해수에서 분리한 장염비브리오의 항생제 내성 및 암피실린 내성 유전자의 동정
Antibiotic-Resistance Profiles and the Identification of the Ampicillin-Resistance Gene of Vibrio parahaemolyticus Isolated from Seawater
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The antibiotics-resistance profiles of 28 strains of Vibrio parahaemolyticus isolated from seawater were investigated. All of the strains studied were resistant to ampicillin (100%), but susceptible to 12 other antibiotics. The minimum inhibitory concentration (MIC) of V. parahaemolyticus to ampicillin was as high as 1,024-2,048 μg·mL-1. The phenotype of strain 8 changed from ampicillin-resistant to susceptible with an in-frame deletion mutant of VPA0477, a putative β-lactamase gene, and the MIC for ampicillin of the mutant strain was 1 μg·mL-1. In conclusion, our findings suggest that the VPA0477 gene acts as a β-lactamase in ampicillin-resistant V. parahaemolyticus strains.
Abstract
The antibiotics-resistance profiles of 28 strains of Vibrio parahaemolyticus isolated from seawater were investigated. All of the strains studied were resistant to ampicillin (100%), but susceptible to 12 other antibiotics. The minimum inhibitory concentration (MIC) of V. parahaemolyticus to ampicillin was as high as 1,024-2,048 μg·mL-1. The phenotype of strain 8 changed from ampicillin-resistant to susceptible with an in-frame deletion mutant of VPA0477, a putative β-lactamase gene, and the MIC for ampicillin of the mutant strain was 1 μg·mL-1. In conclusion, our findings suggest that the VPA0477 gene acts as a β-lactamase in ampicillin-resistant V. parahaemolyticus strains.
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