액체배지를 이용한 조직배양 감자줄기의 대량증식
Mass production of potato shoots by liquid culture
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참고문헌
초록
MS배지와 PM (Potato medium)배지 (MS배지에서 NH4NO3 와 KNO3의 양은 1/2, CaCl2는 2배 첨가하고 나머지는 동일)에서 대서, 자심, 추백, 하령 4 품종 감자줄기의 기내 대량증식을 위해 실험하 였다. 고체배지에서 배양초기 (1∼2주)에 대서와 자심은 MS배지 에서 줄기의 신장이 가장 우수하였고, 하령과 추백은 PM배지가 효 과적이었지만 배양후기인 4주째에는 두 배지 사이에 차이가 없었 다. 액체 정치배양한 배지에서는 고체배지와 달리 배양초기에는 생장이 늦었고 배양 1주후부터 감자 (4cm 이상) 줄기 발생률이 급 격히 증가하기 시작하였고, 4주까지 꾸준히 생장을 지속하였다. 바 이오리액터에서 줄기의 생장은 품종에 따라 차이를 보였다. 특히 추백과 자심은 액아 발생뿐만 아니라 줄기신장이 1/4 PM배지가 우 수하였고, 대서는 1/2 PM배지에서 액아발생이 왕성하였다. 한편, 하령은 다른 품종에 비해 생장이 늦었다.
Abstract
A study was conducted to investigate in vitro micropropagation of four potato cultivars of Daese, Jasim, Chubaek and Haryeng in MS medium and PM medium (a medium containing half concentration of NH4NO3 and KNO3, two fold concentration of CaCl2, as compared to MS medium). During 1∼2 weeks of culture, Daese and Jasim showed better shoot elongation on the solid MS medium than Chubaek and Haryeng whereas Chubaek and Haryeng did better shoot elongation on the solid PM medium. But no difference was observed after 4 weeks of culture. As compared to shoot elongation on the solid medium, it was delayed at early stage of culture in the liquid medium without shaking. Shoot formation ratio of potato (above 4 cm of shoot length) began to increase significantly after 1 week of culture and kept on increasing until 4 weeks. The four cultivars showed the different patterns of shoot growth in bioreactor. The PM medium with a quarter salt strength was effective for the regeneration of axillary buds as well as shoot elongation of Jasim and Chubaek. Daese showed vigorous regeneration of axillary buds in the PM medium with a half salt strength. On the other hand, Haryeng showed slower growth than the other three cultivars.
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