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장기간 지구성 훈련은 생쥐 골격근의 slow twitch myosin heavy chain 발현과 미토콘드리아 생합성을 증가시킨다.

Long-term endurance training increases expression of slow twitch myosin heavy chain and mitochondria biogenesis in mouse skeletal muscle

김기진|서현규|고진호
한국운동생리학회|2016.02|운동과학 (Exercise Science)|제25권 제1호|pp. 43-49|DOI 10.15857/ksep.2016.25.1.43

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키워드

mitochondriamuscle fiber typeSERCA2along-term endurance exercisemyosin heavy chain

초록

PURPOSE: The purpose of this study was to identify spatial distribution of type I myosin heavy chain among different muscle fibers by endurance exercise for 8 week. METHODS: Animal were trained for 8 week on treadmill. To avoid acute exercise effect of training, Tibialis anterior(TA) muscles were dissected 18h after last bout of exercise. To evaluate different type myosin heavy chain and mitochondria expression, we performed multicolor immunofluorescence analysis and western-blotting. RESULTS: Type IIb and IIx in trained skeletal muscle for 8 weeks were decreased 46% and 51% respectively than sedentary muscle. Type IIa and I in trained skeletal muscle for 8 weeks were increased 2.2 and 1.7 fold respectively than sedentary muscle. ATP synthase, cytochrome C, NADH and SERCA2a in trained skeletal muscle for 8 weeks were increased 2.5, 2.2, 2.8 and 2.5 fold respectively than sedentary muscle. TA muscles were increased hybrid fiber type distribution, type I and IIa MHC was increased at all type II fiber in trained muscles than sedentary muscle. CONCLUSIONS: Our result raise the possibility that the muscle fiber type conversion was processed which type I or IIa MHC increased at all type II muscle fiber instead of step by step way(type IIb to IIx, type IIx to IIa) by long term endurance exercise for 8 week.

Abstract

PURPOSE: The purpose of this study was to identify spatial distribution of type I myosin heavy chain among different muscle fibers by endurance exercise for 8 week. METHODS: Animal were trained for 8 week on treadmill. To avoid acute exercise effect of training, Tibialis anterior(TA) muscles were dissected 18h after last bout of exercise. To evaluate different type myosin heavy chain and mitochondria expression, we performed multicolor immunofluorescence analysis and western-blotting. RESULTS: Type IIb and IIx in trained skeletal muscle for 8 weeks were decreased 46% and 51% respectively than sedentary muscle. Type IIa and I in trained skeletal muscle for 8 weeks were increased 2.2 and 1.7 fold respectively than sedentary muscle. ATP synthase, cytochrome C, NADH and SERCA2a in trained skeletal muscle for 8 weeks were increased 2.5, 2.2, 2.8 and 2.5 fold respectively than sedentary muscle. TA muscles were increased hybrid fiber type distribution, type I and IIa MHC was increased at all type II fiber in trained muscles than sedentary muscle. CONCLUSIONS: Our result raise the possibility that the muscle fiber type conversion was processed which type I or IIa MHC increased at all type II muscle fiber instead of step by step way(type IIb to IIx, type IIx to IIa) by long term endurance exercise for 8 week.

참고문헌 (35)

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