Membrane Filter와 Sucrose 농도가 도라지 (Platycodon grandiflorum A. DC.) 기내 배종묘의 생장에 미치는 영향
Effects of Membrane Filter and Sucrose Concentrations on the Growth of Balloon Flower (Platycodon grandiflorum A. DC.) Plantlets In Vitro
2
조회
0
다운로드
1
인용
0
참고문헌
초록
기내 배양환경을 조절하여 건전한 유식물체를 획득하기 위해 도라지를 무균발아시켜 shoot 부분을 NAA 0.1 mg/L가 첨가된 MS 기본배지에 치상한 후 membrane filter 부착유무와 sucrose 농도를 달리 처리하여 생장반응을 조사하였다. Membrane filter 미부착구에서는 배양용기 내의 CO2와 C2H4 농도가 membrane filter 부착구에 비해 높았으며 membrane filter가 부착되지 않았을 때 CO2 농도는 배양기간이 경과될수록 증가하였으나 C2H4 농도는 감소하는 경향이었다. 식물체 생장은 membrane filter 부착구가 미부착구에 비해 양호하였고, 미부착구에서는 sucrose 농도가 증가 할수록 생육이 양호하게 나타났다. 생체중과 건물중은 membrane filter를 부착하지 않은 sucrose 4.5% 처리구에 비해 membrane filter를 부착한 sucrose 무첨가구에서 비슷하거나 높았다. 엽록소 함량은 membrane filter 부착구에서 미부착구에 비해 높았으며 당 함량도 비슷한 경향이었는데 두 처리간 차이는 더 컸다. 기공은 membrane filter 부착구에서 미부착구에 비해 닫혀 있었으며 엽육조직은 membrane filter를 부착하거나 sucrose 농도가 높을수록 잘 발달하였다. 또한 상토에 이식한 후 13일째 생존율은 sucrose 처리농도에 관계없이 membrane filter 미부착구는 0%인데 비해 부착구는 100%로 나타났다.
Abstract
The shoots of balloon flower (Platycodon grandiflorum A. DC.) in vitro germinated from seeds were cultured on MS basal medium containing 0.1 mg/L NAA under the various sucrose concentrations and with/without membrane filter (MF) on the lid of vessel. The growth responses were checked to obtain healthy plantlets. The CO2 and C2H4 concentration in vessel without MF were higher than those with MF. The CO2 concentration without MF was increased as days in culture went by whereas the C2H4 concentration was decreased. The plant growth with MF and high sucrose concentration was good. Fresh and dry weight of plantlets cultured in sucrose 4.5% with MF were higher than those in no sucrose without MF. Also the content of chlorophyll of plantlets cultured with MF was high and the content of sugar was shown a similar results and a remarkable difference between MF treatments, especially. Stomata cultured with MF was closer than that without MF and mesophyll of leaf were more developed with MF or in high sucrose concentration. When the plantlets were transplanted in the pot at 25±2℃, 75% relative humidity and low PPFD (photosynthetic photon flux density), the percentage of survival after 13 days without MF was 0% but it was 100% with MF regardless of sucrose concentrations.
참고문헌 (28)
- "Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris", ar(n di), pp.1~15 (1959)
- Broome O, "In Vitro propagation from shoot tips of blackberry", 13, pp.151~153 (1978)
- Chung JD, "Recent Plant Biotechnology" (1995)
- Acta Hort 289, "Acclimatization techniques of plants from in vitro", pp.291~300 (1991)
- Earle ED, "Carnation propagation from shoot tips cultured in liquid medium", 10, pp.608~610 (1975)
- Fujiwara K,, "Physical microenvironment and its effects. In : Aitken-Christie J, Kozai T, Lila Smith M (eds),", pp.319~369 (1995)
- Goo DH, "Growth characteristics of lily by the treatment of aeration in vitro", 26, pp.241~244 (1999)
- Grout BWW, "Transplanting of cauliflower plants regenerated from meristem culture Water loss and water transfer related to changes in leaf wax and to xylem regeneration", pp.1~7 (1977)
- Neth J Plant Path 89, "Some factors affecting glassiness in carnations meristem tip culture", pp.47~53 (1983), Hakkaart FA
- Han BH, "Effect of different sealing materials on CO2 and ethylene concentration in culture vessel and growth and vitrification on Gypsophila paniculata Bristol Fairy'", 37, pp.118~122 (1996)
- Han BH, "Structural characteristics of vitrified and glaucous plantlets in Gypsophila paniculata L", pp.177~189 (1992)
- Hull HM, "Environmental influences on cuticle development and resultant foliar penetration", 41, pp.421~451 (1975)
- Kim KW, "Physiological and morphological characteristics of the glaucous and vitreous carnation plantlets obtaines in vitro", 29, pp.216~223 (1988)
- Cell Cult Somatic Cell Genet Plants 8, "Controlled environments in conventional and automated micropropagation", pp.213~230 (1991)
- Fujiwara K, "Collected papers on environmental control in micropropagation Faculty of Horticulture Chiba Univ" (1994)
- J Jap Soc Hort Sci 57, "Effects of CO2 enrichment and sucrose concentration under high photon fluxes on plantlet growth of carnation in tissue culture during the preparation stage", pp.279~288 (1988)
- Kozai T, "Effects of the number of air changes per hour of the closed vessel and the photosynthetic photon flux on the carbon dioxide concentration inside the vessel and the growth of strawberry in vitro", 26, pp.21~29 (1988)
- Kwon SI, "Effects of growth regulators and culture environment on ex vitro rooting and acclimatization of apple rootstock in vitro propagated", 31, pp.133~138 (2004)
- Lee EJ, "Growth of Limonium Misty Blue' as affected by culture environment in vitro and level of shading during ex vitro acclimatization", 40, pp.623~626 (1999)
- Lee HS, "Structural characteristics of leaves and carbohydrate content of propagules grown at different culture conditions in Gerbera hybrida Beauty'", 28, pp.117~121 (2001)
- "Absorption of light by chlorophyll solutions", pp.315~322 (1941)
- Martin JT, "The cuticles of plants" (1970)
- McCready RM, "Determination of starch and amylose in vegetables", 22, pp.1156~1158 (1950)
- Morris DL, "Quantitative determination of carbohydrates with drywood's anthrone reagent", pp.254~107 255 (1948)
- Paek KY, "Anatomical observation of vitrified and glaucous leaf from Rehmannia glutinosa plant produced in vitro", 24, pp.323~327 (1997)
- Park KW, "Hydroponics in Horticulture" (1998), Academy Publisher
- Sharkel KA, "Stomatal function and cuticular conductance in whole tissue cultured apple plants", 115, pp.468~472 (1990)
- Sutter EG, "Epicuticular wax formation on carnation plantlets regenerated from shoot tip culture", 104, pp.493~496 (1979)