상백피 추출물의 항산화 활성 및 미백효과
Antioxidant Activities and Whitening Effect of the Mulberry (Morus alba L.) Root Bark Extracts
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초록
본 연구는 상백피 추출물의 화장품 약리활성인 항산화 활성과 미백 효과를 탐색하기 위해 열수추출물과 에탄올추 출물을 사용하여 전자공여능, ABTS, xanthine oxidase 저해활성, nitric oxide radical 소거능 및 tyrosinase 저 해 활성을 측정하였다. 상백피 추출물의 전자공여능 측정결과 1,000 μg/ml에서 열수추출물은 65.8%, 에탄올추출물은 87%의 DPPH radical 소거능을 보였다. ABTS 라디칼 소거능은 1,000 μg/ml에서 열 수추출물은 89.3%, 에탄올추출물은 77.1%로 높은 소거능 을 보였다. Xanthine oxide 저해활성은 1,000 μg/ml에서 열수추출물은 100%, 에탄올추출물은 96.2%를 보여 높은 항산화 효과를 나타내었다. NO 소거능은 500 μg/ml에서 열수추출물은 43.5%, 에탄올추출물은 53%를 나타내었는 데 이는 같은 농도에서 대조구인 BHA의 43.8%와 유사하 였다. Tyrosinase 저해활성은 1,000 μg/ml에서 열수추출 물은 79.6%, 에탄올추출물은 93.5%로 나타나 대조구인 비 타민 C와 유사한 활성을 보여 미백효과가 우수하였다. 모 든 실험에서 활성정도가 시료 농도가 증가함에 따라 유의 적으로 증가하는 경향이었으며, ABTS 저해활성을 제외하 고 대부분의 실험에서 에탄올추출물이 열수추출물에 비해 다소 높은 활성을 보였다.
Abstract
This study was performed to investigate the antioxidant activities and the whitening effect of mulberry (Morus alba L.) root bark extracts. The antioxidant activities of water and 70% ethanol extracts of mulberry root bark were 65.8% and 87.0% in the DPPH assay at 1,000 ㎍/ml ; 89.3% and 77.1% in the ABTS assay at 1,000 ㎍/ml. Xanthine oxidase inhibition of water and 70% ethanol extracts were 100% and 96.2% at 1,000 ㎍/ml. Nitric oxide radical inhibition of water and 70% ethanol extracts showed 43.5% and 53.0% at 500 ㎍/ml, and it was similar to the BHA effect(43.8%). Tyrosinase inhibitory activities of water and 70% ethanol extracts were 79.6% and 93.5% at 1,000 ㎍/ml. These results confirm that mulberry root bark has the great potential to be a cosmeceutical ingredient with a natural antioxidant and a skin-whitening effect. Key words : Morus alba L. DPPH, ABTS, Nitric oxide, Xanthine oxidase, Tyrosinase Abstract This study was performed to investigate the antioxidant activities and the whitening effect of mulberry (Morus alba L.) root bark extracts. The antioxidant activities of water and 70% ethanol extracts of mulberry root bark were 65.8% and 87.0% in the DPPH assay at 1,000 ㎍/ml ; 89.3% and 77.1% in the ABTS assay at 1,000 ㎍/ml. Xanthine oxidase inhibition of water and 70% ethanol extracts were 100% and 96.2% at 1,000 ㎍/ml. Nitric oxide radical inhibition of water and 70% ethanol extracts showed 43.5% and 53.0% at 500 ㎍/ml, and it was similar to the BHA effect(43.8%). Tyrosinase inhibitory activities of water and 70% ethanol extracts were 79.6% and 93.5% at 1,000 ㎍/ml. These results confirm that mulberry root bark has the great potential to be a cosmeceutical ingredient with a natural antioxidant and a skin-whitening effect.
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