감자절간배양에서 마디위치 및 잎의 부착유무에 따른 소괴경형성의 차이
Influences by position of node and existence of leaf on microtuberization in node culture of potato
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초록
Single-node stem pieces ca. 1 cm in length containing a axillary bud were obtained from in vitro plants of potato (Solanum tuberosum L.). The influences by a position of the node and the existence of a leaf at the node were observed in the single-node culture on the 8% sucrose MS medium. The effect of CCC was also investigated for the microtuberization. The apical part node was excellent in the tuberization not to mention shoot length, fresh weight, diameter, the number of node on the in vitro culture of a single-node than the lower part. The differences in the diameter of a tuber formed in the part of the axillary bud on all treatments including the cultivation of the apical part node were not recognized. However, the fresh weight of the tuber showed high value in the tuber formed at the axillary bud of shoot apex part. At 20 days after cultivation, tuberization was promoted in the new stolon that developed from the bud of node with a leaf under SD condition of 8 hours at 20℃. The tuberization from axillary bud of the single-node without leaf was inhibited at high temperature of 28℃ regardless of daylength. Whereas, tuberization at 20℃ and 28℃ was similar without the difference under SD condition but the tuber formation ratio were low. CCC 500 mg/L promoted tuberization and the effect was also showed even under LD condition at 28℃. The inhibiton of tuberization under LD and high temperature condition could be solved by treatment with CCC.
Abstract
Single-node stem pieces ca. 1 cm in length containing a axillary bud were obtained from in vitro plants of potato (Solanum tuberosum L.). The influences by a position of the node and the existence of a leaf at the node were observed in the single-node culture on the 8% sucrose MS medium. The effect of CCC was also investigated for the microtuberization. The apical part node was excellent in the tuberization not to mention shoot length, fresh weight, diameter, the number of node on the in vitro culture of a single-node than the lower part. The differences in the diameter of a tuber formed in the part of the axillary bud on all treatments including the cultivation of the apical part node were not recognized. However, the fresh weight of the tuber showed high value in the tuber formed at the axillary bud of shoot apex part. At 20 days after cultivation, tuberization was promoted in the new stolon that developed from the bud of node with a leaf under SD condition of 8 hours at 20℃. The tuberization from axillary bud of the single-node without leaf was inhibited at high temperature of 28℃ regardless of daylength. Whereas, tuberization at 20℃ and 28℃ was similar without the difference under SD condition but the tuber formation ratio were low. CCC 500 mg/L promoted tuberization and the effect was also showed even under LD condition at 28℃. The inhibiton of tuberization under LD and high temperature condition could be solved by treatment with CCC.
참고문헌 (20)
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