저강도 초음파 조사 부위의 골 소실 억제 효과 - 골 밀도 및 형태학적 특성 평가
Suppression of Osteoporotic bone loss on the site to which low Intensity Ultrasound is Irradiated - In vivo test on BMD and Morphological Characteristics
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초록
Purpose : The aim of this study is to evaluate a possibility of clinical application for the effects of low intensity ultrasound stimulation (LIUS) in morphological characteristics (i.e., structure, bone mineral density) of bone on osteoporotic fracturesprevention. Materials and Methods : Eight virgin 14-week-old ICR mice (approximate weight 25g) were used and ovariectomized (OVX) to induce osteoporosis. Right tibia (US) for each mouse served as the LIUS (1.5MHz frequency, 1.0 kHz pulse repetition on frequency, 30mW/cm2㎠ intensity, 200㎲ pulse length, and stimulation for 20 minutes a day and 5 days a week over a 6-week period). Left tibia (CON) for each mouse served as the non-stimulated controls. Structural parameters and bone mineral density (g/㎤) on trabecular bone of tibiae were calculated and measured from images derived in-vivo micro computed tomography (micro-CT) at 0 week and after 6weeks. Results : The BV/TV and Tb.N in US group were significantly bigger than those in CON group. The Tb.Pf in US group, moreover, was significantly smaller than that in CON group (p＜0.05).For the others structural parameters and BMD, however, there were no significant difference between US group and CON group (p＞0.05). Conclusion : The LIUS might prevent bone loss and keep bone connectivity in osteoporotic bones. Therefore, the LIUS might prevent effectively the osteoporotic fractures in clinics.
Abstract
Purpose : The aim of this study is to evaluate a possibility of clinical application for the effects of low intensity ultrasound stimulation (LIUS) in morphological characteristics (i.e., structure, bone mineral density) of bone on osteoporotic fracturesprevention. Materials and Methods : Eight virgin 14-week-old ICR mice (approximate weight 25g) were used and ovariectomized (OVX) to induce osteoporosis. Right tibia (US) for each mouse served as the LIUS (1.5MHz frequency, 1.0 kHz pulse repetition on frequency, 30mW/cm2㎠ intensity, 200㎲ pulse length, and stimulation for 20 minutes a day and 5 days a week over a 6-week period). Left tibia (CON) for each mouse served as the non-stimulated controls. Structural parameters and bone mineral density (g/㎤) on trabecular bone of tibiae were calculated and measured from images derived in-vivo micro computed tomography (micro-CT) at 0 week and after 6weeks. Results : The BV/TV and Tb.N in US group were significantly bigger than those in CON group. The Tb.Pf in US group, moreover, was significantly smaller than that in CON group (p＜0.05).For the others structural parameters and BMD, however, there were no significant difference between US group and CON group (p＞0.05). Conclusion : The LIUS might prevent bone loss and keep bone connectivity in osteoporotic bones. Therefore, the LIUS might prevent effectively the osteoporotic fractures in clinics.
참고문헌 (11)
- Carvalho DCL, "The action of low-intensity pulsed ultrasound in bones of osteopenic rats", Artificial Organs, 28, pp.114~118 (2004)
- Clase L, "The enhancement of bone regeneration by ultrasound", Progress in Biophysics and Molecular Biology, 93, pp.384~398 (2007)
- Cowin S, "Bone mechanics handbook", pp.1~32 (2000), CRC press
- 고창용, "전신진동이 골다공증이 유발된 쥐 해면골에 미치는 영향- 골다공증 치료제 효과와 비교", 한국정밀공학회지, 25(5), pp.148~154 (2008), 한국정밀공학회
- 고창용, "Micro -computed tomography를 이용한 골다공증의새로운 형태학적 연구기법", 대한체질인류학회지, 18(4), pp.283~290 (2005), 대한체질인류학회
- 고창용, "난소 절제술을 시행한 흰쥐 등뼈의 해면골과 피질골의 구조적 특성 추적관찰 및 기계적 특성 분석-생체 내 미세단층촬영 시스템과 미세유한 요소법 적용-", 한국정밀공학회지, 24(1), pp.110~117 (2007), 한국정밀공학회
- Lacey JJ, "Menopausal hormone replacement therapy and risk of ovarian cancer", JAMA : the journal of the American Medical Association, 288, pp.334~341 (2002)
- Rubin C, "The Use of Low-Intensity Ultrasound to Accelerate the Healing of Fractures", The Journal of Bone and Joint Surgery, 83, pp.259~270 (2001)
- Schairer C, "Menopausal Estrogen and Estrogen-Progestin Replacement Therapy and Breast Cancer Risk", JAMA : the journal of the American Medical Association, 283, pp.485~491 (2000)
- "The Korean Society of Bone Metabolism: Physician`s guideline for osteoporosis", Seoheung, ~ (2007)
- Warden SJ, "Skeletal effects of low-intensity pulsed ultrasound on the ovariectomized rodent", Ultrasound in Medicine and Biology, 27, pp.989~998 (2001)