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괴화 추출물이 대식세포에서의 nitric oxide와 interleukin-6의 생성에 미치는 영향

Suppression of nitric oxide and interleukin-6 production by methanol extract of Sophorae Flos in macrophage cells

이지은|이주연|김종관|최점일|김성조
대한치주과학회 (The Korean Academy Of Periodontology)|2005.03|Journal of Periodontal & Implant Science|제35권 제1호|pp. 9-20

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참고문헌

주제분류치의학

초록

Abstract

Both nitric oxide (NO) and interleukin-6 (IL-6) have been thought to have a role in the pathogenesis of inflammatory periodontal disease as it does in other inflammatory diseases, and the inhibitors of NO and IL-6 production have been considered as potential anti-inflammatory agents. In this study, we evaluated methanol extract of Sophorae Flos for inhibition of NO and IL-6 production in Prevotella intermedia LPS-induced mouse macrophages RAW264.7 cells. Dried Sophorae Flos was sliced, and extracted with 100% methanol. LPS from P. intermedia ATCC 25611 was prepared by the standard hot phenol-water method. NO production was assayed by measuring the accumulation of nitrite in culture supernatants and IL-6 was measured using mouse IL-6 ELISA kit. Western blot analysis of iNOS and analysis of reverse transcription (RT)-PCR products were carried out. The methanol extract of Sophorae Flos concentration-dependently reduced the production of NO and the expression of iNOS protein and mRNA in RAW264.7 cells treated with P. intermedia LPS. Sophorae Flos also suppressed IL-6 production and the expression of IL-6 mRNA in RAW264.7 cells stimulated by P. intermedia LPS. The inhibition of NO and IL-6 production by Sophorae Flos may be useful in the therapy of inflammatory diseases such as periodontitis. This hypothesis, however, remains to be tested.

참고문헌 (38)

  1. "Nitric oxide:physiology,pathology,and pharmacology", 43, pp.109~142 (1991)
  2. "Nitric oxide synthases: roles, tolls and controls", pp.915~918 (1994)
  3. "and glucocorticoids regulate the expression of inducible nitric oxide synthase in hepatocytes", 90, pp.522~526 (1993)
  4. "Regulation of biosynthesis of nitric oxide", 269, pp.13725~13728 (1994)
  5. "Pathophysiological role of nitric oxide in rat experimental colitis", 19, pp.669~676 (1997)
  6. "The role of nitric oxide in the pathogenesis of spontaneous murine autoimmune disease increased nitric oxide production and nitric oxide synthase expression in MRL-lpr", lpr mice and reduction of spontaneous glomerulonephritis and arthritis by orally administered NG-monomethyl-L-arginine, 1994, 651~660
  7. "Nitric oxide synthesis is increased in periodontal disease", 33, pp.517~518 (1998)
  8. "LPS from Actinobacillus actinomycetemcomitans and production of nitric oxide in murine macrophages J774", 106, pp.576~581 (1998)
  9. "Nitric oxide production by a murine macrophage cell line stimulated with lipopolysaccharide from Actinobacillus actinomycetemcomitans", sosrose(w), pp.72~78 (2002)
  10. "Prevotella intermedia lipopolysaccharide stimulates release of nitric oxide by inducing expression of inducible nitric oxide synthase", pp.424~431 (2004)
  11. "Nitric oxide production and inducible nitric oxide synthase expression induced by Prevotella nigrescens lipopolysaccharide.FEMS Immunol Med Microbiol", 43, pp.51~58 (2005)
  12. "Nitric oxide synthesis and severity of human periodontal disease", 8, pp.254~260 (2002)
  13. "Expression of cytokines and inducible nitric oxide synthase in inflamed gingival tissue", 72, pp.590~597 (2001)
  14. "Nitric oxide synthase type-II is synthesized by human gingival tissue and cultured human gingival fibroblasts", 35, pp.194~200 (2000)
  15. "Inducible nitric oxide synthase expression in periodontitis", 35, pp.369~373 (2000)
  16. "The biology of interleukin-6", 74, pp.1~10 (1989)
  17. "Biological and clinical aspects of interleukin-6", pp.443~449 (1990)
  18. "IL-6 is produced by osteoblasts and induce bone resorption", 145, pp.3897~3903 (1990)
  19. "Assessment of interleukin-6 in the pathogenesis of periodontal disease", 65, pp.147~153 (1994)
  20. "Gingival interleukin-6 concentration following phase 1 therapy", 66, pp.667~672 (1990)
  21. "Production of cytokines by human gingival fibroblast", pp.265~276 (1991)
  22. "extraction with phenol-waterand further applications of the procedure Methods in carbohydrate chemistry", pp.83~91 (1965), Academic Press
  23. "A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples", 87, pp.206~210 (1978)
  24. nitrate in biological fluids, , 131~138
  25. "Novel method for studying mRNA phenotypes in single or small numbers of cells", 39, pp.1~11 (1989)
  26. "Antibiotic resistance of the subgingival microbiota following local tetracycline therapy", 7, pp.113~117 (1992)
  27. "Effects of supervised chlorhexidine mouthrinses in children", pp.101~111 (1982)
  28. "Does chlorhexidine have a place in the prophylaxis of dental diseases", pp.93~99 (1973)
  29. "Chemical plaque control and extrinsic tooth discoloration", 12, pp.345~350 (1985)
  30. "The effects of a topically-active nonsteroidal anti-inflammatory drug on ligature-induced periodontal disease in the squirrel monkey", 13, pp.139~144 (1986)
  31. "The effect of a topical non-steroidal anti-inflammatory drug on the development of experimental gingivitis in man", 16, pp.353~358 (1989)
  32. "Altering the progression of human alveolar bone loss with the non-steroidal anti-inflammatory drug flurbiprofen", 60, pp.485~490 (1989)
  33. "Topical flurbiprofen treatment of periodontitis in beagles Res 1988", pp.166~169 (1988)
  34. "Inhibition of alveolar bone loss in beagles with the NSAID naproxen", 26, pp.498~501 (1991)
  35. "Side effects of nonsteroidal anti-inflammatory drugs on the small and large intestine in humans", 104, pp.1832~1847 (1993)
  36. "Incidence of gastroduodenal ulcers associated with valecoxib compared with that of ibuprofen and diclofenac in patients with osteoarthritis", 14, pp.1101~1111 (2002)
  37. "생약학연구회", pp.317~321
  38. "생약학연구회", pp.317~321

