RAW 264.7 대식세포에서 Gelidium amansii의 항염증 효과
Anti-inflammatory Effects of Gelidium amansii in RAW 264.7 Macrophages
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Abstract
In order to verify the anti-inflammatory effects of Gelidium amansii, RAW264.7 macrophages were incubated with the extract of 70% ethanol solution (Ex), and activated with the endotoxin lipopolysaccharide (LPS). Ex inhibited the expression of the pro-inflammatory enzymes, including inducible nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2), and the production of iNOS-mediated NO and COX-2-mediated prostglandin E2 (PGE2) production in a dose-dependent manner. Ex also reduced the release of the pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-6 in LPS-activated macrophages. The observed anti-inflammatory effects of Ex was associated with inactivation of the nuclear factor κB (NF-κB) that mediates the induction of iNOS, COX-2, TNF-α, IL-1β, and IL-6. Further studies showed that Ex inactivated NF-κB through inhibition of phosphorylation of the inhibitory κB (IκB). Taken together, these results suggest that Gelidium amansii exerts anti-inflammatory effects by inhibiting the expression of pro-inflammatory enzymes and the secretion of pro-inflammatory cytokines via inactivation of NF-κB and/or IκB.
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