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저강도 맥동성 초음파 적용이 관절연골세포에 미치는 영향

Effect of Low Intensity Pulsed Ultrasound in Rat Chondrocyte

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초록

Low intensity pulsed ultrasound(LIPUS) is known to accelerate bone regeneration, but the precise cellular signaling mechanism is still unclear. The purpose if this study was to determine the effect of LIPUS on the signaling mechanism of rat chondrocyte. In the explant culture condition, there was inhibition effect of 1 W/㎠ intensity LIPUS on chondrocytes proliferation but chondrocytes proliferation was increased at 0.25 W/㎠ intensity. In addition, western blot analysis of MAPKs showed that LIPUS increased ERK1/2 activity from the 10 min treatment of LIPUS. Hydrogen peroxide(H2O2), resulted in a time- and dose-dependent cell proliferation, which was largely attributed to apoptosis. H2O2 treatment caused marked sustained nucleus condensation in Hoechst stain. LIPUS and H2O2 activates phosphorylation of p-ERK1/2 and PD 98059(10-5M) blocked the effect of LIPUS and H2O2. Moreover, the synergistic phosphorylation of p44/42 MAPK by H2O2, LIPUS was selectively inhibited by PD 98059, ERK1/2 inhibitor. In order to determine whether the increase in cell proliferation caused by H2O2 and LIPUS could be explained by changes in the level of the prostaglandin E2. Our study demonstrated that LIPUS stimulate the cell proliferation via activated phosphorylation of ERK1/2 in condrocyte. LIPUS has anabolic effects on rat cartilage in explant cultures, indicating a potential important method for the treatment of osteoarthritic cartilarge.

Abstract

Low intensity pulsed ultrasound(LIPUS) is known to accelerate bone regeneration, but the precise cellular signaling mechanism is still unclear. The purpose if this study was to determine the effect of LIPUS on the signaling mechanism of rat chondrocyte. In the explant culture condition, there was inhibition effect of 1 W/㎠ intensity LIPUS on chondrocytes proliferation but chondrocytes proliferation was increased at 0.25 W/㎠ intensity. In addition, western blot analysis of MAPKs showed that LIPUS increased ERK1/2 activity from the 10 min treatment of LIPUS. Hydrogen peroxide(H2O2), resulted in a time- and dose-dependent cell proliferation, which was largely attributed to apoptosis. H2O2 treatment caused marked sustained nucleus condensation in Hoechst stain. LIPUS and H2O2 activates phosphorylation of p-ERK1/2 and PD 98059(10-5M) blocked the effect of LIPUS and H2O2. Moreover, the synergistic phosphorylation of p44/42 MAPK by H2O2, LIPUS was selectively inhibited by PD 98059, ERK1/2 inhibitor. In order to determine whether the increase in cell proliferation caused by H2O2 and LIPUS could be explained by changes in the level of the prostaglandin E2. Our study demonstrated that LIPUS stimulate the cell proliferation via activated phosphorylation of ERK1/2 in condrocyte. LIPUS has anabolic effects on rat cartilage in explant cultures, indicating a potential important method for the treatment of osteoarthritic cartilarge.

참고문헌 (35)