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槐花 물 추출물의 항염증 효과구본순, 권강범, 김민선, 김성규, 김재효, 박경철, 박성주, 배기상, 서상완, 송호준, 윤명자, 윤승원, 전호성, 정원석, 조범연, 함경완한의병리학회 · 동의생리병리학회지2009 · 제23권 제6호防己 추출물이 LPS로 유도된 Raw 264.7 cell에서의 TNF-α, IL-1β, IL-6 및 Nitric Oxide production에 미치는 영향권영규, 김대희, 김상찬, 변성희, 신상우, 이종록한의병리학회 · 동의생리병리학회지2006 · 제20권 제4호LPS로 활성화된 Raw 264.7 Cells에서 고삼 뿌리 추출물의 항염증과 항산화 효과김범준, 김영희, 손동섭, 이동훈, 임윤영, 조대윤대한의진균학회 · 대한의진균학회지2010 · 제15권 제2호괴화 추출물 루틴의 소염작용: 쥐의 복강대식세포로부터 NO와 TNF-alpha 생산에 있어서 괴화 루틴에 의한 억제효과허억, 이무홍, 장성호, 정명수, 정재현한국약용작물학회 · 한국약용작물학회지2010 · 제18권 제2호金銀花 및 金銀花全草가 Raw 264.7 cell에서 LPS로 유도된 NO의 생성, iNOS, COX-2 및 cytokine에 미치는 영향권영규, 권택규, 김상찬, 김영우, 변성희, 변준석, 서성일, 신상우, 이동언, 이재령한의병리학회 · 동의생리병리학회지2005 · 제19권 제2호LPS로 활성화된 복강 대식세포에서신이 추출물의 염증성 사이토카인 및 NO 억제 효과김도윤, 문형철, 박성주, 정원석한의병리학회 · 동의생리병리학회지2007 · 제21권 제4호

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