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금강 하구 해역의 해수에서 분리한 장염비브리오(Vibrio parahaemolyticus) 균의 특성 및 항균제 내성

Characterization and Antimicrobial Resistance of Vibrio parahaemolyticus Strains Isolated from Seawater of Geum River Estuary Area, West Coast of Korea

김희대|박권삼|이신혜
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)|2022.12|한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)|제55권 제6호|pp. 850-857

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참고문헌

키워드

Minimum inhibitory concentrationAntimicrobial resistanceVirulence genesVibrio parahaemolyticus
주제분류수산학

초록

Seventy-five Vibrio parahaemolyticus isolates from the surface seawater of the Geum River Estuary area, on the west coast of Korea, were analyzed for the presence of virulence genes and susceptibility to 17 different antimicrobials. All 75 isolates were examined for the presence of two virulence genes (tdh or trh) using polymerase chain reaction; Only one of the isolates possessed the tdh or trh gene. According to the results of disk diffusion susceptibility tests, all of the strains were resistant to penicillin G, 92.0% were resistant to ampicillin, 82.7% were resistant to amoxicillin, 2.7% were resistant to ciprofloxacin, 2.7% were resistant to trimethoprim, 1.3% were resistant to cephalothin, and 1.3% were resistant to erythromycin. However, all of the strains were susceptible to amikacin, cefoxitin, chloramphenicol, gentamycin, kanamycin, nalidixic acid, nitrofurantoin, rifampin, streptomycin, and tetracycline. The average minimum inhibitory concentrations for ampicillin for V. parahaemolyticus was 557.6 μg/mL. These results not only provide novel insight into the necessity for seawater sanitation in Geum river estuary area, but they help reduce the risk of contamination of antimicrobial-resistant bacteria.

Abstract

Seventy-five Vibrio parahaemolyticus isolates from the surface seawater of the Geum River Estuary area, on the west coast of Korea, were analyzed for the presence of virulence genes and susceptibility to 17 different antimicrobials. All 75 isolates were examined for the presence of two virulence genes (tdh or trh) using polymerase chain reaction; Only one of the isolates possessed the tdh or trh gene. According to the results of disk diffusion susceptibility tests, all of the strains were resistant to penicillin G, 92.0% were resistant to ampicillin, 82.7% were resistant to amoxicillin, 2.7% were resistant to ciprofloxacin, 2.7% were resistant to trimethoprim, 1.3% were resistant to cephalothin, and 1.3% were resistant to erythromycin. However, all of the strains were susceptible to amikacin, cefoxitin, chloramphenicol, gentamycin, kanamycin, nalidixic acid, nitrofurantoin, rifampin, streptomycin, and tetracycline. The average minimum inhibitory concentrations for ampicillin for V. parahaemolyticus was 557.6 μg/mL. These results not only provide novel insight into the necessity for seawater sanitation in Geum river estuary area, but they help reduce the risk of contamination of antimicrobial-resistant bacteria.

참고문헌 (44)

