‘자랑’ 포도 생장점 배양으로부터 무병주 식물 대량번식에 미치는 배지 구성물질과 생장 조절제의 영향
Effect of Medium Composition and Growth Regulators on Mass Propagation of Virus‐Free Plant from the Meristem Cultures of ‘Jarang’ Grape
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본 연구는 바이러스 무병묘의 대량 증식을 위한 기초 자료를 얻고자 포도 ‘자랑’ 품종의 신초와 뿌리의 형성 및 생장에 미치는 배지조성물질과 생장조절제의 영향을 구명하고자 수행되었다. 배지의 무기염 농도가 낮을수록 신초의형성은 많아지는 경향으로, 특히 1/2MS배지에서 신초의 형성 및 생장이 가장 양호하였다. 부정근의 형성은 무기염의농도가 높아질수록 양호하여 2MS배지에서 부정근의 수가2.1개로 가장 많았다. Sucrose의 경우, 신초의 형성은 1%,부정근의 형성은 3%, 생장은 1% 농도구에서 가장 좋았다. 활성탄의 0.05% 첨가에 의해 신초의 생장은 양호했으며, 특히, 부정근의 형성 및 생장에 효과적이었다. ‘자랑’의 대량증식을 위한 배지의 pH는 6.8이 가장 적합하여 신초의 마디수와 길이가 각각 3.9개와 1.3 cm로 가장 좋았다. 기내 대량증식에 미치는 생장조절제의 영향으로 BA 1.0 mg/L에서신초의 생장이 가장 왕성하였으며, NAA 1.0 mg/L에서 절편체당 16.9개의 신초가 형성되어 양호한 결과를 얻었다.
Abstract
This study was performed to clarify the effect of medium compositions and plant growth regulators on the shoot,root formation and growth of ‘Jarang’ grape for mass propagation of virus‐free plant. The formation and growth of shootwere considerably favorable in half‐concentration of MS medium. However, the formation of adventitious root per explants(avg. 2.1) was effective in higher concentration (two times) of MS medium. For sucrose concentration, 1% for the shootformation, 3% for the adventitious root formation and 1% for the growth were observed as yield significant results. With theaddition of 0.05% of activated carbon, the shoot growth was improved, and it was effective for the adventitious rootformation and growth as well. A pH of 6.8 in the medium was the most suitable for mass propagation; the results showedsignificant enhancement in the number of nodes and the length of the shoot, 3.9 and 1.3 cm, respectively. The shoot growthwas the most vigorous in BA 1.0 mg/L due to the impact of the growth regulator on the mass propagation in it. Consequently, 16.9 shoots per explant were formed in NAA 1.0 mg/L so good results were obtained.
참고문헌 (32)
- Anderson, W. C, "Production of rhododendrons by tissue culture. I. Development of a culture medium for multiplication of shoots", Proc. Int. Plant Prop. Soc, 25, pp.129~135 (1975)
- Chee, R., "In vitro propagation of Vitis : Application of genotypes", Vitis, 22, pp.363~374 (1983)
- Chee, R., "The effects of growth substances and photoperiod on the development of shoot apices of Vitis cultured in vitro", Sci. Horticult., 16, pp.17~27 (1987)
- Choi, I. M., "Effects of medium compositions and on shoot multiplication and rooting in shoot tip cultures of ‘Kyoho’ and ‘Campbell Early’ grapes", Kor. J. Plant Tiss. Cult, 19, pp.59~66 (1992)
- Choi, S. Y., "Studies on the meristem culture in grapevine(Vitis)", J. Kor. Soc. Hort. Abstracts, 6, pp.164~165 (1988)
- Engelbrecht, D. J., "Absence of grapevine virus a correlated with elimination of leafroll disease", ICVG, pp.159~163 (1989)
- Fukui, H., "Varietal differences in shoot tip culture of Japanese persimmon (Diospyros Kaki Thunb.)", J. Jap. Soc. Hort. Sci, 59, pp.51~57 (1990)
- Gautheret, R. J, "Investigations on the root formation in the tissue of Helianthus tuberosus cultured in vitro", Amer. J. Bot, 56, pp.702~717 (1969)
- George, E. F., "Plant propagation by tissue culture", pp.284~307 (1984), British Library
