시판수산물에서 분리한 Vibrio parahaemolyticus의 병원성 인자와 항균제 내성 현황
Virulence Factors and Antimicrobial Resistance of Vibrio parahaemolyticus Isolated from Commercial Fisheries Products
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Vibrio parahaemolyticus causes food poisoning, mainly via marine fisheries products. We investigated the virulence factors and drug resistance of V. parahaemolyticus isolated from fisheries products purchased from the Yeosu Fisheries Market. The isolates were identified using a variety of biochemical tests and the detection of toxR and hns gene. The presence of the virulence factor-encoding genes tdh and trh in the isolates was also investigated by PCR. The resistance of the isolates to 13 antibacterial agents was tested using the disc-diffusion method and carriage of β-lactamase genes and class 1 integrons by ampicillin-resistant isolates was investigated by PCR. Four of seventeen isolates identified as V. parahaemolyticus by biochemical tests produced a species-specific PCR band. Those isolates showed >98% 16S rRNA gene sequence homology with V. parahaemolyticus and only one isolate harbored the tdh gene. All of the V. parahaemolyticus isolates were resistant to ampicillin and amoxicillin; moreover, VPA0477, a class A β-lactamase gene, and class 1 integrons were detected. Therefore, V. parahaemolyticus from fisheries products represents a low risk to human health. Also, V. parahaemolyticus is likely to develop multidrug resistance because it has class 1 integrons.
Abstract
Vibrio parahaemolyticus causes food poisoning, mainly via marine fisheries products. We investigated the virulence factors and drug resistance of V. parahaemolyticus isolated from fisheries products purchased from the Yeosu Fisheries Market. The isolates were identified using a variety of biochemical tests and the detection of toxR and hns gene. The presence of the virulence factor-encoding genes tdh and trh in the isolates was also investigated by PCR. The resistance of the isolates to 13 antibacterial agents was tested using the disc-diffusion method and carriage of β-lactamase genes and class 1 integrons by ampicillin-resistant isolates was investigated by PCR. Four of seventeen isolates identified as V. parahaemolyticus by biochemical tests produced a species-specific PCR band. Those isolates showed >98% 16S rRNA gene sequence homology with V. parahaemolyticus and only one isolate harbored the tdh gene. All of the V. parahaemolyticus isolates were resistant to ampicillin and amoxicillin; moreover, VPA0477, a class A β-lactamase gene, and class 1 integrons were detected. Therefore, V. parahaemolyticus from fisheries products represents a low risk to human health. Also, V. parahaemolyticus is likely to develop multidrug resistance because it has class 1 integrons.
참고문헌 (35)
- Ambler RP, "The structure of β-lactamases", Phil Trans R Soc Lond, B289, pp.321~331 (1980)
- G. Arlet, "Construction by polymerase chain reaction and intragenic DNA probes for three main types of transferable β-lactamases (TEM, SHV, CARB)", FEMS Microbiology Letters, 82(1), pp.19~25 (1991), Oxford University Press (OUP)[DOI]
- Barcelos DHF, "Emergence and dispersion of resistance genes by the aquatic environment : a review", Pollution, 4, pp.305~315 (2018)[DOI]
- Bush K, "Updated functional classification of β-lactamases", Antimicrob Agents Chemother, 54, pp.969~976 (2010)[DOI]
- Ceccarelli D, "New cluster pf plasmid-located class I integrons in Vibrio cholera O1 and a dfrA15 cassette containing integron in V. parahaemolyticus isolated in Angola", Antimicrob Agents Chemother, 50, pp.2493~2499 (2006)
- Performance standards for antimicrobial susceptibility testing, "Clinical and Laboratory Standards Institute", CLSI, ~, pp.CLSI document M100-S23 (M02-A11) (2013)
- Dallenne C, "Development of a set of multiplex PCR assays for the de-tection of genes encoding important β-lactamases in Enterobacteriaceae", J Antimicrob Chemother, 65, pp.490~495 (2010)[DOI]
