본문 바로가기
KERD
논문KCI 등재

漆皮 부탄올 분획물이 LPS로 유도된 RAW 264.7 대식세포에 미치는 영향

Effects of Butanol extract from Rhois Vernicifluae Cortex (RVC) in lipopolysaccharides-induced macrophage RAW 264.7 cells

김경준|심성용|송생엽
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2007.04|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제20권 제1호|pp. 1-15

2

조회

0

다운로드

0

인용

0

참고문헌

주제분류한의학

초록

Abstract

Objectives : RVC has long been used for a useful natural agent ameliorating inflammation related symptoms in the folk medicine recipe. This study was performed to investigate effects of RVC on the inflammation and oxidation in RAW 264.7 cells. Methods : The RVC was extracted with 80% ethanol and sequentially partitioned with solvents in order to increase polarity. With the various fractions, we determined the activities on the inflammation and oxidation in RAW 264.7 cells. Results : 1. Among the various solvent extracts of RVC, the butanol fraction showed the most powerful inhibitory ability against nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW 264.7 cells without affecting cell viability. 2. Butanol fraction showed a oxidation inhibition effect by decreasing the DPPH and OH radicals. 3. Butanol fraction exhibited the inhibitory avilities against iNOS and COX-2. 4. Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting analysis revealed that the BuOH fraction provided a primary inhibitor of the iNOS protein and mRNA expression in LPS-induced RAW 264.7 cells. Among the up-regulater molecules of iNOS and COX-2, the BuOh fraction of RVC was shown the inhibitory activity of phoshporylation of c-Jun N-terminal kinase (JNK) 1/2 and threonine protein kinase (AKT), the one of the MAPKs pathway. Conclusion : Thus, the present study suggests that the response of a component of the BuOH fraction to NO generation via iNOS expression provide a important clue to elucidate anti-inflammatory and anti-oxidation mechanism of RVC.

참고문헌 (34)

  1. "大韓藥典外韓藥(生藥)規格集", pp.378~ (2002), 서울: 食品醫藥品安定廳
  2. "옻나무 칠액성분중 Urushiol의 암세포 증식억제 효과" (1998)
  3. "옻나무 추출물이 식이에 의한 비만유도 C57BL", 6 mouse에 미치는 영향, 2003, pp.34(4), ~
  4. "乾漆이 인간 기관지 상피세포 Cytokine 분비와 호산구 Chemotaxis에 미치는 영향" (2005)
  5. "한약재로부터 선발된 옻나무수피 추출물로부터 항산화 활성물질의 분리" (1999)
  6. "옻나무 에탄올 추출물로부터 항산화 활성 물질의 구조동정" (1999)
  7. "Analysis of total phenols and other oxidation substrates and antioxidant by means of Folin-Ciocalteu reagent", 299, pp.152~178 (1999)
  8. "Use of a free radical method to evaluate antioxidant activity", 28, pp.25~30 (1995)
  9. "Hydroxyl radical scavenging effects of species and scavengers from brown mustard (Brassica nigra)", 61, pp.118~123 (1997)
  10. "Maekawa K. Characterization of 2' 7'-dichlorofluorescin fluorescence in dissociated mammalian brain neurons estimation on intracellular content of hydrogen peroxide. Brain Res. 1994", pp.7~
  11. "新免疫學入門", pp.67~ (1997), 서울:地球文化社
  12. "IL-6 mRNA Expression in Mouse Peritoneal Macrophages and NIH3T3 Fibroblasts in Response to Candidaalbicans", 10, pp.15~15 (2000)
  13. "Osawa T. Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-arginine- dependent mechanism acts as synergistically as the major cytolytic mechanism of activated macrophages. J Immunol. 1990", pp.31~
  14. "Intraislet release of intorleukin-1 inhibits beta cell function by inducing beta cell expression of inducible nitric oxide syntheses", 181, pp.559~568 (1995)
  15. "산화질소(NO) 이로운가? 해로운가?. 산화질소의 화학과 응용", 39, pp.52~63 (1999)
  16. "Transcriptional regulation by MAP kinases. Mol", 42, pp.459~467 (1995)
  17. "A role for p38(MAPK)", HSP27 pathway in somooth muscle cell migration, 1999, pp.274, 24211~24219
  18. "The MAPK signaling cascade", 9, pp.726~735 (1995)
  19. "Roth RA. Hepatic and extrahepatic pathobiology of bacterial lipopolysaccharides. Pharmacol Rev. 1993", pp.411~
  20. "Nitric oxide and intestinal inflammation", 109(2), pp.150~158 (2000)
  21. "Antitumorigenic and cytotoxic properties of an ethanol extract derived from Rhus verniciflua Stokes", 1570, pp.181~191 (2002)
  22. "옻나무 목질부에서 분리된 플라보노이드의 이화학적및 생물학적 특성", 31(3), pp.345~350 (2000)
  23. "Vascular endothelial cells synthesize nitric oxide from L-arginine", 333, pp.664~666 (1998)
  24. "Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages", 69, pp.625~635 (2001)
  25. "Inducible nitric oxide synthase. A little bit og good in all of us", 47, pp.6~9 (2000)
  26. "Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated RAW 264.7 cells. a murine macrophage cell line", 28, pp.217~226 (2000)
  27. "Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two-carboline alkaloids extracted from Melia axedarach", 406, pp.301~309 (2000)
  28. "Anti-tumor promoting potential of selected spice ingredients with antioxidative and anti-inflammatory activities. a short review", 40(8), pp.1091~1097 (2002)
  29. "Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals. down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation", pp.243~481 (2001)
  30. "Cyclooxygenase-2 and inflammation in atherosclerosis", 4(2), pp.116~123 (2004)
  31. "Inhibition of lipopoly saccharide-inducible nitric oxide synthase. TNF- and COX-2 expression by sauchinone effects on I-kBa phosphorylation. C", EBP and AP-1 activation, 2003, pp.139, 11~20
  32. "오미소독음이 마우스 복강 대식세포에서 NO의 분비과정에 비치는 영향", 16(2), pp.921~927 (2002)
  33. "New insights into the role of nuclear factor-kappaB in cell growth regulation", 152(9), pp.387~397 (2001)
  34. "Inhibition of LPS-induced NO production by taurine chloramines in macrophage is mediated through Ras-ERK-NF-kB", 70, pp.1352~1360 (2005)

