RAW 264.7 세포에서의 미역(Undaria pinnatifida)뿌리 에탄올 추출물의 항염증활성
Anti-inflammatory Activity of Ethanol Extract of Undaria pinnatifida Root in RAW 264.7 Cells
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The anti-inflammatory effects of the ethanol extract ofUndaria pinnatifida root (UPREE) were investigated using the lipopolysaccharide (LPS)-induced inflammatory response in RAW 264.7 cells by measuring the production of nitric oxide (NO), interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β, and cell proliferation. We found that NO levels were reduced by 34% at 100 μg/mL. Moreover, the production of IL-6 and TNF-α was suppressed by the UPREE treatment. In particular, the IL-6 production was inhibited by more than 30% at 100 μg/mL UPREE. The proliferation of RAW 264.7 cells was measured by MTT assay, and we found no cytotoxicity in those cells treated with UPREE compared to the control. Our results suggest that UPREE shows promise as a therapeutic anti-inflammatory treatment.
Abstract
The anti-inflammatory effects of the ethanol extract ofUndaria pinnatifida root (UPREE) were investigated using the lipopolysaccharide (LPS)-induced inflammatory response in RAW 264.7 cells by measuring the production of nitric oxide (NO), interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β, and cell proliferation. We found that NO levels were reduced by 34% at 100 μg/mL. Moreover, the production of IL-6 and TNF-α was suppressed by the UPREE treatment. In particular, the IL-6 production was inhibited by more than 30% at 100 μg/mL UPREE. The proliferation of RAW 264.7 cells was measured by MTT assay, and we found no cytotoxicity in those cells treated with UPREE compared to the control. Our results suggest that UPREE shows promise as a therapeutic anti-inflammatory treatment.
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