바이오리액터 배양기에 의한 석곡 유식물체 대량 증식
Mass production of the seedlings of Dendrobium moniliforme using bioreactor culture
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무균 발아된 석곡 종자로부터 생장한 줄기의 액아절편 을 절단하여 배양한 결과, 새로운 원시구경 (protocorm)이 형성되었으며 형성율은 1.0 mg/L BA가 첨가된 MS 기본 배지에서 43.7%로 나타났으며 배지에 치상한 접종체의 수를 1개, 2개, 3개 이상 군락 접종하였더니 1개, 2개 접 종시보다 3개 이상 군락 접종하였을 때 생존율이 95.2% 로 높게 나타났다. 바이오리액터에서 석곡 액아절편으로부터 식물체 생 장은 저농도 배지인 1/4 MS배지에서 잘 자랐고, 배지 농 도가 약간 높은 1/2 MS배지에서는 생장이 현저하게 늦어 졌다. 1/4 MS배지 조건에서 6주간 배양하면 5배 정도로 유식물체 줄기를 증식시킬 수 있었다.
Abstract
Protocorms were newly formed from the culture of axillary buds, obtained in the seedlings of Dendrobium moniliforme in vitro. Its formation ratio was calculated to 43.7% on MS medium containing 1.0 mg/L BA. To test their survival ratio, we gradually increased the inoculation of transplant populations from single to more than three, and then found that the ratio in three populations went up as high as 95.2% rather than those of one or two. In bioreactor, explant obtained from the axillary bud grew well in lower concentration as 1/4 MS medium, while clearly grew slow in a little bit high concentration as 1/2 MS medium. We found that the explant of axillary bud, obtained from the Dendrobium moniliforme seedlings, would grow five times after culturing in a bioreactor for six weeks in 1/4 MS medium.
참고문헌 (18)
- Akita M, "Stimulation of potato (Solanum tuberosum L.) tuberization by semicontinuous liquid medium surface level control", Plant Cell Rep, 13, pp.184~187 (1994)
- 최은정, "포도 왜성대목 ‘5BB’의 생물반응기 배양에서 접종밀도가 식물체 생장,기공 및 광합성 특성에 미치는 영향", Journal of Plant Biotechnology, 35(2), pp.127~132 (2008), 한국식물생명공학회
- Choi SO, "Effect of basal medium and pretreatments on seed germination of temperate Cymbidium species", J Kor Soc Hort Sci, 32, pp.525~532 (1991)
- Chung JD, "Effect of several additives on medium browning and mericlone growth of temperate Cymbidium species", Kor J Hort Sci & Tech, 16, pp.239~241 (1998)
- Chung MY, "Effect of culture media on asymbiotic seed germination and those seedling growth of Calanthe discolor and Habenaria radiata", Kor J Plant Tissue Cult, 25, pp.189~194 (1998)
- Harvais G, "On the physiology of a fungus symbiotic with orchids", Can J Bot, 53, pp.144~155 (1975)
- Hyun MR, "Studies on distributions and morphological characteristics of Calanthe discolor, C. sieboldii, and C. bicolor native to Cheju province", Kor J Hort Sci & Tech, 17:, pp.497~499 (1999)
- Jee SO, "Effects of growth retardants on the morphogenesis and GA-like substance activity of Bletilla striata in vitro", J Kor Soc Hort Sci, 41, pp.409~414 (2000)
- Kim JY, "Effect of cultural conditions on rhizome growth and organogenesis of Cymbidium lancifolium native to korea in vitro", J Kor Soc Hort Sci, 33, pp.471~476 (1992)
- Kim KH, "Studies on the Aseptic multiplication in vitro by protocorm culture of Aerides japonicum and the plantlettransplantation at native field", Res Rep RDA, 32, pp.42~49 (1990)
- Kim KH, "Seed pod formation in cross- and selfpollinated Cymbidium kanran native to Cheju and the rhizome formation on various media", J Kor Soc Hort Sci, 37, pp.152~157 (1996)
- Kim MS, "Effects of days after pollination and media on asymbiotic germination in cross combination of Cymbidium spp", Kor J Hort Sci & Tech, 16, pp.236~238 (1998)
- 의 종간교배에 의한 1대잡종 종자의 기내무균 발아, "한국자원식물학회지", 2008, 341~345, pp.한국자원식물학회
- Knundson L, "A new nutrient solution for the germination of orchid seeds", Amer Orchid Soc Bull, 15, pp.214~217 (1946)
- Lee SS, "Effect of orchid habitat soil on growth of tissue cultured Cymbidium kanran and C. goeringii, and root infection of orchid mycorrhizal fungus", J Kor Soc Hort Sci, 38, pp.176~182 (1997)
- Lee YR, "Effects of BA on flowering of Cymbidium encifolium Tekkotsusosin", Kor J Hort Sci & Tech, 16, pp.531~532 (1998)
- Lo SF, "Bioguided fractionation and isolation of free radical scavenging components from in vitro propagated chinese medicinal plants Dendrobium tosaense Makino and Dendrobium moniliforme SW", J Agric Food Chem, 52, pp.6916~6919 (2004)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)