바이오리액터 배양기에 의한 감자소괴경 대량 생산
Mass production of potato microtubers by bioreactor culture
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참고문헌
초록
조직배양 감자줄기를 1/3 MS 액체배지가 첨가된 18 L 바이오리액터 배양기에서 배양하였을 때 생중량 증식이가장 양호하였고, 1/4 MS 액체배지, 1/2 MS 액체배지 순으로 감자줄기의 생중량이 증가하였다. 감자 소괴경 형성은 감자줄기를 배양한 바이오리액터에서 배지를 따라내고, 소괴경 형성용 배지로 교환하여 어두운 곳에서 암배양하면 소괴경이 형성되었다. 소괴경은 암배양 1주후부터 줄기 마디의 측아에서 생성되기 시작하여 3주 정도에 대부분이 형성되고 1.5 MS 액체배지와 20℃ 온도 조건이 최적이었다. 이 조건에서는 6주 정도 배양하면 18L 바이오리액터 배양기에서 1,000개 이상의 소괴경을 생산할 수 있었다. 어두운 배양조건에서 소괴경이 거의 성장하면 바이오리액터를 빛이 있는 조건으로 옮겨 1주정도 배양한 후 녹화된 소괴경를 수확하였다.
Abstract
Highest increase of biomass was observed when tissue-cultured potato (Solanum tuberosum L. cv. Chubaek)shoots were cultured in a liquid medium containing 1/3 MS solution in a 18 L bioreactor, as compared to 1/4 and 1/2 MS solution. The medium containing 1/4 MS solution showed higher increase of shoot biomass than one containing 1/2 MS solution. Potato microtubers were formed when the medium was exchanged with the medium for microtuber formation and incubated under dark condition. The microtubers were observed first at some axillary buds one week after incubation under dark condition and then at most of the axillary buds by the end of 3 weeks. The 1.5 MS liquid medium and 20℃ were optimal conditions. By the end of 6 weeks, more 1,000 microtubers were formed in the 18 L bioreactor. Then,greened microtubers were harvested after one week culture under light condition.
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