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해양 홍조류 에탄올 추출물의 RAW 264.7 세포와 HEKn 세포에서 항염증 효과

Anti-inflammatory Effects of Ethanolic Extracts from Marine Red Algae on RAW 264.7 and HEKn Cells

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The current study investigated the anti-inflammatory effects of ethanol extracts from marine red algae, namely Gracilaria verrucosa, Gracilaria vermiculophylla, Chondracanthus tenellus, and Gloiopeltis furcata in RAW 264.7 cells and HEKn cells. RT-PCR and Western blot analysis were used to confirm the mRNA expression and protein expression patterns of the major factors. Both the cells did not show cytotoxicity to the four red algae extracts. In the LPS-induced inflammatory cell model, iNOS production decreased with increasing concentration of the extracts. At 100 µg/mL concentrations of the extracts, G. verrucose showed the highest inhibitory activity (63.6%). COX-2 also showed an inhibitory effect of 85% or more in the four extracts. TNF-a and lL-1B showed the strongest inhibitory activities in the G. vermiculophylla (73.9%) and G. furcate (94.7%) extracts. In HEKn cells, the COX-2 production decreased significantly with increasing concentration. At 100 μg/mL extract concentration of iNOS and MMP-2, the most potent inhibitory effect was shown by the G. furcate (73.9%), and in both TNF-α and IL-1β, the G. verrucosa extract showed the strongest inhibitory effects at 47.8% and 41.7%, respectively. The ethanol extracts of the four domestic marine red algae are not cytotoxic, and contain effective substances with high anti-inflammatory properties, making them a functional natural material for cosmetics and pharmaceuticals. The value of their use is expected to be very high.

Abstract

The current study investigated the anti-inflammatory effects of ethanol extracts from marine red algae, namely Gracilaria verrucosa, Gracilaria vermiculophylla, Chondracanthus tenellus, and Gloiopeltis furcata in RAW 264.7 cells and HEKn cells. RT-PCR and Western blot analysis were used to confirm the mRNA expression and protein expression patterns of the major factors. Both the cells did not show cytotoxicity to the four red algae extracts. In the LPS-induced inflammatory cell model, iNOS production decreased with increasing concentration of the extracts. At 100 µg/mL concentrations of the extracts, G. verrucose showed the highest inhibitory activity (63.6%). COX-2 also showed an inhibitory effect of 85% or more in the four extracts. TNF-a and lL-1B showed the strongest inhibitory activities in the G. vermiculophylla (73.9%) and G. furcate (94.7%) extracts. In HEKn cells, the COX-2 production decreased significantly with increasing concentration. At 100 μg/mL extract concentration of iNOS and MMP-2, the most potent inhibitory effect was shown by the G. furcate (73.9%), and in both TNF-α and IL-1β, the G. verrucosa extract showed the strongest inhibitory effects at 47.8% and 41.7%, respectively. The ethanol extracts of the four domestic marine red algae are not cytotoxic, and contain effective substances with high anti-inflammatory properties, making them a functional natural material for cosmetics and pharmaceuticals. The value of their use is expected to be very high.

참고문헌 (44)

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