Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images
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The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.
Abstract
The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.
참고문헌 (4)
- M. M. Stevens, "Exploring and engineering the cell surface interface", Science, 310, pp.1135~ (2005)
- J. L. Tan, "Cells lying on a bed of microneedles : an approach to isolate mechanical force", Proc. Natl. Acad. Sci, 100, pp.1484~ (2003)
- L. Trichet, "Evidence of a large-scale mechanosensing mechanism for cellular adaptation to substrate stiffness", Proc. Natl. Acad. Sci, 109, pp.6933~ (2012)
- Y. S. Yun, "Quantitative correlation of nanotopography with cell spreading via focal adhesions using adipose-derived stem cells", Adv. Biosyst, 4, pp.2000092~ (2020)