- Ghuysen JM, "Molecular structures of penicillin-binding proteins and β-lactamases", Trends Microbiol, 2, pp.372~380 (1994)
- Kang CH, "Characterization of Vibrio parahaemolyticus isolated from oysters in Korea : Resistance to various antibiotics and prevalence of virulence genes", Mar Pollut Bull, 118, pp.261~266 (2017)[DOI]
- Kim YB, "Identification of Vibrio parahaemolyticus strains at the speices level by PCR targeted to the toxR gene", J Clin Microbiol, 37, pp.1173~1177 (1999)
- 김태옥, "곰소만 해역 해수에서 분리한 장염비브리오(Vibrio parahaemolyticus)의 항균제 내성 및 최소발육억제농도의 규명", 한국수산과학회지, 49(5), pp.582~588 (2016), 한국수산과학회
- "One health collaboration project proposal" (2016)[URL]
- "Status of food poisoning and measures. Food industry 72-84" (1999)[URL]
- Labia R, "Properties of three carbenicillin-hydrolysing β-lactamases (CARB) from Pseudomonas aeruginosa: identification of a new enzyme", J Antimicrob Chemother, 7, pp.49~56 (1981)
- 이향, "어패류에서 분리한 장염비브리오균의 ？항균제 감수성 및 분포", 한국환경보건학회지, 33(1), pp.16~20 (2007), 한국환경보건학회
- 이근우, "해수에서 분리한 장염비브리오의 항생제 내성 및 암피실린 내성 유전자의 동정", 한국수산과학회지, 43(6), pp.637~641 (2010), 한국수산과학회
- Levesque C, "PCR mapping of integrons reveals several novel combinations of resistance genes", Antimicrob Agents Chemother, 39, pp.185~191 (1995)[DOI]
- Ruichao Li, "First detection of AmpC β-lactamase blaCMY-2 on a conjugative IncA", C plasmid in a Vibrio parahaemolyticus isolates of food origin, 2015, pp.59(7), 4106~4111[DOI]
- Liu M, "Molecular characterisation of a multidrug resistance conjugative plasmid from Vibrio parahaemolyticus", Int J Microbiol Agents, 42, pp.575~579 (2013)[DOI]
- Makino K, "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae", Lancet, 361, pp.743~749 (2003)
- "Notice on full revision of food standards and regulations, Chapter 7, General test methods (No. 2016-154)" (2016)[URL]
- "Occurrence of food poisoning by causes and patients - year and city: 2008-2017" (2018)[URL]
- Miyamoto Y, "In vitro hemolytic characteristic of Vibrio parahaemolyticus : its close correlation with human pathogenicity", J Bacterial, 100, pp.1147~1149 (1969)
- NIFS(National Institute of Fisheries Science), "Antibiotic resistant bacteria inspection manual (11-1192266-000195-01)", GMK communication, 34~38 (2017)
- Nishibuchi M, "Thermostable direct hemolysin gene of Vibrio parahaemolyticus : a virulence gene acquired by a marine bacterium", Infect Immun, 63, pp.2093~2099 (1995)
- No AR, "Rapid detection of Vibrio parahaemolyticus by PCR targeted to the histone-like nucleoid structure(H-NS)gene and its genetic characterization", Lett Appl Microbiol, 53, pp.127~133 (2011)[DOI]
- Oh EG, "Antimicrobial resistance of Vibrio parahaemolyticus and Vibrio alginolyticus strains isolated from farmed fish in Korea from 2005 through 2007", J Food Prot, 74, pp.380~386 (2011)[DOI]
- 박권삼, "β-Lactamase (VPA0477) 유전자를 표적으로 Polymerase chain reaction에 의한 장염비브리오(Vibrio parahaemolyticus)의 검출", 한국수산과학회지, 47(6), pp.740~744 (2014), 한국수산과학회[DOI]
- 박용수, "남해안 패류양식장에서 분리한 Vibrio parahaemolyticus의 병원인자분포 및 항균제내성", 한국수산과학회지, 49(4), pp.460~466 (2016), 한국수산과학회
- Pazhani GP, "Trend in the epidemiology of pandemic and nonpandemic strain of Vibrio parahaemolyticus isolated from diarrheal patients in Kolkata, India", PLoS Negl Trop Dis, 8, pp.e2815~ (2014)[DOI]
- 류아라, "서해안 패류에서 분리한 대장균 및 장염비브리오균의 항균제 내성 패턴", 한국수산과학회지, 50(6), pp.662~668 (2017), 한국수산과학회[DOI]
- 류승희, "시판 어패류에서 분리한 장염 비브리오균의 항생제 감수성 특성", 한국식품과학회지, 42(4), pp.508~513 (2010), 한국식품과학회
- Sakazaki R, "Studies of the enteropathogenic, facultatively halophilic bacteria, Vibrio parahaemolyticus. III. Enteropathogenicity", Jpn J Med Sci Biol, 21, pp.325~331 (1968)
- Weisburg WG, "16S ribosomal DNA amplification for phylogenetic study", J Bacteriol, 173, pp.679~703 (1991)[DOI]
- Yeung PSM, "Epidemiology, pathogenesis, and prevention of foodborne Vibrio parahaemolyticus", Foodborne Pathog Dis, 1, pp.74~88 (2004)