- Goussard, P. G, "Effects of cytokinins on elongation, proliferation and total mass of shoots derived from shoot apices of grapevine cultured in vitro", Vitis, 20, pp.228~234 (1981)
- Hwang, J. H., "Effect of plant growth regulators on in vitro growth of differentially chilled grape shoots", J. Kor. Soc. Hort. Sci, 31, pp.142~149 (1990)
- Jarret, R. L., "Chemical and environmental growth regulation of sweet potato (Ipomoea batatas(L.) Lam.) in vitr", Plant Cell Tiss. Org. Cult, 25, pp.153~159 (1991)
- Jona, R., "Callus and axillary bud culture of Vitis vinifera, ‘Sylvaner Riesling’", Hort. Sci., 9, pp.55~60 (1978)
- 김승희, "포도의 기내생장에 미치는 시토키닌의 영향", Journal of Plant Biotechnology, 29(2), pp.123~127 (2002), 한국식물생명공학회
- Krul, W. R., "Formation of adventitious embryos in callus cultures of ‘Seyval’ a French grape", J. Amer. Soc. Hort. Sci, 102, pp.360~363 (1997)
- Kwon, Y. J., "Effects of medium composition and culture condition on plant regeneration via organogenesis of ‘Kyoho’ Grape", J. Kor. Soc. Hort. Sci, 41, pp.276~280 (2000)
- Lee, H. J., "Effect of BA and strength of media on in vitro growth of grapes", Res. Rep. Agr. Sci., Chungbuk Nat’l Univ, 7, pp.17~26 (1989)
- Lee, J. S., "Studies on Cymbidium Kanran native to Korea. I. On rhizome culture in vitro", J. Kor. Soc. Hort. Sci, 25, pp.129~135 (1984)
- Li J. R., "Growth and rooting of grape shoot apices in vitro", Hort. Sci., 19, pp.64~66 (1894)
- Miller, G. A., "In vitro propagation of ‘Nemagard’ peach rootstock", Hort. Sci., 17, pp.194~ (1982)
- Murashige, T., "A revised medium for rapid growth and bioassay with tobacco tissue culture", Physiol. Plant, 15, pp.473~479 (1962)
- Park, H. B., "Somatic embryogenesis and plant regeneration from immature ovule of Vitis flexuosa Thunberg", Kor. J. Plant Tiss. Cult, 20, pp.109~112 (1993)
- Pool, R. M., "The influence of cytokinins in vitro shoot development of ‘Concord’ grape", J. Amer. Soc. Hort. Sci, 100, pp.200~202 (1975)
- Sadamatsu, M. O, "Virus free stock production by shoot-tip culture of grape", Plant Production, 41, pp.418~422 (1987)
- Schnapp, S. R., "In vitro growth retardation of tomato and carnation microplants", Plant Cell Tiss. Org. Cult, 6, pp.3~8 (1986)
- Singha, Suman, "In vitro propagation of crabapple cultivars", Hort. Sci., 17, pp.191~192 (1982)
- Snir, I., "In vitro propagation of malling merton apple root stocks", Hort. Sci., 15, pp.597~598 (1980)
- Stamp, J. A., "Direct shoot organogenesis and plant regeneration from leaves of grape (Vitis sp.)", Plant Cell Tiss. Org. Cult, 22, pp.127~133 (1990)
- Suh, J. H., "Effect of plant growth regulators on multiple shoot formation and elongation from shoot tip cultures of grape species", Kor. J. Plant Tiss. Cult, 28, pp.25~32 (2001)
- Tang, F. A., "Adventitious bud formation in leaf explants of some grapevine rookstock and scion cultivars", Vitis, 29, pp.151~158 (1990)
- Yang, D. C., "Effects of pH, sucrose and vitamins on the growth and tropane alkaloid production of hairy roots of Datura stramonium var. tatula Torr", Kor. J. Plant Tiss. Cult, 24, pp.143~148 (1997)
- Yu D. H., "The influence of explant origin on tissue browning and shoot production in shoot tip cultures of grapevine", J. Amer. Soc. Hort. Sci, 111, pp.972~975 (1986)