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완도해역 해수에서 분리한 장염비브리오(Vibrio parahaemolyticus)의 항균제 내성 및 병원성 유전자의 특징

Antimicrobial-resistance Profiles and Virulence Genes of Vibrio para - haemolyticus Isolated from Seawater in the Wando Area

박권삼|조상만|엄인선|김태옥|김회대
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)|2014.06|한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)|제47권 제3호|pp. 220-226

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참고문헌

키워드

Minimum inhibitory concentrationAntimicrobial resistanceVirulence genesVibrio parahaemolyticus
주제분류수산학

초록

Sixty-sevenVibrio parahaemolyticus isolates from surface seawater from the Wando area, on the southern coast of Korea, were analyzed for their susceptibility to 15 different antimicrobials and the presence of virulence genes. According to the disk diffusion susceptibility test, all of the strains studied were resistant to ampicillin and oxacil- lin, while decreasing percentages were resistant to vancomycin (64.2%), streptomycin (56.7%), amikacin (31.3%), kanamycin (22.3%), cephalothin (20.9%), erythromycin (10.4%), ciprofloxacin (4.5%), and tetracycline (3.0%). All of the strains were susceptible to five antimicrobials: chloramphenicol, gentamycin, nalidixic acid, sulfamethoxazole/ trimethoprim, and trimethoprim. Fifty-nine isolates (88.1%) were resistant to three or more classes of antimicrobial and defined as multidrug resistant, and two strains were resistant to seven antimicrobial agents. The minimum inhibi- tory concentration (MIC) of the 67 V . parahaemolyticus isolates to ampicillin and oxacillin ranged from 512−2,048 and 64−512 µg/mL, respectively. All 67 isolates were also examined for the presence of the tdh and trh virulence genes using the polymerase chain reaction (PCR). However, no isolates possessed either tdh or trh . The VPA0477 (β-lactamase) gene, present in all of the tested strains, was validated as a new specific marker gene in PCR assays for the accurate detection and identification of V parahaemolyticus

Abstract

Sixty-sevenVibrio parahaemolyticus isolates from surface seawater from the Wando area, on the southern coast of Korea, were analyzed for their susceptibility to 15 different antimicrobials and the presence of virulence genes. According to the disk diffusion susceptibility test, all of the strains studied were resistant to ampicillin and oxacil- lin, while decreasing percentages were resistant to vancomycin (64.2%), streptomycin (56.7%), amikacin (31.3%), kanamycin (22.3%), cephalothin (20.9%), erythromycin (10.4%), ciprofloxacin (4.5%), and tetracycline (3.0%). All of the strains were susceptible to five antimicrobials: chloramphenicol, gentamycin, nalidixic acid, sulfamethoxazole/ trimethoprim, and trimethoprim. Fifty-nine isolates (88.1%) were resistant to three or more classes of antimicrobial and defined as multidrug resistant, and two strains were resistant to seven antimicrobial agents. The minimum inhibi- tory concentration (MIC) of the 67 V . parahaemolyticus isolates to ampicillin and oxacillin ranged from 512−2,048 and 64−512 µg/mL, respectively. All 67 isolates were also examined for the presence of the tdh and trh virulence genes using the polymerase chain reaction (PCR). However, no isolates possessed either tdh or trh . The VPA0477 (β-lactamase) gene, present in all of the tested strains, was validated as a new specific marker gene in PCR assays for the accurate detection and identification of V parahaemolyticus.

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