저강도 초음파가 조골세포 활성에 미치는 영향
The Effect of Low Intensity Ultrasound on Osteoblast Activation
3
조회
0
다운로드
1
인용
0
참고문헌
초록
뼈의 형성과 재형성은 조골세포에 의한 골기질의 합성과 파골세포에 의한 재흡수에 의해 조절된다. 최근에는 골형성세포의 활성화를 통한 골조직의 재생 및 치유에 대한 연구가 활발히 이루어지고 있다. 골형성 세포의 활성화는 생물학적 자극뿐만 아니라 물리적 자극에 의해서도 영향을 받는 것으로 알려져 있다. 본 연구에서는 물리적 자극 가운데에서 연구가 거의 되지 않은 저강도 초음파가 골형성 세포의 활성에 미치는 영향을 조사하였다. 이를 위하여 우선 다양한 특성의 초음파를 사용함으로써 세포활성에 미치는 최적의 초음파 조건을 결정하였다. 본 연구 결과 전체 duty cycle에서 초음파 강도가 0.3W/cm2일 경우가 세포활성에 가장 적합하며 duty cycle 50%인 경우 최고의 세포수 증가율을 나타내었다. 이와 같이 골조직의 재생에 적합한 초음파의 특성을 결정하여 골절 및 골다공증의 치료에 초음파를 활용할 수 있을 것으로 사료된다.
Abstract
The bone formation and re-formation are regulated by two factors that are the synthesis of bone matrix by osteoblast and reabsorption by osteoclast. Recently, there are many studies about regeneration and healing of bone tissue by activation of osteoblast. In general, it is known that the activation of osteoblast is influenced by not only biological stimulus but physical stimulus. In this study, we verified that ostoeblast activation was influenced by low intensity ultrasound. Various ultrasonic properties were used to find out the most appropriate condition on cell activation. From this study, we could confirm that 0.3W/cm2 intensity of ultrasound was the most appropriate to cell activation over whole duty cycles and the increasing rate of cell was the highest at 50% duty cycle. Thus, it is expected that optimal ultrasonic characteristics on regeneration of bone matrix may be applied to fracture and osteoporosis healing.
참고문헌 (27)
- "Osteoprotegerin ligand: a regulator of immune responses and bone physiology"", 21(logy today), pp.497~ (2000)
- "Biologic effects of ultrasound" (1958)
- "Biological Effects of Ultrasound" (1985), Churchill Livingstone
- "The biomechanical effects of low-intensity ultrasound on healing tendons", pp.801~807 (1990)
- "Acceleration of tibia fracture-healing by non-invasive low intensity pulsed ultrasound", pp.26~34 (1994)
- "Ultrasound hyperthermia system for breast cancer treatment", 1873-1877 (1994)
- "The stimulation of bone growth by ultrasound", pp.153~159 (1983)
- "Enhancement of fracture healing by low intensity ultrasound", pp.216~229 (1998), Ryaby JP and Rubin C
- "The biomechanical effects of low-intensity ultrasound on healing tendons", 16, pp.801~807 (1990)
- "Effects of therapeutic ultrasound on the regeneration of skeletal myofibers after experimental muscle injury", 27, pp.54~59 (1999)
- "Effect of therapeutic ultrasound on endochondral ossification", 21, pp.121~127 (1995)
- "The effect of low-intensity pulsed ultrasound therapy on time to fracture healing", 166, pp.437~441 (2002)
- "Stimulation of bone repair by ultrasound", 2, pp.61~66 (1993)
- "Accleration of tibia and distal radius fracture healing in patients who smoke", 337, pp.198~207 (1997)
- "Accelerated healing of distal radial fractures with the use of specificlow intensity ultrasound"", 79A, pp.961~973 (1997)
- "The significance of membrane changes in the safe and dffective use of therpeutic and diagnostic ultrasound", din(m), pp.1543~1552 (1989)
- "In vitro effects of therapeutic ultrasound on the nucleus of human fibroblasts", 75, pp.629~634 (1995)
- "Stimulation of fracture healing by low intensity pulsing ultrasound The Journal of the Korean Society of Fracture", 11, pp.247~253 (1998)
- "A restriction point for control of normal animal cell proliferation" (1974)
- "Harrison's Principles of Internal Medicine" (1987), McGraw-Hill Book Co.
- "Relationship of cell growth to the regulation of tissue specific gene expression during osteoblast differentiation", pp.3111~3123 (1990)
- "Differential sensitivity of the cell life cycle", pp.141~156 (1963)
- "On the existence of a G0 phase in the cell cycle" (1970)
- "Cell division and the cell cycle in Finch Handbook of the Biology of Aging" (1977)
- "The growth fraction-A new concept applied to tumors" (1960)
- "Rate of chondroitin sulfate formation in wound healing"
- "Rate of chondroitin sulfate formation in wound healing", 165, pp.186~191, (1966)