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解毒四物湯의 抗炎效果 및 免疫反應에 關한 硏究

Anti-inflammatory Effects of Haedoksamultang in RAW 264.7 cells

조한백|김양호
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2008.05|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제21권 제2호|pp. 166-183

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참고문헌

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초록

Abstract

Purpose: This study was performed to determine anti-imflammatory effects of Haedoksamultang. Methods: In this study, I examined the effects of Haedoksamultang on the production of nitric oxide(NO), tumor necrosis factor-α(TNF-α), and interlukin-1β(IL-1β) as well as the expression of inducible NO synthase(iNOS), cyclooxygenase-2(COX-2), TNF-α, and IL-1β in RAW 264.7 cells. Haedoksamultang inhibited LPS-stimulated NO production. Western blotting and RT-PCR analysis showed that Haedoksamultang suppressed LPS-induced iNOS and COX-2 protein and mRNA expression in RAW 264.7 cells. Haedoksamultang also suppressed the expression and production of LPS-stimulated TNF-α and IL-1β in RAW 264.7 cells. Haedoksamultang inhibited NF-κB activation in LPS-treated RAW 264.7 cells. Moreover, this compound blocked IκB-α phosphorylation and nuclear translocation of the cytosolic NF-κB p65 subunit, which highly correlated with the production and expression of inflammatory mediators. Results: Haedoksamultang suppresses that inflammation-associated gene expression by blocking NF-κB activation. Conclusion: These results suggest that Haedoksamultang may be beneficial for treating inflammatory disease.

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