  1. Acar JF, "Antibiotics in Laboratory Medicine", pp.17~52 (1991), Williams and Wilkins
  2. 조의동, "해수 및 시판 수산물에서 분리한 장염비브리오균(Vibrio parahaemolyticus) 의 항균제 내성 및 최소발육억제농도의 규명", 한국수산과학회지, 52(6), pp.587~595 (2019), 한국수산과학회[DOI]
  3. Performance Standards for Antimicrobial Susceptibility Testing: 20th Informational Supplement, "CLSI", CLSI, ~, pp.M100-S20-U (2010)
  4. Deepanjali A, "Seasonal variation in abundance of total and pathogenic Vibrio parahaemolyticus bacteria in oysters along the southwest coast of India", Appl Environ Microbiol, 71, pp.3575~3580 (2005)
  5. Ellingsen AB, "Genetic characterization of trh positive Vibrio spp. isolated from Norway", Front Cell Infect Microbial, 3, pp.1~10 (2013)[DOI]
  6. Gutierrez West CK, "High frequency of virulence factor genes tdh, trh, and tlh in Vibrio parahaemolyticus strains isolated from a pristine estuary", Appl Environ Microbiol, 79, pp.2247~2252 (2013)[DOI]
  7. 한아름, "경기인천 연안에서 분리된 장염비브리오균의 항생제 내성 및 플라스미드 보유 현황", 미생물학회지, 48(1), pp.22~28 (2012), 한국미생물학회
  8. Honda T, "The pathogenicity of Vibrio parahaemolyticus and the role of the thermostable direct haemolysin and related haemolysins", Rev Med Microbiol, 4, pp.106~113 (1993)
  9. Jones JL, "Biochemical, serological, and virulence characterization of clinical and oyster Vibrio parahaemolyticus isolates", J Clin Microbiol, 50, pp.2343~2352 (2012)[DOI]
  10. Jo S, "Heavy metal and antibiotic co-resistance in Vibrio parahaemolyticus isolated from shellfish", Mar Pollut Bull, 156, pp.111246~ (2020)[DOI]
  11. Kang CH, "Characterization of Vibrio parahaemolyticus isolated from oysters in Korea: Resistance to various antibiotics and prevalence of virulence genes", Mar Pollut Bull, 118, pp.261~266 (2017)[DOI]
  12. Kang CH, "Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from oysters in Korea", Environ Sci Pollut Res Int, 23, pp.918~926 (2016)[DOI]
  13. Kang CH, "Antibiotic and heavy-metal resistance of Vibrio parahaemolyticus isolated from oysters in Korea", Mar Pollut Bull, 135, pp.69~74 (2018)[DOI]
  14. Kesici U, "Antimicrobial effects of local anaesthetics", Int Wound J, 16, pp.1029~1033 (2019)[DOI]
  15. 김수경, "양식 굴(Crassostrea gigas)에서 분리된 장염비브리오균의독소 유전자 보유 및 항균제 감수성", 한국수산과학회지, 49(2), pp.116~123 (2016), 한국수산과학회
  16. 김태옥, "완도해역 해수에서 분리한 장염비브리오(Vibrio parahaemolyticus)의 항균제 내성 및 병원성 유전자의 특징", 한국수산과학회지, 47(3), pp.220~226 (2014), 한국수산과학회
  17. 김태옥, "곰소만 해역 해수에서 분리한 장염비브리오(Vibrio parahaemolyticus)의 항균제 내성 및 최소발육억제농도의 규명", 한국수산과학회지, 49(5), pp.582~588 (2016), 한국수산과학회
  18. Kim YB, "Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene", J Clin Microbiol, 37, pp.1173~1177 (1999)[DOI]
  19. Kuhl SA, "Chromosomal map location of the methicillin resistance determinant in Staphylococcus aureus", J Bacteriol, 135, pp.460~465 (1978)[DOI]
  20. 이근우, "해수에서 분리한 장염비브리오의 항생제 내성 및 암피실린 내성 유전자의 동정", 한국수산과학회지, 43(6), pp.637~641 (2010), 한국수산과학회
  21. 이남형, "장염비브리오가 보유하는 ß-lactamase (VPA0477)의 유전학적 특성", 한국수산과학회지, 44(6), pp.597~604 (2011), 한국수산과학회
  22. Letchumanan V, "Vibrio parahaemolyticus: a review on the pathogenesis, prevalence, and advance molecular identification techniques", Front Microbiol, 5, pp.705~ (2014)[DOI]
  23. Li L, "Molecular mechanisms of Vibrio parahaemolyticus pathogenesis", Microbiol Res, 222, pp.43~51 (2019)[DOI]
