자동 요침사분석기 iQ200의 평가
Evaluation of iQ200 Automated Urine Microscopy Analyzer
1
조회
0
다운로드
6
인용
0
참고문헌
초록
Abstract
Background : Microscopic examination of urine sediment is one of the most commonly performed tests in the clinical laboratory. However, manual microscopic sediment examination is labor-intensive, time-consuming and imprecise. In this study, we evaluated the analytical performance and clinical usefulness of a recently introduced image-based automated urinalysis system, Iris iQ200(Iris Diagnostics, USA). Methods : We assessed the iQ200 for linearity, precision and carryover rate using patient’s samples and quality control materials. On 337 urine samples, urine sediment analyses performed by the iQ200 were compared with manual microscopy results. Results : The iQ200 showed a good linearity (r2>0.99) for all cellular components analyzed. Within-run and total CVs on urine specimens and quality control samples were less than 10% except for within-run CV for the samples with low concentration of the squamous epithelial cells. The carryover rates were 0.21% for RBCs and 1.92% for WBCs. The agreement rates within one grade between the iQ200 and manual microscopy for RBCs, WBCs, and squamous epithelial cells were 93.8%, 94.2% and 96.9%, respectively. Conclusions : Since the iQ200 showed a reliable analytical performance and good concordance with manual microscopy, it could be useful in the clinical practice as a screening procedure. (Korean J Lab Med 2008;28:267-73)
참고문헌 (18)
- Fuller CE, "Basic examination of urine in: Clinical diagnosis and management by laboratory methods", pp.367~402 (2001), WB Saunders
- Ferris JA, "Comparison and standardization of the urine microscopic examination", Lab Med, 14, pp.659~662 (1983)
- Huussen J, "The (fixed) urinary sediment, a simple and useful diagnostic tool in patients with haematuria", Neth J Med, 62, pp.4~9 (2004)
- Elin RJ, "Comparison of automated and manual methods for urinalysis", Am J Clin Pathol, 86, pp.731~737 (1986)
- Carlson DA, "Automated urinalysis", Clin Lab Med, 8, pp.449~461 (1988)
- Wah DT, "Analytic performance of the iQ200 automated urine microscopy analyzer and comparison with manual counts using Fuchs-Rosenthal cell chambers", Am J Clin Pathol, 123, pp.290~296 (2005)
- Lamchiagdhase P, "Urine sediment examination: a comparison between the manual method and the iQ200 automated urine microscopy analyzer", Clin Chim Acta, 358, pp.167~174 (2005)
- Chien TI, "Urine sediment examination: a comparison of automated urinalysis systems and manual microscopy", Clin Chim Acta, 384, pp.28~34 (2007)
- Shayanfar N, "Automated urinalysis: first experiences and a comparison between the Iris iQ200 urine microscopy system, the Sysmex UF-100 flow cytometer and manual microscopic particle counting", Clin Chem Lab Med, 45, pp.1251~1256 (2007)
- Okada H, "Automated urinalysis. Evaluation of the Sysmex UF-50", Am J Clin Pathol, 115, pp.605~610 (2001)
- "National Committee for Clinical Laboratory Standards. Evaluation of the linearity of quantitative measurement procedures: a statistical approach; approved guideline. NCCLS document EP6-A. Wayne, PA: National Committee for Clinical Laboratory Standards, 2003", ~ (2003)
- National Committee for Clinical Laboratory Standards, "Evaluation of the precision performance of clinical chemistry devices; approved guideline" (1999), National Committee for Clinical Laboratory Standards
- Pesce MA, "Laboratory automation in: Clinical Chemistry", pp.294~ (2003), Mosby
- Ottiger C, "Quantitative urine particle analysis: integrative approach for the optimal combination of automation with UF-100 and microscopic review with KOVA cell chamber", Clin Chem, 49, pp.617~623 (2003)
- Roe CE, "Evaluation of the Yellow IRIS. An automated method for urinalysis", Am J Clin Pathol, 86, pp.661~665 (1986)
- Deindoerfer FH, "‘The Yellow IRIS’’ urinalysis workstation--the first commercial application of ‘‘automated intelligent microscopy’", Clin Chem, 31, pp.1491~1499 (1985)
- Wargotz ES, "Urine sediment analysis by the Yellow IRIS automated urinalysis workstation", Am J Clin Pathol, 88, pp.746~748 (1987)
- Kim DC, "Laboratory evaluation of fully automated urine cell analyzer Sysmex UF-100", J Clin Pathol & Quality Control, 23, pp.299~306 (2001)