  1. Mitrovic, D., "Quintero M., Stankovic, A., Ryckewaert, A. Cell density of adult human femoral condylar articular cartilage. Joints with normal and fibrillated surfaces", Lab Invest, 49(3), pp.309~316 (1983)
  2. Gardner, D.L., "Problems and paradigms in joint pathology", J Anat, 184, pp.1749~1755 (1994)
  3. Hannan, M.T., "Bone mineral density and knee osteoarthritis in elderly men and women. The Framingham Study", Arthritis Rheum, 36(12), pp.1671~1680 (1993)
  4. Felson, D.T., "Osteoarthritis: new insights. Part 2: treatment approaches", Ann Intern Med, 133, pp.726~737 (2000)
  5. Lee, S.W., "TRAIL induces apoptosis of chondrocytes and influences the pathogenesis of experimentally induced rat osteoarthritis", Arthritis Rheum, 50(2), pp.534~542 (2004)
  6. Hashimoto, S., "Fas", Fas ligand expression and induction of apoptosis in chondrocytes, 1997, pp.40(10), 1749~1755
  7. Mow, V.C., "Some bioengineering considerations for tissue engineering of articular cartilage", Clin Orthop Relat Res, 367, pp.204~223 (1999)
  8. Chang, W., "Expression and signal transduction of calcium-sensing receptors in cartilage and bone", Endocrinology, 140(12), pp.5883~5893 (1999)
  9. Doan, N., "In vitro effects of therapeutic ultrasound on cell proliferation, protein synthesis, and cytokine production by human fibroblasts, osteoblasts, and monocytes", J. Oral Maxillofac Surg, 57(4), pp.409~419 (1999)
  10. Setton, L.A., "Mechanical behavior and biochemical composition of canine knee cartilage following periods of joint disuse and disuse with remobilization", Osteoarthritis Cartilage, 5(1), pp.1~16 (1997)
  11. Huang, M.H., "Effects of sonication on articular cartilage in experimental osteoarthritis", J. Rheumatol, 24(10)통권 : 1978, pp.1984~ (1997)
  12. Que, F.G., "Cell death by apoptosis: basic concepts and disease relevance for the gastroenterologist", Gastroenterology, 110(4), pp.1238~1243 (1996)
  13. Pelletier, J.P., "Selective inhibition of inducible nitric oxide synthesis reduces progression of experimental osteoarthritis in vivo", Arthritis Rheum, 43(6), pp.1290~1299 (2000)
  14. Raucci, A., "Activation of the ERK1", 2 and p38 mitogen-activated protein kinase pathways mediates fibroblast growth factor-induced growth arrest of chondrocytes, 2004, pp.279(3), 1747~1756
  15. Chang, L., "Mammalian MAP kinase signalling cascades", Nature, 410(6824), pp.37~40 (2001)
  16. Cano, E., "Parallel signal processing among mammalian MAPKs", Trends Biochem Sci, 20(3), pp.117~122 (1995)
  17. Wang, X., "The cellular response to oxidative stress: influences of mitogen-activated protein kinase signalling pathways on cell survival", Biochem J, 333, pp.291~300 (1998)
  18. Schortinghuis, J., "Ultrasound to stimulate early bone formation in a distraction gap: a double blind randomised clinical pilot trial in the edentulous mandible", Arch Oral Biol, 50(4), pp.411~240 (2005)
  19. Cook, S.D., "Kristiansen T.K. Acceleration of tibia and distal radious fracture healing in patients who smoke", Clin Orthop, 337, pp.198~207 (1997)
  20. Chapman, I.V., "Ultrasound- induced changes in rates of influx and efflux of potassium ions in rat thymocytes in vitro", Ultrasound Med Biol, 6(1), pp.47~58 (1980)
  21. Parvizi, J., "Low-intensity ultrasound stimulates proteoglycan synthesis in rat chondrocytes by increasing aggrcan gene expression", J. Orthop Res, 17(4), pp.488~494 (1997)
  22. Rubin, C., "The use of low-intensity ultrasound to accelerate the healing of fractures", J. Bone Joint Surg Am, 83A(9), pp.259~270 (2001)
  23. Naruse, K., "Distinct anabolic response of osteoblast to low-intensity pulsed ultrasound", Bone Miner Res, 18(2), pp.360~369 (2003)
  24. Yang, K.H., "Stimulation of fracture healing by low intensity pulsing ultrasound", The Journal of the Korean Society of Fracture, 11(2), pp.247~253 (1998)
  25. Kumar, A., "Distinct signaling pathways are activated in response to mechanical stress applied axially and transversely to skeletal muscle fibers", J. Biol Chem, 277(48), pp.46493~46503 (2002)
  26. Pouysségur, J., "Fidelity and spatio-temporal control in MAP kinase (ERKs) signalling", Biochem Pharmacol, 64(5-6), pp.755~763 (2002)
  27. Zhou, S., "Molecular mechanisms of low intensity pulsed ultrasound in human skin fibroblasts", J. Biol Chem, 279(52), pp.54463~54469 (2004)
  28. Chen, Y.J., "Pertussis toxin-sensitive Galphai protein and ERK-dependent pathways mediate ultrasound promotion of osteogenic transcription in human osteoblasts", FEBS Lett, 554(1-2), pp.154~158 (2003)
  29. Choi, B.H., "Mechanotransduction pathways of low-intensity ultrasound in C-28", I2 human chondrocyte cell line, 2007, pp.221(5), 527~535
  30. DiPietrantonio, A.M., "Activation of caspase 3 in HL-60 cells exposed to hydrogen peroxide", Biochem Biophys Res Commun, 225(2), pp.477~482 (1999)
  31. Xia, Z., "Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis", Science, 270(5240), pp.1326~1331 (1995)
  32. Stadheim, T.A., "Extracellular signal-regulated kinase (ERK) activity is required for TPA-mediated inhibition of drug-induced apoptosis", Biochem Biophys Res Commun, 245(1), pp.266~271 (1998)
  33. Asada, S., "Hydrogen peroxide induces apoptosis of chondrocytes; involvement of calcium ion and extracellular signal-regulated protein kinase", Inflamm Res, 50(1), pp.19~23 (2001)
  34. Reyes-Martin, P., "Cyclooxygenase-independent inhibition of H2O2-induced cell death by S-ketoprofen in renal cells", Pharmacol Res, 55(4), pp.295~302 (2007)
  35. Takahashi T., "Selective COX-2 inhibitor regulates the MAP kinase signaling pathway in human osteoarthritic chondrocytes after induction of nitric oxide", Int J. Mol Med, 15(2), pp.213~219 (2005)

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