본문 바로가기
KERD
논문KCI 등재

우슬이 산화적 스트레스와 관련한 세포내 신호전달계에 미치는 영향

Effect of Achyrantis Radixs on Rat Chondrocyte Oxidative Stress and Its Signal Transduction

2

조회

0

다운로드

2

인용

0

참고문헌

키워드

주제분류한의학

초록

Archyranthes radix has had extensive therapeutic application, and there has been increasing interest in its biological effects. However, the biochemical effects of Archyranthes radix on chondrocyte oxidative stress have never been systematically investigated. Therefore, we investigated the effects of Acyranthes radix on role of MAPK signal transduction pathway on oxidative stress induced by hydrogen peroxide in rat articular chondrocytes. The statistically significant inhibitory action of Archyranthes radix on cell proliferation was observed at above 5 ㎍/㎖. Next, we examined the time-dependent effect of 5 ㎍/㎖ Archyranthes radix on cell proliferaion. Archyranthes radix significantly inhibited cell proliferation from 12 hr after treatment (P<0.05). H2O2, resulted in a time- and dose-dependent cell proliferation, which was largely attributed to oxidative damage. Acyranthes radix and H2O2 treatment caused marked sustained activation of phosphorylation of ERK1/2. Moreover, the synergistic phosphorylation of p44/42 MAPK by H2O2 and Archyranthes radix was selectively inhibited by PD 98059, a p44/42 MAPK inhibitor. In conclusion, these results are consistent with the hypothesis that under conditions of oxidative stress, the H2O2-induced inhibition of cell proliferation in the rat chondrocyte is mediated through a modulation of the Archyranthes radix signaling pathway, promoting further phosphorylation of p44/42 MAPK, indicating a potentially important role in cartilage repair and in the treatment of osteoarthritic cartilage.

Abstract

Archyranthes radix has had extensive therapeutic application, and there has been increasing interest in its biological effects. However, the biochemical effects of Archyranthes radix on chondrocyte oxidative stress have never been systematically investigated. Therefore, we investigated the effects of Acyranthes radix on role of MAPK signal transduction pathway on oxidative stress induced by hydrogen peroxide in rat articular chondrocytes. The statistically significant inhibitory action of Archyranthes radix on cell proliferation was observed at above 5 ㎍/㎖. Next, we examined the time-dependent effect of 5 ㎍/㎖ Archyranthes radix on cell proliferaion. Archyranthes radix significantly inhibited cell proliferation from 12 hr after treatment (P<0.05). H2O2, resulted in a time- and dose-dependent cell proliferation, which was largely attributed to oxidative damage. Acyranthes radix and H2O2 treatment caused marked sustained activation of phosphorylation of ERK1/2. Moreover, the synergistic phosphorylation of p44/42 MAPK by H2O2 and Archyranthes radix was selectively inhibited by PD 98059, a p44/42 MAPK inhibitor. In conclusion, these results are consistent with the hypothesis that under conditions of oxidative stress, the H2O2-induced inhibition of cell proliferation in the rat chondrocyte is mediated through a modulation of the Archyranthes radix signaling pathway, promoting further phosphorylation of p44/42 MAPK, indicating a potentially important role in cartilage repair and in the treatment of osteoarthritic cartilage.

참고문헌 (46)

