대식세포주 RAW264.7 세포에서 Curcumin의 Lipopolysaccharide 에 의한 Nitric Oxide 생성 억제 효과
Inhibitory Effect of Curcumin on Nitric Oxide Production in Lipopolysaccharide-Stimulated RAW264.7 Cells and Its Suppressive Mechanism
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Abstract
ABSTRACT : Curcumin, a polyphenolic antioxidant purified from turmeric, has been known to possess various biological activities such as anti-oxidative, anti-inflammatory and anti-cancer effects. In this study, we have explored anti-inflammatory effect of curcumin using Gram (−) bacterium-derived endotoxin (lipopolysaccharide: LPS) and macrophage cell line RAW264.7. Curcumin suppressed NO production in LPS-activated RAW264.7 cells in a dose-dependent manner. Curcumin also blocked the activation of NF-κB but not AP-1 according to luciferase assay. Furthermore, this compound suppressed the phosphorylation of a series of intracellular signaling components such as Src, JAK-2, Akt, IKK and IκBα under LPS stimulation in a time dependent manner. Therefore, our data suggest that curcumin was able to protect the host from Gram (−) bacterial-infection-mediated inflammatory symptoms.
참고문헌 (22)
- "Curcumin (diferuloylmethane) inhibits receptor activator of NF-kappa B ligand-induced NF-kappa B activation in osteoclast precursors and suppresses osteoclastogenesis", 173, pp.5940~5947 (2004)
- "Regulation of major histocompatibility complex class II antigens on human alveolar macrophages by granulocyte-macrophage colony-stimulating factor in the presence of glucocorticoids", 98, pp.104~110 (1999)
- "NF-kappaB, a pivotal transcription factor in silica-induced diseases", 234-235, pp.169~176 (2002)
- "In vitro antiinflammatory effects of cynaropicrin, a sesquiterpene lactone, from Saussurea lappa", 398, pp.399~407 (2000)
- "Identification of cooperative monomeric Brachyury sites conferring T-bet responsiveness to the proximal IFN-gamma promoter", 15, pp.1149~1160 (2003)
- "Curcumin suppresses growth and chemoresistance of human glioblastoma cells via AP-1 and NFkappaB transcription factors", 102, pp.522~538 (2007)
- "Blockade of NFkappaB activation and donation of nitric oxide: new treatment options in inflammatory bowel disease?", pp.37~41 (2002)
- "Biochemical and functional characterization of three activated macrophage populations", 80, pp.1298~1307 (2006)
- "Lipopolysaccharide-induced macrophage inflammatory response is regulated by SHIP", 173, pp.360~366 (2004)
- "Curcumin suppresses activation of NF-kappaB and AP-1 induced by phorbol ester in cultured human promyelocytic leukemia cells", 35, pp.337~342 (2002)
- "Identification and characterization of a new oncogene derived from the regulatory subunit of phosphoinositide 3-kinase", 17, pp.743~753 (1998)
- "Curcumin attenuates the release of pro-inflammatory cytokines in lipopolysaccharide-stimulated BV2 microglia", 28, pp.1645~1651 (2007)
- "Inhibitory effect of curcumin on nitric oxide production from lipopolysaccharide-activated primary microglia", 79, pp.2022~2031 (2006)
- "Curcumin suppresses Janus kinase-STAT inflammatory signaling through activation of Src homology 2 domain-containing tyrosine phosphatase 2 in brain microglia", 171, pp.6072~6079 (2003)
- "A pervasive role of ubiquitin conjugation in activation and termination of IkappaB kinase pathways", 6, pp.321~326 (2005)
- "Curcumin inhibits NF-kappaB activation and reduces the severity of experimental steatohepatitis in mice", 41, pp.926~934 (2004)
- "Molecular targets of curcumin", 595, pp.227~243 (2007)
- "Inflammatory cytokines and antigen presenting cell activation", 87, pp.109~121 (2002)
- "In vitro attenuation of nitric oxide production in C6 astrocyte cell culture by various dietary compounds", 218, pp.390~397 (1998)
- "Inflammatory cells and chronic obstructive pulmonary disease", 4, pp.607~618 (2005)
- "SRC family kinases: potential targets for the treatment of human cancer and leukemia", 9, pp.2043~2059 (2003)
- "Immunomodulatory effects of curcumin", 27, pp.485~497 (2005)