Comparison of IRIS Iq200, UF-1000i, and Cobas u701 Module Automated Urine Sediment Analyzers
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Background: We sought to compare the performance of three commercially available automated urine sediment analyzers that represent the current urine sediment analysis technology. Methods: A total of 232 patient samples were analyzed using manual microscopy and three automated analyzers: IRIS Iq200 (Beckman Coulter, USA), UF-1000i (Sysmex, Japan), and Cobas u701 (Roche, Switzerland). We analyzed precision, linearity, carry-over, concordance rate, and agreement between the three analyzers and manual microscopy. Results: The repeatability and within-laboratory precision showed results similar to those of previous studies. All analyzers showed excellent linearity. The carry-over rates were within 1%. The correlation coefficient (r) between the three analyzers and manual microscopy was good. Regarding red blood cell (RBC), the UF-1000i showed a better concordance rate (90.52%) with manual microscopy than the other two analyzers and the agreement was substantial for UF-1000i (κ=0.63) and IRIS Iq200 (κ=0.61). Regarding white blood cell (WBC), Cobas u701 showed the best concordance rate (96.55%) and the agreement was moderate for IRIS Iq200 (κ=0.57) and Cobas u701 (κ=0.56), and fair for UF-1000i (κ=0.47). Regarding epithelial cell (EPI), IRIS Iq200 showed the highest concordance rate (99.2%) and the agreement was moderate for IRIS Iq200 (κ=0.59) and Cobas u701 (κ=0.54), and fair for UF-1000i (κ=0.40). Conclusions: IRIS Iq200 offered the best agreement with manual microscopy for WBC and EPI count, while UF-1000i showed a better agreement for RBC count. The agreement is insufficient for fully replacing the manual microscopy.
Abstract
Background: We sought to compare the performance of three commercially available automated urine sediment analyzers that represent the current urine sediment analysis technology. Methods: A total of 232 patient samples were analyzed using manual microscopy and three automated analyzers: IRIS Iq200 (Beckman Coulter, USA), UF-1000i (Sysmex, Japan), and Cobas u701 (Roche, Switzerland). We analyzed precision, linearity, carry-over, concordance rate, and agreement between the three analyzers and manual microscopy. Results: The repeatability and within-laboratory precision showed results similar to those of previous studies. All analyzers showed excellent linearity. The carry-over rates were within 1%. The correlation coefficient (r) between the three analyzers and manual microscopy was good. Regarding red blood cell (RBC), the UF-1000i showed a better concordance rate (90.52%) with manual microscopy than the other two analyzers and the agreement was substantial for UF-1000i (κ=0.63) and IRIS Iq200 (κ=0.61). Regarding white blood cell (WBC), Cobas u701 showed the best concordance rate (96.55%) and the agreement was moderate for IRIS Iq200 (κ=0.57) and Cobas u701 (κ=0.56), and fair for UF-1000i (κ=0.47). Regarding epithelial cell (EPI), IRIS Iq200 showed the highest concordance rate (99.2%) and the agreement was moderate for IRIS Iq200 (κ=0.59) and Cobas u701 (κ=0.54), and fair for UF-1000i (κ=0.40). Conclusions: IRIS Iq200 offered the best agreement with manual microscopy for WBC and EPI count, while UF-1000i showed a better agreement for RBC count. The agreement is insufficient for fully replacing the manual microscopy.
참고문헌 (39)
- Clinical and Laboratory Standards Institute, "Urinalysis; Approved Guideline" (2009), Clinical and Laboratory Standards Institute
- McPherson RA, "Henry’s clinical diagnosis and management by laboratory methods", pp.442~ (2017), Elsevier-Saunders
- Perazella MA, "The urine sediment as a biomarker of kidney disease", Am J Kidney Dis, 66, pp.748~755 (2015)
- Becker GJ, "Advances in urine microscopy", Am J Kidney Dis, 67, pp.954~964 (2016)
- Manoni F, "Pediatric reference values for urine particle quantification by using automated flow cytometer : results of a multicenter study of Italian urinalysis group", Clin Biochem, 46, pp.1820~1824 (2013)
- Kouri TT, "Towards European urinalysis guidelines. Introduction of a project under European Confederation of Laboratory Medicine", Clin Chim Acta, 297, pp.305~311 (2000)
- Du J, "Establishment and development of the personalized criteria for microscopic review following multiple automated routine urinalysis systems", Clin Chim Acta, 444, pp.221~228 (2015)
- Winkel P, "Urine microscopy, an Ⅲ-defined method, examined by a multifactorial technique", Clin Chem, 20, pp.436~439 (1974)
- Chase J, "Urine sediment examination : Potential impact of red and white blood cell counts using different sediment methods", Vet Clin Pathol, 47, pp.608~616 (2018)
