LPS로 자극된 Raw 264.7 대식세포에서 한약재복합 추출물의 세포독성 및 항염증 효과
Anti-inflammatory effect and cytotoxicity of Mixed Extracts from Medicinal Herbs complex in the LPS-treated RAW 264.7 macrophages.
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Abstract
Amur Corktree, Paeonia lactiflora Pallas, Dictamni Radicis Cortex, nemarrhena asphodeloides Bunge, I nula helenium L have been used as a traditional medicine to treat inflammatory diseases in Korea. This study was designed to investigate the effects of Mixed Extracts from Korean Medicinal Herbs that inhi bits lipopolysaccharide(LPS)-induced inflammatory responses in Raw 264.7 macrophages. Raw 264.7 mac rophages were treated with various concentrations of the Medicinal Herbs complex (APDAL : Amur Co rktree, Paeonia lactiflora Pallas, Dictamni Radicis Cortex, nemarrhena asphodeloides Bunge, Inula hele nium L.). Nitric oxide PGE2, IL-6 inhibition effect of APDAL ethanol extracts was above 50% at a 50 μ g/mL concentration. The inhibitory effects against the expression of inducible NO syntase (iNOS) and c yclooxygenase-2 (COX-2) were 65.3%, 42.3% in APDAL at 50 μg/mL concentration. APDAL inhibited i nducible nitric oxide synthase (iNOS), COX-2 protein level on the LPS-indcued Raw 264.7 cells. In orde r to evaluate the effect of APDAL on a proliferation of RAW 264.7 cells, the MTT assay was carried o ut. results showed that APDAL has not cytotoxic at 50 μg/mL concentration.Thus, the anti-inflammator y reactions by APDAL were not affected by the cytotoxic effect. The results suggest the usefulness of developing functional materials using anti inflammatory.
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