감자 기내 소괴경 형성에서 MS배지 첨가조건 및 최적 coumarin 농도
MS Medium Supplement and Optimum Coumarin Concentration for in vitro Tuberization of Potato
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Abstract
:The efect of MS medium supplement and coumarin on in vitro microtuber formation of potato was investigated. Invitro tuberization of potato by MS medium suplement difered from cultivars used and a potato cultivar, Superior, showed signif-icantly higher microtuberization. In supplementing 3 ml MS medium with 3% sucrose, the number and fresh weight of microtubersignificantly increased in Superior. The optimum medium-suplementing time for effective microtuberization was after 3 weeksculture of in vitro plants under tuber inducing condition. Coumarin added with concentration of 12.5 to 25 mg/ in the tuber induc-ing medium promoted microtuber formation in the potato cultivars, Superior and Dejima.