  1. Archer, C.W, "Cellular aspects of the development of diarthodial joints and articular cartilage", J Anat, 184, pp.447~456 (1994)
  2. Mitrovic, D, "Cell density of adult human femoral condylar articular cartilage. Joints with normal and fibrillated surfaces", Lab Invest, 49(3), pp.309~316 (1983)
  3. Gardner, D.L, "Problems and paradigms in joint pathology", J Anat, 184, pp.1749~1755 (1994)
  4. McCored, J.M, "The evolution of free radicals and oxidative stress", Am J Med, 108, pp.652~659 (2000)
  5. Lee, W.C, "Role of ERK in hydrogen peroxide-induced cell death of human glioma cells", Neurochem Res, 30(2), pp.263~270 (2005)
  6. Galaris, D, "The role of oxidative stress in mechanisms of metal-induced carcinogenesis", Crit Rev Oncol Hematol, 42, pp.93~103 (2002)
  7. Balaban, R.S, "Mitochondria, oxidants, and aging", Cell, 120(4), pp.483~495 (2005)
  8. Galaris, D, "Oxidative stress in hepatic ischemia-reperfusion injury: the role of antioxidants and iron chelating compounds", Curr Pharm Des, 12, pp.2875~2890 (2006)
  9. Halliwell, B, "Oxidative stress and neurodegeneration: where are we now?", J Neurochem, 97, pp.1634~1658 (2006)
  10. Rhee, S.G, "Cell signaling: H2O2, a necessary evil for cell signaling", Science, 312(5782), pp.1882~1883 (2006)
  11. Stone, J.R, "Hydrogen peroxide: a signaling messenger", Antioxid Redox Signaling, 8, pp.243~270 (2006)
  12. Jin, N, "H2O2-induced egr-1, fra-1, and c-jun gene expression is mediated by tyrosine kinase in aortic smooth muscle cells", Free Radic Biol Med, 29, pp.736~746 (2000)
  13. Go, Y.M, "H2O2-dependent activation of GCLC-ARE4 reporter occurs by mitogen-activated protein kinase pathways without oxidation of cellular glutathione or thioredoxin-1", J Biol Chem, 279, pp.5837~5845 (2004)
  14. Vilhardt, F, "The phagocyte NADPH oxidase depends on cholesterol-enriched membrane microdomains for assembly", EMBO J, 23, pp.739~748 (2004)
  15. Rhee, S.G, "Intracellular messenger function of hydrogen peroxide and its regulation by peroxiredoxins", Curr Opin Cell Biol, 17(2), pp.183~189 (2005)
  16. Tenopoulou, M, "Role of compartmentalized redox-active iron in hydrogen peroxide-induced DNA damage and apoptosis", Biochem J, 387, pp.703~710 (2005)
  17. Geilen, C.C, "The mitogen-activated protein kinases system (MAP kinase cascade): its role in skin signal transduction", J Dermatol Sci, 12(3), pp.255~262 (1996)
  18. Chang, L, "Mammalian MAP kinase signalling cascades", Nature, 410통권 : 6824, pp.37~40 (2001)
  19. Cano, E, "Parallel signal processing among mammalian MAPKs", Trends Biochem Sci, 20(3), pp.117~122 (1995)
  20. Boulton, T.G, "ERKs: a family of protein-serine", threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF, 1991, pp.65(4), 663~675
  21. Khanna, A.K, "Hypolipidemic activity of Achyranthes aspera Linn in normal and triton induced hyperlipidamic rats", J Exp Biol, 30(2), pp.128~130 (1992)
  22. Desta, B, "Ethiopian traditional herbal drugs. Part Ⅱ: Antimicrobial activity of 63 medicinal plants", J Ethnopharmacol, 39(2), pp.129~139 (1993)
  23. Tian, G.Y, "Synthesis of Achyranthes bidentata polysaccharide sulfate and its antivirus activity", Yao Xue Xue Bao, 30, pp.107~111 (1995)
  24. Lu, T, "The research on analgestic and anti-inflammatory action of different processed products of Achyranthes bidentata", Zhong Yao Cae, 20, pp.507~509 (1997)
  25. Ratra, P.S, "Alkaloid in two species of Achyranthes at different stages of their growth", Trends Sci Life, 4, pp.81~85 (1979)
  26. Seshatri, V, "Structure of two new saponins from Achyranthes aspera", Indian J Chem Ser, 20, pp.773~775 (1981)
  27. Raman, M.H, "Studies on the antibacterial properties of Achyranthes aspera stem", Fitoterapia LⅫ, pp.92~93 (1996)
  28. Takemoto, T, "Studies on the constituents of Achyranthes radix. Ⅶ. The insect-moulting substances in Achyranthes and Cyathula genera "supplement"", Yakugaku Zasshi, 88(10), pp.1293~1297 (1968)
  29. Roehm, N.W, "An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT", J Immunol Methods, 142(2), pp.257~265 (1991)
  30. Bradford, M.M, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal Biochem, 72, pp.48~254 (1976)
  31. Gao, X.Y, "Determination of ecdysterone in Achyranthes bidentata Bl. and its activity promoting proliferation of osteoblast-like cells", Yao Xue Xue Bao, 35(11), pp.868~870 (2000)
  32. Halliwell, B, "Oxidative stress and neurodegeneration: where are we now?", J Neurochem, 97, pp.1634~1658 (2006)
  33. DiPietrantonio, A.M, "Activation of caspase 3 in HL-60 cells exposed to hydrogen peroxide", Biochem Biophys Res Commun, 255(2), pp.477~482 (1999)
  34. Lee, W.C, "Role of ERK in hydrogen peroxide-induced cell death of human glioma cells", Neurochem Res, 30(2), pp.263~270 (2005)
  35. Xia, Z, "Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis", Science, 270(5240), pp.1326~1331 (1995)
  36. Wang, X, "The cellular response to oxidative stress: influences of mitogen-activated protein kinase signalling pathways on cell survival", Biochem J, 15(333), pp.291~300 (1998)
  37. Stadheim, T.A, "Extracellular signal-regulated kinase (ERK) activity is required for TPA-mediated inhibition of drug-induced apoptosis", Biochem Biophys Res Commun, 245(1), pp.266~271 (1998)
  38. Asada, S, "Hydrogen peroxide induces apoptosis of chondrocytes; involvement of calcium ion and extracellular signal-regulated protein kinase", Inflamm Res, 50(1), pp.19~23 (2001)
  39. Ishikawa, Y, "Anti-apoptotic effect of quercetin: intervention in the JNK- and ERK-mediated apoptotic pathways", Kidney Int, 58(3), pp.1078~1087 (2000)
  40. Kang, H.J, "Roles of JNK-1 and p38 in selective induction of apoptosis by capsaicin in ras-transformed human breast epithelial cells", Int J Cancer, 103(4), pp.475~482 (2003)
  41. Jung, M.Y, "Chemopreventive allylthiopyridazine derivatives induce apoptosis in SK-Hep-1 hepatocarcinoma cells through a caspase-3-dependent mechanism", Eur J Cancer, 37(16), pp.2104~2110 (2001)
  42. Wattenberg, L.W, "Inhibition of carcinogenesis by minor anutrient constituents of the diet", Proc Nutr Soc, 49(2), pp.173~183 (1990)
  43. Kim, M.S, "Inhibition of invasion and induction of apoptosis by curcumin in H-ras-transformed MCF10A human breast epithelial cells", Arch Pharm Res, 24(4), pp.349~354 (2001)
  44. Kim, H.A, "Apoptotic chondrocyte death in human osteoarthritis", J Rheumatol, 27, pp.455~462 (2000)
  45. Takahashi, T, "Selective COX-2 inhibitor regulates the MAP kinase signaling pathway in human osteoarthritic chondrocytes after induction of nitric oxide", Int J Mol Med, 15(2), pp.213~219 (2005)
  46. Wang, H, "Apoptosis induced by NO via phosphorylation of p38 MAPK that stimulates NF-kappaB, p53 and caspase-3 activation in rabbit articular chondrocytes", Cell Biol Int, 31(9), pp.1027~1035 (2007)

