배양용기 내 환기와 광도에 따른 도라지(Platycodon grandiflorum A. DC.) 기내 배양묘의 생장반응
The Growth Response of Balloon Flower(Platycodon grandiflorum A. DC.) Plantlets In Vitro as Affected by Air Exchanges and Light Intensity
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기내 배양환경을 조절하여 건전한 유식물체를 획득하기 위해 도라지를 무균발아시켜 shoot 부분을 NAA 0.1 mg/L가 첨가된 MS 기본배지에 치상한 후 배양용기의 뚜껑위에 membrane filter를 부착하여 환기횟수를 달리하고 광도를 33, 66, 99 μ㏖ m-2s-1로 처리하여 생장반응을 조사하였다. Membrane filter 부착구의 환기횟수는 4.9 h-1이었으며 미부착구는 0.1 h-1이었다. 환기횟수 4.9 h-1 처리구에서 0.1 h-1 처리구에 비해 전반적으로 생장이 양호하였으며 0.1 h-1에서는 광도가 증가할수록 생장이 저조한 반면 4.9 h-1 처리구에서는 광도의 증가에 따라 생장이 좋은 경향이었다. 동일한 광도에서 환기횟수 4.9 h-1 처리구는 0.1 h-1 처리구에 비해 생체중은 1.9배, 당 함량은 1.4배 이상 높았으며 환기횟수 4.9 h-1 처리구는 광도의 증가에 따라 생체중과 당 함량이 높아졌으나 0.1 h-1 처리구는 광도에 의한 효과가 인정되지 않았다. 함수율은 환기횟수 4.9 h-1 처리구에서 0.1 h-1 처리구에 비해 4.2～5.8%가 낮았으나 광도 처리에 의한 차이는 없었다. 동일한 광도에서 총 엽록소 함량은 환기횟수 4.9 h-1 처리구에서 0.1 h-1 처리구에 비해 0.27∼0.79 mg/g F.W.가 높았으며 환기횟수 0.1 h-1 처리구는 광도의 증가에 따른 효과가 나타나지 않았으나 환기횟수 0.1 h-1 처리구는 높은 광도에서 감소하였다. 환기횟수 4.9 h-1는 0.1 h-1에 비해 잎의 공변세포가 크고 주변 부세포가 잘 발달되어 있었다. 특히 PPF 99 μ㏖ m-2s-1에서 환기횟수 0.1 h-1는 부세포의 발달이 미흡하고 기공이 많이 열려 있는 상태인 반면 환기횟수 4.9 h-1는 부세포가 잘 발달된 잎을 지니고 있었다.
Abstract
Shoots of balloon flower (Platycodon grandiflorum A. DC.) derived from in vitro germinated seeds were cultured on MS medium containing 0.1 mg/L NAA under various photosynthetic photon flux (PPF) 33, 66, and 99 μ㏖ m-2s-1 with or without membrane filter. Number of air exchanges per hour (NAEH) of the culture vessel with membrane filter on the lid was 4.9 h-1 and that without membrane filter was 0.1 h-1. Plantlets grown in 4.9 h-1 NAEH showed greater growth than in 0.1 h-1 NAEH. According to increase of PPF, plantlets growth decreased in 0.1 h-1 NAEH while it increased in 4.9 h-1 NAEH. At the same PPF, fresh weight and sugar content in plantlets in 4.9 h-1 NAEH were above 1.9, 2.0 times higher than those in 0.1 h-1 NAEH, respectively. Also they were enhanced in 4.9 h-1 NAEH by increase of PPF whereas no significance in 0.1 h-1 NAEH. The percentage of water content of plantlets in 4.9 h-1 NAEH was 4.2～5.5% lower than those in 0.1 h-1 and no difference in PPF. The content of total chlorophyll in plantlets in 4.9 h-1 NAEH was higher 0.27～0.79 mg/g F.W. than that in 0.1 h-1 NAEH. By increase of PPF, it was decreased in 0.1 h-1 NAEH while had no significant difference in 4.9 h-1 NAEH. Guard and subsidiary cells of leaves in 4.9 h-1 NAEH were more developed than in 0.1 h-1 NAEH. Especially, in 99 μ㏖ m-2s-1, leaves in 0.1 h-1 NAEH had undeveloped subsidiary cells and wide open stomata whereas those in 4.9 h-1 NAEH had well-developed subsidiary cells.
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