미세전류자극 강도가 흰쥐의 골관절염 회복에 미치는 영향
The Effect of Microcurrent Stimulation Intensity on Osteoarthritis in Rat
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Osteoarthritis is a degenerative joint disease and is led to physical disability. Yet the development of effective disease-modifying treatments has lagged. In this study, I examined the effect of physical therapeutic intervention through microcurrent stimulation and attempt to find which degree of intensity, either 25 ㎂ or 500 ㎂ with a regular 5 pps pulse, is more effective in the osteoarthritis. Osteoarthritis was induced with a mixture of 2% carrageenan and 2% kaolin in 26 male Sprague-Dawley rats. The mixture (0.1 ㎖) was injected into the intra-articular capsule of knee joint once a week for three weeks. Five animals did not show degenerative changes by radiological findings and excluded in the following experiment. Osteoarthritic animals were randomly divided into 3 groups(n1, n2, n3=7/each): untreated, treated with 25 ㎂, treated with 500 ㎂. All experimental groups received microcurrent stimulation for four weeks (15 min/day, 5 days/week). The ethological inspection of foot print analysis on the walking corridor was accomplished every week. Histological preparations and immunohistochemical staining with insulin-like growth factor-1 were also done in the articular cartilages. All of these parameters were compared with those of osteoarthritic control group (n=7). The ethological inspection of foot print analysis revealed that changes of walking track (paw width) and stride length was significantly increased in both experimental groups. The better results were observed in experimental group treated with 25 ㎂ intensity without significance than group treated with 500 ㎂. Histological preparations disclosed that routine hyaline cartilage of articular surface were altered to fibrous cartilage in untreated group and experimental group treated with 500 ㎂ intensity. But a little changes were seen in experimental group treated with 25 ㎂ intensity. Immunolocalization of insulin-like growth factor-1 was simultaneously decreased according to the duration of osteoarthritis, and did not show significant difference among the groups. In this study discovered that the microcurrent stimulation, especially 25 ㎂ intensity, had a positive effect by the ethological inspection, histological and immunohistochemical stainings. These results suggest that microcurrent stimulation with low-intensity might be effective in the promotion of healing process for the osteoarthritis.
Abstract
Osteoarthritis is a degenerative joint disease and is led to physical disability. Yet the development of effective disease-modifying treatments has lagged. In this study, I examined the effect of physical therapeutic intervention through microcurrent stimulation and attempt to find which degree of intensity, either 25 ㎂ or 500 ㎂ with a regular 5 pps pulse, is more effective in the osteoarthritis. Osteoarthritis was induced with a mixture of 2% carrageenan and 2% kaolin in 26 male Sprague-Dawley rats. The mixture (0.1 ㎖) was injected into the intra-articular capsule of knee joint once a week for three weeks. Five animals did not show degenerative changes by radiological findings and excluded in the following experiment. Osteoarthritic animals were randomly divided into 3 groups(n1, n2, n3=7/each): untreated, treated with 25 ㎂, treated with 500 ㎂. All experimental groups received microcurrent stimulation for four weeks (15 min/day, 5 days/week). The ethological inspection of foot print analysis on the walking corridor was accomplished every week. Histological preparations and immunohistochemical staining with insulin-like growth factor-1 were also done in the articular cartilages. All of these parameters were compared with those of osteoarthritic control group (n=7). The ethological inspection of foot print analysis revealed that changes of walking track (paw width) and stride length was significantly increased in both experimental groups. The better results were observed in experimental group treated with 25 ㎂ intensity without significance than group treated with 500 ㎂. Histological preparations disclosed that routine hyaline cartilage of articular surface were altered to fibrous cartilage in untreated group and experimental group treated with 500 ㎂ intensity. But a little changes were seen in experimental group treated with 25 ㎂ intensity. Immunolocalization of insulin-like growth factor-1 was simultaneously decreased according to the duration of osteoarthritis, and did not show significant difference among the groups. In this study discovered that the microcurrent stimulation, especially 25 ㎂ intensity, had a positive effect by the ethological inspection, histological and immunohistochemical stainings. These results suggest that microcurrent stimulation with low-intensity might be effective in the promotion of healing process for the osteoarthritis.