관련 논문AI추천

저강도 맥동성 초음파 적용이 관절연골세포에 미치는 영향김계엽, 김은정한의병리학회 · 동의생리병리학회지2008 · 제22권 제5호Anti-osteoarthritis effects of Pomegranate, Eucommiae cortex and Achyranthis radix extracts on the primary cultured rat articular chondrocytes강수진, 구세광, 박기문, 박혜림, 성미선, 이영준, 최범락대한예방한의학회 · 대한예방한의학회지2017 · 제21권 제3호Concentration-dependent in vitro Anti-osteoarthritis Effects of Mixed Formula – Pomegranate Concentrate Powder: Eucommiae Cortex: Achyranthis Radix 5:4:1 (g/g) on the Primary Cultured Rat Articular Chondrocytes강수진, 구세광, 박기문, 박혜림, 성미선, 이영준, 최범락한의병리학회 · 동의생리병리학회지2019 · 제33권 제2호고농도 포도당에 노출된 마우스 신장상피세포에서 桑枝의 산화 스트레스 억제 효과신현철, 장수영대한한방내과학회 · 대한한방내과학회지2011 · 제32권 제1호Mouse의 신장상피세포에서 敗醬추출물이 산화 스트레스 및 NF-κB signaling에 미치는 영향김현영, 신현철, 장수영, 최규호대한한방내과학회 · 대한한방내과학회지2010 · 제31권 제1호Black rice extract protected HepG2 cells from oxidative stress-induced cell death via ERK1/2 and Akt activation김대일, 김영화, 성지혜, 윤재민, 이점식, 이준수, 정헌상, 최영민, 함현미한국영양학회 · Nutrition Research and Practice2014 · 제8권 제2호

이 간행물의 다른 논문 (26건)