  24. Lopatek M, "Prevalence and antimicrobial resistance of Vibrio parahaemolyticus isolated from raw hellfish in Poland", J Food Prot, 78, pp.1029~1033 (2015)[DOI]
  25. Makino K, "Genome sequence of Vibrio parahaemolyticus: A pathogenic mechanism distinct from that of V. cholerae", Lancet, 361, pp.743~749 (2003)[DOI]
  26. Martinez JL, "A global view of antibiotic resistance", FEMS Microbiol Rev, 33, pp.44~65 (2009)[DOI]
  27. "Food Poisoning Statistics" (2022)[URL]
  28. Mok JS, "Distribution and antimicrobial resistance of Vibrio parahaemolyticus isolated from fish and shrimp aquaculture farms along the Korean coast", Mar Pollut Bull, 171, pp.112785~ (2021)[DOI]
  29. Munita JM, "Mechanisms of antibiotic resistance", Microbiol Spectr, 4, pp.1~37 (2016)[DOI]
  30. No AR, "Rapid detection of Vibrio parahaemolyticus by PCR targeted to the histone-like nucleoid structure (H-NS) gene and its genetic characterization", Lett Appl Microbiol, 53, pp.127~133 (2011)[DOI]
  31. Ottaviani D, "Extensive investigation of antimicrobial resistance in Vibrio parahaemolyticus from shellfish and clinical sources, Italy", Int J Antimicrob Agents, 42, pp.191~193 (2013)[DOI]
  32. 박권삼, "β-Lactamase (VPA0477) 유전자를 표적으로 Polymerase chain reaction에 의한 장염비브리오(Vibrio parahaemolyticus)의 검출", 한국수산과학회지, 47(6), pp.740~744 (2014), 한국수산과학회[DOI]
  33. Park K, "Occurrence and virulence of Vibrio parahaemolyticus isolated from seawater and bivalve shellfish of the Gyeongnam coast, Korea, in 2004-2016", Mar Pollut Bull, 137, pp.382~387 (2018)[DOI]
  34. Park KS, "Genetic characterization of DNA region containing the trh and ure genes of Vibrio parahaemolyticus", Infect Immun, 68, pp.5742~5748 (2000)[DOI]
  35. Park KS, "Functional characterization of two type III secretion systems of Vibrio parahaemolyticus", Infect Immun, 72, pp.6659~6665 (2004)[DOI]
  36. Pazhani GP, "Trends in the epidemiology of pandemic and non-pandemic strains of Vibrio parahaemolyticus isolated from diarrheal patients in Kolkata, India", PLoS Negl Trop Dis, 8, pp.e2815~ (2014)[DOI]
  37. Raghunath P, "Roles of thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) in Vibrio parahaemolyticus", Front Microbiol, 5, pp.805~ (2015)[DOI]
  38. Rowe-Magnus DA, "The role of integrons in antibiotic resistance gene capture", Int J Med Microbiol, 292, pp.115~125 (2002)[DOI]
  39. 류아라, "서해안 패류에서 분리한 대장균 및 장염비브리오균의 항균제 내성 패턴", 한국수산과학회지, 50(6), pp.662~668 (2017), 한국수산과학회[DOI]
  40. Silva IP, "Antimicrobial resistance and potential virulence of Vibrio parahaemolyticus isolated from water and bivalve mollusks from Bahia, Brazil", Mar Pollut Bull, 131, pp.757~762 (2018)[DOI]
  41. Suthienkul O, "Urease production correlates with possession of the trh gene in Vibrio parahaemolyticus strains isolated in Thailand", J Infect Dis, 172, pp.1405~1408 (1995)[DOI]
  42. Wang R, "The pathogenesis, detection, and prevention of Vibrio parahaemolyticus", Front Microbiol, 6, pp.144~ (2015)[DOI]
  43. Xie T, "Prevalence and population analysis of Vibrio parahaemolyticus in aquatic products from South China markets", FEMS Microbiol Lett, 362, pp.1~7 (2015)[DOI]
  44. Zhang L, "Virulence determinants for Vibrio parahaemolyticus infection", Curr Opin Microbiol, 16, pp.70~77 (2013)[DOI]

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