참고문헌 (25)
- 강종호, "고정이 관절연골에 미치는 영향에 대한 연구", 대한물리치료학회지, 16(2), pp.1~16 (2004), 대한물리치료학회
- Bang HS, "The effect of microcurrent electrical stimulation on the articular cartilage rcovery in osteoarthritis", J Kor Soc Phys Med, 2(2), pp.151~159 (2007)
- Barker AT, "The glabrous epidermis of cavies contains a powerful battery", Am J Physiol, 242(3), pp.358~366 (1982)
- Bayat M, "Experimental wound healing using microamperage electrical stimulation in rabbits", J Rehabil Res Dev, 43(2), pp.219~226 (2006)
- Brenner SS, "Osteoarthritis of the knee: Clinical assessments and inflammatory markers", Osteoarthritis Cartilage, 12(6), pp.469~475 (2004)
- Butler SH, "limited arthritic model for chronic pain studies in the rat", Pain, 48(1), pp.73~81 (1992)
- Cheng N, "The effects of electric currents on ATP generation, protein synthesis, and membrane transport of rat skin", Clin Orthop Relat Res, 171, pp.264~272 (1982)
- Cravero JD, "Increased expression of the Akt", PKB inhibitor TRB3 in osteoarthritic chondrocytes inhibits insulin-like growth factor 1-mediated cell survival and proteoglycan synthesis, 2009, pp.60(2), 492~500
- Creamer P, "Novel drug treatment strategies for osteoarthritis", J Rheumatol, 20(9), pp.1461~1464 (1993)
- Demir H, "A comparative study of the effects of electrical stimulation and laser treatment on experimental wound healing in rats", J Rehabil Res Dev, 41(2), pp.147~154 (2004)
- Dowdall T, "Comparison of five different rat models of peripheral nerve injury", Pharmacol Biochem Behav, 80(1), pp.93~108 (2005)
- Duarte LR, "The stimulation of bone growth by ultrasound", Arch Orthop Trauma Surg, 101(3), pp.153~159 (1983)
- Gomez-Camarillo MA, "Chondrocyte proliferation in a new culture system", Cell Prolif, 42(2), pp.207~218 (2009)
- Hochberg MC, "Multidisciplinary integrative approach to treating knee pain in patients with osteoarthritis", Ann Intern Med, 139(9), pp.781~783 (2003)
- Kellgren JH, "Radiological assessment of osteo-arthrosis", Ann Rheum Dis, 16, pp.494~502 (1957)
- Lippiello L, "Collagen synthesis in normal and osteoarthritic human cartilage", J Clin Invest, 59(4), pp.593~600 (1977)
- Lorenz H, "Osteoarthritis: Cellular and molecular changes in degenerating cartilage", Prog Histochem Cytochem, 40(3), pp.135~163 (2006)
- Min SS, "A novel method for convenient assessment of arthritic pain in voluntarily walking rats", Neurosci Lett, 308(2), pp.95~98 (2001)
- Mistry D, "Chondrocyte death during murine osteoarthritis", Osteoarthritis Cartilage, 12(2), pp.131~141 (2004)
- Owoeye I, "Low-intensity pulsed galvanic current and the healing of tenotomized rat achilles tendons: Preliminary report using load-to-breaking measurements", Arch Phys Med Rehabil, 68(7), pp.415~418 (1987)
- Roo CS, "A rapid method for evaluation of analgesic and anti-inflammatory activity in rats", Indian J Exp Biol, 29(2), pp.120~122 (1991)
- Schmidt MB, "A review of the effects of insulin-like growth factor and platelet derived growth factor on in vivo cartilage healing and repair", Osteoarthritis Cartilage, 14(5), pp.403~412 (2006)
- Schött E, "Weight bearing as an objective measure of arthritic pain in the rat", J Pharmacol Toxicol Methods, 31(2), pp.79~83 (1994)
- Vanwanseele B, "The effects of immobilization on the characteristics of articular cartilage: Current concepts and future directions", Osteoarthritis Cartilage, 10(5), pp.408~419 (2002)
- Yin W, "Oxidative stress inhibits insulin-like growth factor-I induction of chondrocyte proteoglycan synthesis through differential regulation of phosphatidylinositol 3-Kinase-Akt and MEK-ERK MAPK signaling pathways", J Biol Chem, 284(46), pp.31972~31981 (2009)