장염비브리오가 보유하는 ß-lactamase (VPA0477)의 유전학적 특성
Genetic Characterization of ß-lactamase (VPA0477) in Vibrio parahaemolyticus
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Using 108 strains of Vibrio parahaemolyticus isolated from seawater, we investigated ampicillin-resistance profiles and the genetic characterization of ß-lactamase (VPA0477). All of the strains studied, except one strain, were resistant to ampicillin. However, the strain that was susceptible to ampicillin had the same ß-lactamase gene as the ampicillinresistant strains. We compared ß-lactamase promoter region sequences among five strains, including both ampicillinresistant and -susceptible strains. In the susceptible strain, a nucleotide at position -19 in the methionine initiation codon for ß-lactamase was not present in the ampicillin-resistant strains. The genes in the region containing the gene VPA0477 were present in all of the tested strains, and LA-PCR analysis showed that the distance between VPA0474and VPA0479 in all of the V. parahaemolyticus samples was precisely 5.7 kb. In V. parahaemolyticus ß-lactamase,four important structural features that are conserved in Class A ß-lactamases were present in the deduced amino acid sequences. Taken together, our study demonstrates that V. parahaemolyticus ß-lactamase is included in the Class A ßlactamase group, and some nucleotides within the promoter region are of particular importance for ß-lactamase activity.
Abstract
Using 108 strains of Vibrio parahaemolyticus isolated from seawater, we investigated ampicillin-resistance profiles and the genetic characterization of ß-lactamase (VPA0477). All of the strains studied, except one strain, were resistant to ampicillin. However, the strain that was susceptible to ampicillin had the same ß-lactamase gene as the ampicillinresistant strains. We compared ß-lactamase promoter region sequences among five strains, including both ampicillinresistant and -susceptible strains. In the susceptible strain, a nucleotide at position -19 in the methionine initiation codon for ß-lactamase was not present in the ampicillin-resistant strains. The genes in the region containing the gene VPA0477 were present in all of the tested strains, and LA-PCR analysis showed that the distance between VPA0474and VPA0479 in all of the V. parahaemolyticus samples was precisely 5.7 kb. In V. parahaemolyticus ß-lactamase,four important structural features that are conserved in Class A ß-lactamases were present in the deduced amino acid sequences. Taken together, our study demonstrates that V. parahaemolyticus ß-lactamase is included in the Class A ßlactamase group, and some nucleotides within the promoter region are of particular importance for ß-lactamase activity.
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