저산소 환경의 지구성 운동이 근육 위성세포의 활성 및 증식에 미치는 효과
Treadmill Exercise Increases Muscle Satellite Cell Activation Independent of Oxygen Concentration in Rat
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PURPOSE: This study was performed to investigate the effect of hypoxia in conjugation with endurance exercise training on skeletal muscle satellite cell activity in rats. METHODS: Sprague-Dawley rats (20 weeks, N=21) were randomly divided into Control (Con, n=7), normoxia exercise (NE, n=7), and hypoxia exercise (HE, n=7) groups. Exercise training was performed on a rodent treadmill at a frequency of 5 days/week for 4 weeks, with gradual increases in exercise intensity. The rats were sacrificed 48 hours following the last exercise session. We conducted immunohistochemistry and western-blot for evaluating satellite cell cycle in phenotype and protein levels. RESULTS: Any groups did not increase body weight, muscle weight and cross-sectional area were not increased in any groups after 4 weeks (p>.05). Satellite cell proliferation improved in NE (p=.001) and HE (p<.001) groups. NE (p=.028) and HE (p<.001) groups significantly increased the number of centrally-located myonucei compared to Con. CONCLUSIONS: Treadmill exercise can induce satellite cell proliferation and differentiation regardless of oxygen partial pressure in the air, and increasing muscle metabolism efficiency is expected through muscle remodeling by satellite cell activation.
Abstract
PURPOSE: This study was performed to investigate the effect of hypoxia in conjugation with endurance exercise training on skeletal muscle satellite cell activity in rats. METHODS: Sprague-Dawley rats (20 weeks, N=21) were randomly divided into Control (Con, n=7), normoxia exercise (NE, n=7), and hypoxia exercise (HE, n=7) groups. Exercise training was performed on a rodent treadmill at a frequency of 5 days/week for 4 weeks, with gradual increases in exercise intensity. The rats were sacrificed 48 hours following the last exercise session. We conducted immunohistochemistry and western-blot for evaluating satellite cell cycle in phenotype and protein levels. RESULTS: Any groups did not increase body weight, muscle weight and cross-sectional area were not increased in any groups after 4 weeks (p>.05). Satellite cell proliferation improved in NE (p=.001) and HE (p<.001) groups. NE (p=.028) and HE (p<.001) groups significantly increased the number of centrally-located myonucei compared to Con. CONCLUSIONS: Treadmill exercise can induce satellite cell proliferation and differentiation regardless of oxygen partial pressure in the air, and increasing muscle metabolism efficiency is expected through muscle remodeling by satellite cell activation.
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