- Wargotz ES, "Urine sediment analysis by the Yellow IRIS automated urinalysis workstation", Am J Clin Pathol, 88, pp.746~748 (1987)
- Deindoerfer FH, ""The Yellow IRIS"urinalysis workstation--the first commercial application of"automated intelligent microscopy"", Clin Chem, 31, pp.1491~1499 (1985)
- Roe CE, "Evaluation of the Yellow IRIS® : an automated method for urinalysis", Am J Clin Pathol, 86, pp.661~665 (1986)
- Mayo S, "Clinical laboratory automated urinalysis : comparison among automated microscopy, flow cytometry, two test strips analyzers, and manual microscopic examination of the urine sediments", J Clin Lab Anal, 22, pp.262~270 (2008)
- Chien TI, "Urine sediment examination : a comparison of automated urinalysis systems and manual microscopy", Clin Chim Acta, 384, pp.28~34 (2007)
- Lee W, "Comparison of the automated cobas u701 urine microscopy and UF-1000i flow cytometry systems and manual microscopy in the examination of urine sediments", J Clin Lab Anal, 30, pp.663~671 (2016)
- Cho J, "Comparison of five automated urine sediment analyzers with manual microscopy for accurate identification of urine sediment", Clin Chem Lab Med, 57, pp.1744~1753 (2019)
- Wang J, "Evaluation of the Sysmex UF-1000i for the diagnosis of urinary tract infection", Am J Clin Pathol, 133, pp.577~582 (2010)
- Manoni F, "Urine particle evaluation : a comparison between the UF-1000i and quantitative microscopy", Clin Chem Lab Med, 48, pp.1107~1111 (2010)
- Manoni F, "Cutoff values for bacteria and leukocytes for urine flow cytometer Sysmex UF-1000i in urinary tract infections", Diagn Microbiol Infect Dis, 65, pp.103~107 (2009)
- Bakan E, "Comparison of Cobas 6500 and Iris IQ200 fully-automated urine analyzers to manual urine microscopy", Biochem Med(Zagreb), 26, pp.365~375 (2016)
- Bakan E, "Evaluation of the analytical performances of Cobas 6500 and Sysmex UN series automated urinalysis systems with manual microscopic particle counting", Biochem Med(Zagreb), 28, pp.020712~ (2018)
- Wesarachkitti B, "Performance evaluation and comparison of the fully automated urinalysis analyzers UX-2000 and Cobas 6500", Lab Med, 47, pp.124~133 (2016)
- Linko S, "Analytical performance of the Iris iQ200 automated urine microscopy analyzer", Clin Chim Acta, 372, pp.54~64 (2006)
- Wah DT, "Analytic performance of the iQ200 automated urine microscopy analyzer and comparison with manual counts using Fuchs-Rosenthal cell chambers", Am J Clin Pathol, 123, pp.290~296 (2005)
- Clinical and Laboratory Standards Institute, "Evaluation of precision performance of quantitative measurement methods; Approved guideline" (2004), Clinical and Laboratory Standards Institute
- Clinical and Laboratory Standards Institute, "Evaluation of the linearity of quantitative measurement procedures: A statistical approach; Approved guideline" (2003), Clinical and Laboratory Standards Institute
- Kaplan LA, "Clinical chemistry: theory, analysis, correlation", pp.294~ (2003), Mosby
- Landis JR, "The measurement of observer agreement for categorical data", Biometrics, 33, pp.159~174 (1977)
- Budak YU, "Comparison of three automated systems for urine chemistry and sediment analysis in routine laboratory practice", Clin Lab, 57, pp.47~52 (2011)
- Cui M, "Performance verification of the Iris iQ200 Sprint automated urine microscopy analyzer in a hospital routine laboratory", Clin Lab, 63, pp.1607~1612 (2017)
- 박주원, "자동 요침사분석기 iQ200의 평가", Annals of Laboratory Medicine, 28(4), pp.267~273 (2008), 대한진단검사의학회
- Altekin E, "New generation IQ-200 automated urine microscopy analyzer compared with KOVA cell chamber", J Clin Lab Anal, 24, pp.67~71 (2010)
- Ottiger C, "Quantitative urine particle analysis : integrative approach for the optimal combination of automation with UF-100 and microscopic review with KOVA cell chamber", Clinl Chem, 49, pp.617~623 (2003)
- Langlois MR, "Automated flow cytometry compared with an automated dipstick reader for urinalysis", Clin Chem, 45, pp.118~122 (1999)
- Ben-Ezra J, "Evaluation of the Sysmex UF-100 automated urinalysis analyzer", Clin Chem, 44, pp.92~95 (1998)
- Zaman Z, "Urine sediment analysis : Analytical and diagnostic performance of sediMAX® — A new automated microscopy image-based urine sediment analyser", Clin Chima Acta, 411, pp.147~154 (2010)
- Enko D, "Comparison of the diagnostic performance of two automated urine sediment analyzers with manual phase-contrast microscopy", Clin Chem Lab Med, 58, pp.268~273 (2020)
- Bartosova K, "Analysis of four automated urinalysis systems compared to reference methods", Clin Lab, 62, pp.2115~2123 (2016)
- Cao Y, "UrineCART, a machine learning method for establishment of review rules based on UF-1000i flow cytometry and dipstick or reflectance photometer", Clin Chem Lab Med, 50, pp.2155~2161 (2012)