Antioxidant Capacity and Anti-Wrinkle Efficacy of Coreopsis lanceolate L. Methanol Extract in a Cell-free System
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The aims of the current study were to investigate the antioxidant capacity and anti-wrinkle efficacy of Coreopsis lanceolate L. methanol extract (CLME) in a cell-free system. Total polyphenolics and flavonoids in the CLME were 351.6 TAE mg/g and 382.5 RE mg/g, respectively. At the concentration of 1,000 μg/mL, the electron-donating ability of CLME was 94.8% and SC50 was calculated to be 83.63 μg/mL. Collagenase and elastase activity inhibition of CLME at 1,000 μg/mL was 73.2% and 79.4%, respectively. The IC50 of collagenase and elastase activity was calculated to be 460.5 μg/mL and 529.2 μg/mL, respectively, which was similar to or even superior to the ascorbic acid (AA) positive control (448.3 μg/mL and over 1,000 μg/mL, respectively). As determined with an MTT assay, the maximum permissible level for treating CLME to HaCaT keratinocytes was shown to be 100 μg/mL.
Abstract
The aims of the current study were to investigate the antioxidant capacity and anti-wrinkle efficacy of Coreopsis lanceolate L. methanol extract (CLME) in a cell-free system. Total polyphenolics and flavonoids in the CLME were 351.6 TAE mg/g and 382.5 RE mg/g, respectively. At the concentration of 1,000 μg/mL, the electron-donating ability of CLME was 94.8% and SC50 was calculated to be 83.63 μg/mL. Collagenase and elastase activity inhibition of CLME at 1,000 μg/mL was 73.2% and 79.4%, respectively. The IC50 of collagenase and elastase activity was calculated to be 460.5 μg/mL and 529.2 μg/mL, respectively, which was similar to or even superior to the ascorbic acid (AA) positive control (448.3 μg/mL and over 1,000 μg/mL, respectively). As determined with an MTT assay, the maximum permissible level for treating CLME to HaCaT keratinocytes was shown to be 100 μg/mL.
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