobial Activity of the Extract from the Root Bark of Mourus alba", J Fd Hyg. Safety, 10(3), pp.139~145 (1995)
  16. MoK JS, "Antibacterial Activity of Ethanol Extract of Root Bark of Morus alba against Selected fish Pathogenic Bacteria", J. of Aquaculture, 14(4), pp.221~226 (2001)
  17. 유종엽, "상백피 추출물과 차나무 기름 제제가 여드름과 호기성 피부 상재균에 미치는 효과", 대한피부과학회지, 41(9), pp.1136~1141 (2003), 대한피부과학회
  18. Lee HS, "Hair Growth effects of Mori Cortex Radicis Mixture on the hair of rat", Korean J. Seric. Sci, 42(2), pp.83~85 (2000)
  19. Davidson PM, "Method for testing the efficacy of food antimicrobials", Food technol, 43, pp.148~155 (1989)
  20. Amster D, "Susceptibility testing of antimicrobial in liquid media, antibiotics in alboratory medicine. 4th ed", pp.52~111 (1996), Williams and Wikins
  21. Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
  22. Gutfinger T, "Polyphenols in olive olis", J Am Oil Chem Soc, 58, pp.966~968 (1981)
  23. Swaim T, "The phenolic constituents of Prunus domestica. The quantitative analysis of phenolic constituents", J. sci Food Agric, 10(2), pp.83~88 (1959)
  24. Gray JI, "Inhibition of N-nitrosamine formation in model food system", J. Food Sci, 40(5), pp.981~982 (1975)
  25. 이지희, "한국산 새빨간검둥이 추출물의 피부세균에 대한 항균활성", 한국수산과학회지, 39(3), pp.292~296 (2006), 한국수산과학회
  26. Adachi, Y, "The effect of antibiotics on the production of superantigen from Staphylococcus isolated from atopic dermatitis", J. Dermatol. Sci, 28, pp.76~83 (2002)
  27. Mi-Sun Kang, "Isolation and Identification of Lactic Acid Bacteria Inhibiting the Proliferation of Propionibacterium acnes and Staphylococcus epidermidis", Journal of Bacteriology and Virology, 39(1), pp.11~19 (2009), 대한미생물학회
  28. 이경숙, "여드름병변에서 채취한 Propionibacterium acnes의 분리, 동정 및 생화학적 특성 연구", 한국의류산업학회지, 8(5), pp.571~576 (2006), 한국의류산업학회
  29. 석귀덕, "새삼(Cuscuta japonica Choisy)및 실새삼(C. australis R.Be) 추출물의 여드름 유발균 Propionibacterium acnes 증식 억제 효과", 생약학회지, 35(4), pp.375~379 (2004), 한국생약학회
  30. 이숙경, "무좀균과 비듬균에 대한 대나무 기름의 항균효과", J. FD Hyg Safety, 18(3), pp.113~117 (2003)
  31. 장소영, "식물 추출물의 Pityrosporum ovale 및 Candida albicans에 대한 항진균 활성", 생약학회지, 34(4), pp.303~307 (2003), 한국생약학회
  32. 김미라, "식품위해성 미생물에 대한 대황(Rheum tanguticum)메탄올 추출물의 항균활성 및 성분분석", 한국식품조리과학회지, 19(4), pp.470~476 (2003), 한국식품조리과학회
  33. 이양숙, "구릿대 잎 추출물의 항산화 효과 및 생리활성", 한국식품저장유통학회지, 14(1), pp.78~86 (2007), 한국식품저장유통학회
  34. Salah N, "Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain-breaking antioxidants", Archives of Biochemistry and Biophysics, 322, pp.339~346 (1995)
  35. 지선옥, "상백피 추출물의 항산화 활성 및 미백효과", 한국자원식물학회지, 22(2), pp.145~151 (2009), 한국자원식물학회
  36. Ding, A. H, "Release of reactive nitrogen intermediates and active oxygen intermediates from mouse", Eur. J. Biochem, 187, pp.131~141 (1988)
  37. 송진욱, "목향 추출물의 항산화 및 항암활성", 한국환경보건학회지, 34(1), pp.55~61 (2008), 한국환경보건학회
  38. 고명수, "조릿대 줄기와 잎의 화학성분과 그 추출물의 항산화 및 항균활성", 한국식품저장유통학회지, 15(1), pp.125~132 (2008), 한국식품저장유통학회
  39. 임애경, "포공영의 열수 및 에탄올 추출물의 기능적 생리활성", 한국식품영양과학회지, 37(10), pp.1231~1237 (2008), 한국식품영양과학회

관련 논문AI추천

이 간행물의 다른 논문 (14건)