The Sysmex XN-2000 Hematology Autoanalyzer Provides a Highly Accurate Platelet Count than the Former Sysmex XE-2100 System Based on Comparison with the CD41/CD61 Immunoplatelet Reference Method of Flow Cytometry

Abstract

Dear Editor The recently launched hematology autoanalyzer Sysmex XN- 2000 (Sysmex, Kobe, Japan) provides a new method (PLT-F method) for platelet counting based on a fluorescent RNA stain- ing dye named oxazine [1, 2]. The main advantage of the XN- 2000’s PLT-F method over the former instrument XE-2100’s op- tical method (PLT-O method) is the ability to identify apoptotic white cells and not count them as platelets [2]. The XN-2000’s PLT-F method was reported to estimate platelets in thrombocy- topenic samples more accurately than does the PLT-O method [1]. However, the performance of XN-2000’s PLT-F method still needs to be evaluated, including samples with broad range of platelet counts. We performed correlation and bias analyses comparing the platelet counts measured by the XN-2000 and XE-2100 autoanalyzers with those calculated by the immuno- platelet reference method (IRM) of flow cytometry.