Necessity of Reticulocyte Calibration for More Accurate and Precise Results
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Dear Editor, Reticulocyte count and immature reticulocyte fraction (IRF) are known to be accurate indices of erythropoietic activity [1]. Apart from clinical utility, these parameters are also useful for detecting blood doping in the athletic field [2, 3]. Recently, the XN system (Sysmex, Kobe, Japan) was introduced to the market [4]. Although this new instrument uses the same analytical method for reticulocyte counting as the previous version of the XE system, the number of red blood cells (RBCs) that could be counted during reticulocyte counting was increased to improve the precision; in addition, the XN system introduced a new calibrator (XN CAL, Sysmex) to harmonize the reticulocyte count among different analyzers. Increase in the number of RBCs that can be counted and calibration of the reticulocyte count could contribute to the interchangeability of the results, not only among hospitals but also among anti-doping laboratories. The aim of this study was to demonstrate and quantify the improvement in the performance of reticulocyte parameters in the XN series with calibration.
Abstract
Dear Editor, Reticulocyte count and immature reticulocyte fraction (IRF) are known to be accurate indices of erythropoietic activity [1]. Apart from clinical utility, these parameters are also useful for detecting blood doping in the athletic field [2, 3]. Recently, the XN system (Sysmex, Kobe, Japan) was introduced to the market [4]. Although this new instrument uses the same analytical method for reticulocyte counting as the previous version of the XE system, the number of red blood cells (RBCs) that could be counted during reticulocyte counting was increased to improve the precision; in addition, the XN system introduced a new calibrator (XN CAL, Sysmex) to harmonize the reticulocyte count among different analyzers. Increase in the number of RBCs that can be counted and calibration of the reticulocyte count could contribute to the interchangeability of the results, not only among hospitals but also among anti-doping laboratories. The aim of this study was to demonstrate and quantify the improvement in the performance of reticulocyte parameters in the XN series with calibration.
참고문헌 (9)
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- CLSI, "Methods for Reticulocyte Counting (Automated Blood Cell Counters, Flow Cytometry, and Supravital Dyes). 2nd ed. CLSI supplement EP44-A2", Clinical and Laboratory Standards Institute, ~ (2004)
- LSI, "Evaluation of Precision of Quantitative Measurement Procedures. 3rd ed. CLSI supplement EP05-A3", Clinical and Laboratory Standards Institute, ~ (2014)
- Burtis CA, "Tietz textbook of clinical chemistry and molecular diagnostics. 5th ed." (2013), Saunders
- Briggs C, "Comparison of the automated reticulocyte counts and immature reticulocyte fraction measurements obtained with the ABX Pentra 120 Retic blood analyzer and the Sysmex XE-2100 automated hematology analyzer", Lab Hematol, 7, pp.75~80 (2001)