관련 논문AI추천

LPS로 자극된 Raw 264.7 대식세포에서 한약재복합 추출물의 세포독성 및 항염증 효과김세기, 박근혜, 박찬익한국화장품미용학회 · 한국화장품미용학회지2016 · 제6권 제1호한국산 자생 수목 유래 수피추출물의 종양괴사인자 억제효과조재열한국약용작물학회 · 한국약용작물학회지2007 · 제15권 제4호Effect of Forsythiae Fructus Exract on the Release of Inflammatory Mediatorinduced by Lipopolysaccharide in RAW 264.7 Macrophage김희택, Bok Jong You한의병리학회 · 동의생리병리학회지2007 · 제21권 제3호대식세포주 RAW264.7 세포에서 Curcumin의 Lipopolysaccharide 에 의한 Nitric Oxide 생성 억제 효과이용규한국약용작물학회 · 한국약용작물학회지2007 · 제15권 제6호防己 추출물이 LPS로 유도된 Raw 264.7 cell에서의 TNF-α, IL-1β, IL-6 및 Nitric Oxide production에 미치는 영향권영규, 김대희, 김상찬, 변성희, 신상우, 이종록한의병리학회 · 동의생리병리학회지2006 · 제20권 제4호마우스 Raw 264.7 세포에 대한 4종 대황류 추출물의 항염증 활성 비교김승주, 김호경, 문병철, 윤태숙, 이아영, 전명숙, 최고야, 추병길한국약용작물학회 · 한국약용작물학회지2009 · 제17권 제2호

이 간행물의 다른 논문 (23건)

알레르기성 비염 모델에서 통관산(通關散)이 Total IgE, specific IgE 생산에 미치는 영향이승주, 김윤범pp. 16-26황금 에탄올 추출물이 IGF-Ⅱ로 유도된 HIF-1α와 VEGF 발현 억제에 미치는 영향변학성, 김경준pp. 27-37加味玉液湯이 알레르기 반응에 미치는 영향강경화, 총배빈, 김원일, 홍상훈pp. 38-50槐花 추출물의 投與가 Streptozotocin으로유발된 당뇨쥐에 미치는 영향김경준, 김현지pp. 51-65淸熱消毒飮이 HL-60 세포주의 Apoptosis에 미치는 영향박수연, 김종한, 최정화, 배진석pp. 66-79
원문 보기