Antioxidant and skin health-enhancing activities of wild indigo (Baptisia tinctoria) root extracts
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This study aimed to investigate the effects of wild indigo (Baptisia tinctoria) root extract as a functional food material, in terms of antioxidant and skin health-related activities. Both water and ethanol (50/50, v/v) extracts showed high phenol content. They were evaluated for antioxidant activity, such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), antioxidant protection factor (PF), and thiobarbituric acid reactive substances (TBARS). While the DPPH radical scavenging activities of water and ethanol extracts were 84.23% and 89.30%, respectively, at 200 μg/mL total phenol content (TPC), the ABTS radical cation scavenging activities were 51.39% and 48.10%, respectively. The water and ethanol extracts also showed desirable antioxidant PF levels of 1.18 and 1.16, respectively. Furthermore, the antioxidant effects on TBARS significantly increased by 37.31% and 51.43%, respectively, in 200 μg/mL of water and ethanol extracts. Both the extracts showed high dose-dependent collagenase inhibitory activities, up to 27.60% and 77.39%, respectively. The ethanol extract showed an elastase inhibitory activity of 37.21%; the tyrosinase inhibitory effect (which facilitates natural skin-whitening) was 59.26% at 200 μg/mL TPC. The hyaluronidase activity inhibition, related to anti-inflammatory activity, was 20.02% for the water extract and 14.30% for the ethanol extract, at 200 μg/mL TPC. Therefore, B. tinctoria extract has potential applications in cosmetics and food supplements due to its antioxidant and skin health promoting activities.
Abstract
This study aimed to investigate the effects of wild indigo (Baptisia tinctoria) root extract as a functional food material, in terms of antioxidant and skin health-related activities. Both water and ethanol (50/50, v/v) extracts showed high phenol content. They were evaluated for antioxidant activity, such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), antioxidant protection factor (PF), and thiobarbituric acid reactive substances (TBARS). While the DPPH radical scavenging activities of water and ethanol extracts were 84.23% and 89.30%, respectively, at 200 μg/mL total phenol content (TPC), the ABTS radical cation scavenging activities were 51.39% and 48.10%, respectively. The water and ethanol extracts also showed desirable antioxidant PF levels of 1.18 and 1.16, respectively. Furthermore, the antioxidant effects on TBARS significantly increased by 37.31% and 51.43%, respectively, in 200 μg/mL of water and ethanol extracts. Both the extracts showed high dose-dependent collagenase inhibitory activities, up to 27.60% and 77.39%, respectively. The ethanol extract showed an elastase inhibitory activity of 37.21%; the tyrosinase inhibitory effect (which facilitates natural skin-whitening) was 59.26% at 200 μg/mL TPC. The hyaluronidase activity inhibition, related to anti-inflammatory activity, was 20.02% for the water extract and 14.30% for the ethanol extract, at 200 μg/mL TPC. Therefore, B. tinctoria extract has potential applications in cosmetics and food supplements due to its antioxidant and skin health promoting activities.
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