장백도라지의 대량 증식을 위한 조직배양 및 순화 조건 확립
Establishment of tissue culture and acclimation of white balloon flower (Platycodon grandiflorum DC. cv. Jangback) for the raising of in vitro propagated seedlings
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초록
The aim of this study was to establish the conditionof regeneration for white balloon flower (Platycodongrandiflorum DC. cv. Jangback) and to manage for theraising of seedling with in vitro regenerated plants. It wasexamined that 0.5 mg/L of NAA and 1.0 mg/L of BA was thebest composition for the callus and shoot induction (up to600%). NAA was better than IBA for the induction of rootand it took 16.9 days for the induction of rooting on the MSsoild media containing 0.5 mg/L of NAA and the final rootingratio was up to 75%. Out of 5 different bed soils purchasedfrom local market, “Tosil” was identified to be the best forthe acclimation and growth of in vitro regenerated balloonflower. In detail, on 8 weeks after planting of in vitroregenerated plants in pots containing “Tosil” bed soils, theplant hight was increased up to 2-fold (12.8 cm), 3.5-fold(27) for the number of leaf and 1.5-fold (4.5 cm) for the leaflength when compared to the other four bed soils, respectively. Our preliminary results indicate that it is possible to preventthe occurrence of blue balloon flower in the massivecultivated area of white balloon flower by providing theseedlings raised from in vitro regenerated plants.
Abstract
The aim of this study was to establish the conditionof regeneration for white balloon flower (Platycodongrandiflorum DC. cv. Jangback) and to manage for theraising of seedling with in vitro regenerated plants. It wasexamined that 0.5 mg/L of NAA and 1.0 mg/L of BA was thebest composition for the callus and shoot induction (up to600%). NAA was better than IBA for the induction of rootand it took 16.9 days for the induction of rooting on the MSsoild media containing 0.5 mg/L of NAA and the final rootingratio was up to 75%. Out of 5 different bed soils purchasedfrom local market, “Tosil” was identified to be the best forthe acclimation and growth of in vitro regenerated balloonflower. In detail, on 8 weeks after planting of in vitroregenerated plants in pots containing “Tosil” bed soils, theplant hight was increased up to 2-fold (12.8 cm), 3.5-fold(27) for the number of leaf and 1.5-fold (4.5 cm) for the leaflength when compared to the other four bed soils, respectively. Our preliminary results indicate that it is possible to preventthe occurrence of blue balloon flower in the massivecultivated area of white balloon flower by providing theseedlings raised from in vitro regenerated plants.
참고문헌 (14)
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