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防己 추출물이 LPS로 유도된 Raw 264.7 cell에서의 TNF-α, IL-1β, IL-6 및 Nitric Oxide production에 미치는 영향

Inhibitory Effect of Stephanniae Tetrandrae Radix Extract on TNF-α, IL-1β, IL-6 and Nitric Oxide Production in Lipopolysaccharide - Activated RAW 264.7 Cells

김상찬|이종록|변성희|신상우|권영규|김대희
한의병리학회 (The Society of Pathology in Korean Medicine)|2006.08|동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)|제20권 제4호|pp. 902-908

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참고문헌

주제분류한의학

초록

Abstract

Tetrandra is the root of Stephania tetrandra S. Moore (family Menispermaceae), or of Aristolochia frangchi Wu (family Aristolochiaceae). It is a bitter-flavored and cold-property herb acting on the urinary bladder, kidney and spleen meridiands. Known biological effects of this herb are expelling wind to relieve pain and inducing diuresis to alleviate edema. This herb also has anti-inflammatory and anti-hypersensitivity actions. Recent studies have shown that Stephanniae Tetrandrae Radix has antimicrobial effects, namely, a protective effect on acute renal failure induce by gentamicin sulfate and a suppressive effect against clostridium perfringes. However, there is a lack of studies concerning the immunological activities of this herb. The present study was conducted to evaluate the immunological activities of Stephanniae Tetrandrae Radix on the regulatory mechanisms of cytokines and nitric oxide (NO) in Raw 264.7 cells. Cell viability was measured by MTT assay after the treatment of Stephanniae Tetrandrae Radix extract (STRE) and NO production was monitored by measuring the nitrite content in culture medium. COX-2 and iNOS were determined by immunoblot analysis, and levels of cytokine were analyzed by sandwich immunoassays. Results provided evidences that STRE inhibited the production of nitrite and nitrate (NO), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in Raw 264.7 cells activated with lipopolysaccharide (LPS). These findings showed that STRE could produce some anti-inflammatory effects which might play a role in adjunctive therapy in Gram-negative bacterial infections.

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