XE-2100을 이용한 망상적혈구수와 미성숙망상적혈구 분획의 측정

Abstract

We evaluated reticulocyte counting and measurement of inlmature reticulocyte fraction (IRF) with XE-2100 automated CBC analyzer (Sysmex Co. Japan). Reticulocyte counts and inlmature reticulocyte fraction were compared with those obtained by the R-3000 automated reticulocyte analyzer (Sysmex Co. Japan). To establish reticulocyte and immature reticulocyte fraction reference values, we measured reticulocyte count and immature reticulocyte fraction in healthy adult population. We performed with-in day precision studies on 3 patients showing low, medium, and high percentage of reticulocyte and between-day precision studies with Q.C material (Re-check Sysmex Co. Japan). The result were summarized as follows1. Comparison of XE-2100 and R-3000 : There was some variation of correlation between the level of reticulocyte counts such as r=0.75 in less than 0.5% group, r=0.75 in 0.51-1.80% group and r=0.78 in more than 1.81% group. 2. Reference value of reticulocyte count : Adult 0.6% - 2.0%,Reference value of inlmature reticulocyte fraction : Adult 1.1 % - 11.3 % 3. Precision : With-in day precision (n=9) : The C.V of the reticulocyte counts for low (mean0.80%), medium (mean2.15%), and high (mean 14.69%) reticulocyte count specimens was 7.27%, 4.08%, and 3.64%, respectively. Between day precision (n=23) : The C.V of the reticu10cyte and immatlure reticulocyte fraction were 2.84% and 12.6%, respectively.