기내배양 사과 대목의 기외 삽목 시 발근과 순화에 미치는 배양조건 및 생장조절물질의 효과
Effects of Growth Regulators and Culture Environment on ex vitro Rooting and Acclimatization of Apple Rootstock in vitro Propagated
1
조회
0
다운로드
1
인용
0
참고문헌
초록
본 연구는 사과 왜성대목 M.9와 M.26 조직 배양묘를 통기성 마개와 비통기성 마개의 MS배지에서 생장 특성을 비교하기 위해 시험되었다. 또한 배양묘의 생육 변화와 이 배양묘를 발근촉진제 침지 처리 후 기외 삽목을 하여 발근 및 순화를 동시에 시키기 위해 실시하였다. 배양병내의 CO2와 에틸렌의 농도는 통기성 마개 배양병이 비통기성마개 배양병 보다 낮았다. 통기성 마개 배양병내의 온․습도 변화는 광조건에 따라 일정하게 반복되었다. 통기성 마개 배양묘의 기공은 외기에서 즉시 관찰 시 닫혀 있었으나 비통기성마개 배양묘의 기공은 외기에서 20분 후 관찰 시에도 열려 있었다. 통기성 마개 배양묘의 엽록체 지수와 잎 면적은 모두 비통기성 마개의 것 보다 높았다. 기외 삽목시 발근율은 M.9와 M.26 모두 300 mg/L IBA + 3% sucrose 침지 처리구에서 각각 86.5%와 85.4%로 가장 높았으며 순화율은 각각 71.7%와 70.0%로 가장 높았다. 통기성 마개 배양묘의 순화율은 비통기성마개 배양묘 보다 모든 침지 처리구에서 순화율이 비슷하거나 높았다.
Abstract
Growth of M.9 (Malus domestica Bork. cv) and M.26 (Malus domestica Bork. cv) of dwarf apple rootstock, cultured on MS agar medium in a vessel with ventilating stopper (VS) and then in vivo rooting and acclimatization under combined-treatment by some materials with IBA, were investigated. Concentration of CO2 and ethylene in the vessel with VS was lower then in the vessel with non-VS. Change of temperature and humidity in the vessel with VS was repeated by light condition. Stomatal pores of tissue in the vessel with VS were immediately closed after plantlets were exposed to room humidity but those in the vessel with non-VS were opened after 20 minutes exposure to room humidity. Leaf area and chloroplast index of tissue in the vessel with VS was higher then in the vessel with non-VS. In vivo rooting ratio and acclimatization ratio of M.9 and M.26 was highest in 300 mg/L IBA+3% sucrose dip-treatment among other combined- treatments.
참고문헌 (12)
- Alvarez R, "Relationship between indole-3-acetic acid levels in apple rootstocks cultured in vitro and adventitious root formation in the presence of indole-3-butyric acid", pp.439~443 (1989)
- Carmen DR, "Effects of carbohydrate content on the seasonal rooting of vegetative and reproductive cuttings of Olive", 66, pp.301~309 (1991)
- Desjardins YF, "Effect of CO2 enrichment and high phothsynthetic photon flux on the development of autotrophy and growth of tissue culture strawberry", pp.45~53 (1988)
- Infante R, "The role of light and CO2 in optimizing the conditions for shoot proliferation of Actinidia deliciosa in vitro", 77, pp.191~195 (1989)
- Jeong BR, "Photoautotrophic growth of Dianthus caryophyllus in vitro as affected by photosynthetic photon flux and CO2 condition", 440, pp.611~615 (1996)
- Hayashi M, "Environmental control for acclimation in vitro cultured plantlets Developement of the acclimatizaton unit for accelerating the plantlet growth and the test cultivation", pp.349~358 (1987)
- "Environmental control for production of quality plantlets in vitro at low costs in a large scale Advances in Developmental Biology and Biotechnology of Higher Plants Proceedings of the first Asia- Pacific Conference on Plant Cell and Tissue Culture pp 71-100" (1993)
- Kwon SI,, "Effect of various mixed-treatment by some growth regulators, sugars and inorganic matters with IBA on rooting in hardwood cuttings of M.26 apple rootstock", 40, pp.447~450 (1999)
- Lee CH, "Effects of growth regulators and medium composition on the growth of each stage in shoot tip culture of apple rootstock M", pp.385~392 (1990)
- Lee EJ, "Study on the autotrophic culture of Limonium spp. cv." (1998), Kyung Sang University
- Seo HS, "Study on cutting propagation of apple", pp.245~247 (1983)
- Yae BW, "Factors affecting shoot proliferation and root initiation of apple Fuji' in vitro", 27, pp.353~358 (1986)