當歸芍藥散이 마우스 대식세포주의 NO 생성 및 사이토카인 유전자 발현에 미치는 영향
Effects of Dangguijakyak-san Extract on Nitric Oxide Production and Cytokine Gene Expression in RAW 264.7 Macrophages Cell
2
조회
0
다운로드
1
인용
0
참고문헌
초록
Abstract
The present study was conducted to evaluate the immunomodulatory effects of Dangguijakyak-san(當歸芍藥散). We investigated the effects of cell proliferation in mouse spleen cell and RAW 264.7 macrophages cells. Dangguijakyak-san enhanced mitogenic activity in the dose-response manner in mouse spleen cells and RAW 264.7 macrophages cells. In nitric oxide (NO) synthesis and iNOS mRNA expression by Dangguijakyak-san, Dangguijakyak- san alone had an effect on NO synthesis and iNOS mRNA expression in RAW 264.7 cells. NO production and iNOS mRNA expression which is excessively induced by LPS decreased after treatment of Dangguijakyak-san. The expressions of cytokine gene by Dangguijakyak-san investigated using reverse transcription polymerase chain reaction (RT-PCR). In RT-PCR, IL-1α, IL-1β and IL-6 mRNA expressions induced in Dangguijakyak-san-treated RAW 264.7 cells. These data indicate that 1) Dangguijakyak-san can modulate various immune response and 2) the immunomodulatory effects of Dangguijakyak-san may be, in part, associated with the regulation of NO synthesis, the expressions of these cytokine as well as the mitogenic effect on spleen cells and macrophages cells.
참고문헌 (20)
- "東醫方劑와 處方解說", pp.506~7 (1998), 尹用甲
- "산화질소의 화학과 응용", pp.52~63 (1999)
- "Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the with interferon-and lipopolysaccharide", pp.21~28 (2000)
- "Camptothecin suppresses nitric oxide", pp.7 625~635 (2001)
- "Polygonum tinctorium extract suppresses nitric oxide production by activated", koh j eth(k pharmacol 72), pp.141~150 (2000)
- "Suppression of inducible nitric oxide synthase expression in RAW 264 7", pp.301~309 (2000)
- "The aqueous extract of Rhodiola sachalinensis root enhances the expression", j eth(pharmacol 76), pp.7 119~123 (2001)
- "Inhibitory effects of butanol fraction of the aqueous extract of Forsythia koreana", j eth(pharmacol 73), pp.7 323~327 (2000)
- "Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated RAW264", pp.7 217~226 (2000)
- "Nitric oxide-scavenging and antioxidant effects of Uraria crinita root", pp.471~478 (2001)
- "IL-6 mRNA Expression in Mouse Peritoneal Macrophages and NIH3T3 Fibroblasts in Response to Candida albicans", pp.8~15 (2000)
- "augments the production of granulocyte-macrophage colony-stimulating factor from human peripheral blood mononuclear cells in vitro", int j immu(pharmacol), pp.595~598 (1991)
- "신수혈의 침자극과 황기약침이 실험용 생쥐의 면역활성물질인 cytokine의 IL-6 발현에 미치는 영향", pp.147~155 (1998)
- "황기가 면역세포의 기능에 미치는 영향", pp.115~128 (1998)
- "Differences in immunomodulating effects between wild and cultured Panax ginseng", pp.1672~1678 (1994)
- "인삼 사포닌이 인간면역계 사이토카인 유전자의 발현에 미치는 영향", pp.15~22 (1996)
- "마이토젠 및 림포카인이 마우스 비장 세포 임파구 및 사람 말초 혈액 임파구 증식 반응에 미치는 영향", pp.89~96 (1989)
- "Capsaicin 전처치가 마우스 대식세포의 기능에 미치는 영향", pp.39~49 (2000)
- "Membrane potential modulates release of tumor necrosis factor in lipopolysaccharide-stimulated mouse macrophages", pp.451~460 (1992), Romanin C
- "Stimulation of interleukin-6 and prostaglandin E2 secretion from peritoneal macrophages by polymers of albumin", pp.2853~2864 (1993)