도라지 (Platycodon grandiflorum (Jacq.) A. DC.) 부정아 형성을 통한 식물체 재분화
Plant Regeneration via Adventitious Shoot Formation in Platycodon grandiflorum (Jacq. A. DC.)
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
To investigate optimal conditions for plant regeneration in Platycodon grandiflorum (Jacq. A. DC.).Both leaf and hypocotyl explants were cultured on Murashige& Skoog’s (MS) medium supplemented with combinations of 0.1, 0.5, 1.0, or 2.0 mg/L cytokinins (BA and kinetin) and 1.0 mg/L 2,4-D for 6 weeks, respectively. According to the type of explant, the total shoot organogenesis (56.38%) in leaf explants was higher than in hypocotyls (28.20%). In comparison with kinetin and BA for the plant regeneration, the frequency (70.38%) of leaf explants was higher in combination with kinetin and 2,4-D than of BA with 2,4-D (42.38%), whereas the frequency (35.56%) of hypocotyls explants was higher in BA combination than kinetin combination (20.83%). Thehighest frequency (94.20%) was observed from the cultures of leaf explants on the MS medium supplemented with 1.0 mg/L kinetin and 1.0 mg/L 2,4-D. Upon transfer onto 1 /2 MS basal medium containing 3% sucrose, shoots developed into plantlets with roots, and were well grown in soil in the greenhouse. These results lead us to speculate that the optimization of culture conditions was responsible for the mass propagation from in vitro cultures of Platycodon grandiflorum (Jacq. A. DC.).
Abstract
To investigate optimal conditions for plant regeneration in Platycodon grandiflorum (Jacq. A. DC.).Both leaf and hypocotyl explants were cultured on Murashige& Skoog’s (MS) medium supplemented with combinations of 0.1, 0.5, 1.0, or 2.0 mg/L cytokinins (BA and kinetin) and 1.0 mg/L 2,4-D for 6 weeks, respectively. According to the type of explant, the total shoot organogenesis (56.38%) in leaf explants was higher than in hypocotyls (28.20%). In comparison with kinetin and BA for the plant regeneration, the frequency (70.38%) of leaf explants was higher in combination with kinetin and 2,4-D than of BA with 2,4-D (42.38%), whereas the frequency (35.56%) of hypocotyls explants was higher in BA combination than kinetin combination (20.83%). Thehighest frequency (94.20%) was observed from the cultures of leaf explants on the MS medium supplemented with 1.0 mg/L kinetin and 1.0 mg/L 2,4-D. Upon transfer onto 1 /2 MS basal medium containing 3% sucrose, shoots developed into plantlets with roots, and were well grown in soil in the greenhouse. These results lead us to speculate that the optimization of culture conditions was responsible for the mass propagation from in vitro cultures of Platycodon grandiflorum (Jacq. A. DC.).
참고문헌 (24)
- Barwale UB, "Screening of Glycine max and Glycine soja genotypes for multiple shoot formation at the cotyledonary node", Theor. Appl. Genet, 72, pp.423~428 (1986)
- Cho DY, "Plant regeneration from somatic embryo with structural diversity from leaf explants cultures of Osteicum koreanum Kitagawa", Kor J Plant Tiss Cult, 25, pp.51~56 (1998)
- Cho HJ, "Improved shoot regeneration protocol for hairy roots of the legume Astragalus sinicus", Plant Cell, Tiss Org Cult, 69(3), pp.259~269 (2002)
- 최필선, "대두 재조합순계주에서 고빈도 체세포배발생능 계통 조사", Journal of Plant Biotechnology, 29(2), pp.135~138 (2002), 한국식물생명공학회
- 최소라, "Membrane Filter와 Sucrose 농도가 도라지 (Platycodon grandiflorum A. DC.) 기내 배종묘의 생장에 미치는 영향", Journal of Plant Biotechnology, 31(3), pp.209~217 (2004), 한국식물생명공학회
- Chung JH, "Tissue cultures of Platycodon grandiflour", J Agricult Life Sci, 36, pp.9~18 (2002)
- Eun JS, "Effects of light emitting diodes on growth and morphogenesis of in vitro seedling in Platycodon geandiflorum", Kor J Plant Tiss Cult, 27, pp.71~75 (2000)
- Erisen S, "Callus induction and plant regeneration of endemic Astragalus nezaketae in Turkey", Electron of Biotechnol (2010)[DOI]
- Gill R, "Direct somatic embryogenesis and regeneration of plants from seedling explants of peanut (Arachis hypogaea): promotive role of thidiazuron", Can J Bot, 70, pp.1186~1192 (1992)
- Han C, "Induction of plantlets in the anther culture of Platycodon grandiflorum", Kor J. Plant Tiss Cult, 4, pp.1~3 (1976)
- 한은희, "장백도라지의 대량 증식을 위한 조직배양 및 순화 조건 확립", Journal of Plant Biotechnology, 41(3), pp.134~139 (2014), 한국식물생명공학회
- Hou SW, "High frequency plant regeneration from Astragalus melilotoides hypocotyl and stem explants via somatic embryogenesis and organogenesis", Plant Cell Tiss Org Cult, 79(1), pp.95~100 (2004)
- Kasumi M, "Adventitious buf formation and plant regeneration from ovary explants in balloon flower (Platycodon geandiflorum)", J Ja Soc Hort Sci, 75, pp.284~286 (2006)
- Kim SW, "Somatic embryogenesis and plant regeneration in zygotic embryo cultures of balloon flower", Plant Cell Tiss Org Cult, 58, pp.227~230 (1999)
- Ko JA, "Effect of low temperature pretreatment on pollen dimorphism and embryo formation in anther culture of Platycodon grandiflorum", Kor J Plant Tiss Cult, 26, pp.149~156 (1999)
- Ko JA, "Somatic embryogenesis and plant regeneration from leaf and stem culture of Platycodon grandiflorum", Kor J. Breeding, 25, pp.337~343 (1993)
- Lee BK, "Embryogenesis and plnat regeneration in anther cultures of Platycodon grandiflorum", Kor J Plant Tiss Cult, 20, pp.153~157 (1993)
- 이신우, "국내의 도라지 생산현황과 경남의 전망", 한국국제농업개발학회지, 26(1), pp.68~72 (2014), 한국국제농업개발학회[DOI]
- Malik KA, "Regeneration in Phaseoulus vulgaris seedling by N 6-benzylaminopurine and thidiazuron", Planta, 186, pp.384~389 (1992)
- Murashige T, "A revised medium for rapid growth and bioassay with tobacco tissue culture", Physiol Plant, 15, pp.473~479 (1962)
- 박민선, "자운영(Astragalus sinicus L.) 배축절편으로부터 식물체 재생", Journal of Plant Biotechnology, 42(4), pp.396~400 (2015), 한국식물생명공학회[DOI]
- Soh WY, "Multicotyledonary structure of somatic embryo formed cell cultures of Daucus carota L", Korean J Bot, 39, pp.71~79 (1996)
- Turgut-Kara N, "In vitro plant regeneration from embryogenic cell suspension culture of Astragalus chrysochlorus (Leguminoseae)", Afr J Biotechnol, 7, pp.1250~1255 (2008)
- Wright MS, "Plant regeneration by organogenesis in Glycine max", Plant Cell Rep, 5, pp.150~154 (1986)